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ECOLI:TRAI1

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) traI
Protein Name(s) Multifunctional conjugation protein TraI

DNA relaxase TraI DNA nickase TraI Transesterase TraI DNA helicase I

External Links
UniProt P14565
EMBL M54796
M54796
U01159
AP001918
M29254
X57430
U01159
RefSeq NP_061483.1
WP_000987005.1
PDB 1P4D
2A0I
2L8B
2Q7T
2Q7U
3FLD
PDBsum 1P4D
2A0I
2L8B
2Q7T
2Q7U
3FLD
ProteinModelPortal P14565
SMR P14565
PRIDE P14565
GeneID 1263574
OMA ETHRDAL
EvolutionaryTrace P14565
PRO PR:P14565
Genevestigator P14565
GO GO:0005737
GO:0005524
GO:0003677
GO:0003678
GO:0003917
GO:0046872
GO:0000746
Gene3D 3.40.50.300
InterPro IPR014059
IPR014129
IPR009767
IPR027417
IPR014862
Pfam PF07057
PF08751
SUPFAM SSF52540
TIGRFAMs TIGR02686
TIGR02760

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

Contributes to

GO:0004520

endodeoxyribonuclease activity

PMID:7499339[1]

ECO:0000314

F

See Fig 3 & 5

complete

GO:0004520

endodeoxyribonuclease activity

PMID:15629940[2]

ECO:0000314

F

Fig 4A, TraI is capable of nicking a plasmid containing ORIT.

complete

involved_in

GO:0032508

DNA duplex unwinding

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0003678

P

Seeded From UniProt

complete

involved_in

GO:0000746

conjugation

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009767

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009767

F

Seeded From UniProt

complete

enables

GO:0003678

DNA helicase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009767

F

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009767

F

Seeded From UniProt

complete

enables

GO:0016818

hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009767

F

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

enables

GO:0016853

isomerase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0413

F

Seeded From UniProt

complete

enables

GO:0004386

helicase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0347

F

Seeded From UniProt

complete

enables

GO:0046872

metal ion binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0479

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

involved_in

GO:0000746

conjugation

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0184

P

Seeded From UniProt

complete

enables

GO:0003824

catalytic activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0511

F

Seeded From UniProt

complete

involved_in

GO:0008152

metabolic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0511

P

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0067

F

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Nelson, WC et al. (1995) The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT. J. Biol. Chem. 270 28374-80 PubMed GONUTS page
  2. Matson, SW & Ragonese, H (2005) The F-plasmid TraI protein contains three functional domains required for conjugative DNA strand transfer. J. Bacteriol. 187 697-706 PubMed GONUTS page