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PMID:10036776

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Citation

Gupta, R, Huang, Y, Kieber, J and Luan, S (1998) Identification of a dual-specificity protein phosphatase that inactivates a MAP kinase from Arabidopsis. Plant J. 16:581-9

Abstract

Mitogen-activated protein kinases (MAPKs) play a key role in plant responses to stress and pathogens. Activation and inactivation of MAPKs involve phosphorylation and dephosphorylation on both threonine and tyrosine residues in the kinase domain. Here we report the identification of an Arabidopsis gene encoding a dual-specificity protein phosphatase capable of hydrolysing both phosphoserine/threonine and phosphotyrosine in protein substrates. This enzyme, designated AtDsPTP1 (Arabidopsis thaliana dual-specificity protein tyrosine phosphatase), dephosphorylated and inactivated AtMPK4, a MAPK member from the same plant. Replacement of a highly conserved cysteine by serine abolished phosphatase activity of AtDsPTP1, indicating a conserved catalytic mechanism of dual-specificity protein phosphatases from all eukaryotes.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Arabidopsis/enzymology; Arabidopsis/genetics; Base Sequence; Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors; Catalytic Domain/genetics; DNA Primers/genetics; DNA, Complementary/genetics; DNA, Plant/genetics; Enzyme Inhibitors/metabolism; Genes, Plant; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Plant/genetics; RNA, Plant/metabolism; Sequence Homology, Amino Acid; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DUS1

enables

GO:0008138: protein tyrosine/serine/threonine phosphatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:DUS1

NOT|involved_in

GO:0034605: cellular response to heat

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

ARATH:DUS1

NOT|involved_in

GO:0009611: response to wounding

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

ARATH:DUS1

NOT|involved_in

GO:0071472: cellular response to salt stress

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

ARATH:DUS1

NOT|involved_in

GO:0070417: cellular response to cold

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

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