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Category:Team Protein Pushers

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesCHICK:S5RDP9Dlnell, Team Protein Pushers2013-11-10 16:08:40 CSTGO:0051607 defense response to virus (P)PMID:24067955ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Decreased viral infection when IFITM3 present in chicken cells. Figure 4 and 5 also.

challenge
requireschangesPSEAE:Q9HZZ3Btilkens, Team Protein Pushers2013-11-10 15:58:40 CSTGO:0016209 antioxidant activity (F)PMID:12485986ECO:0000314 direct assay evidence used in manual assertion

The FOX assay results in Figure 4 demonstrates that Ohr metabolizes hydrogen peroxide.

challenge
requireschangesEBOZM:VP40Lleopold, Team Protein Pushers2013-11-10 15:34:32 CSTGO:0020002 host cell plasma membrane (C)PMID:23297401ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8: Western blots of cells transfected with WT VP40 and several hydrophobic mutants demonstrate that C-terminal domain plasma membrane penetration is critical for viral egress.

challenge
requireschangesHPV16:VE5Lleopold, Team Protein Pushers2013-11-10 14:52:22 CSTGO:0072657 protein localization to membrane (P)PMID:19712955ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows protein localization to the plasma membrane via immunofluresence assay

challenge
unacceptable9PARA:Q14V37Dlnell, Team Protein Pushers2013-11-10 11:57:16 CSTGO:0046718 viral entry into host cell (P)PMID:7853502ECO:0000314 direct assay evidence used in manual assertion

Syncytia only seen in mouse-human hybrid (IL-115) cells when cotransfected with CDV-HA (F protein source not as important, but also required for infection)

challenge
unacceptableFUSCU:A1XCI9Btilkens, Team Protein Pushers2013-10-27 12:32:55 CDTPMID:17584451ECO:0000255 match to sequence model evidence used in manual assertion

Figure 8 shows the hydrophobicity of mature AfpA proteins.

challenge
acceptableHPV16:VE5Lleopold, Team Protein Pushers2013-10-13 10:32:31 CDTGO:0019048 modulation by virus of host morphology or physiology (P)PMID:20686024ECO:0000314 direct assay evidence used in manual assertion

Figure 8 depicts the inhibition of endosome vesicle fusion by HP16 E5 protein

challenge
updatedbyinstructorHPV16:VE5Lleopold, Team Protein Pushers2013-10-13 10:09:56 CDTGO:0020002 host cell plasma membrane (C)PMID:19712955ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows localization to the plasma membrane via immunofluorescence data

challenge
updatedbyinstructorHUMAN:HDAC1Dlnell, Team Protein Pushers2013-10-10 23:37:54 CDTGO:0010629 negative regulation of gene expression (P)PMID:16581785ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3: Acts as a repressor of INF-beta gene expression

challenge
unacceptableHUMAN:HS90BDlnell, Team Protein Pushers2013-10-10 23:02:22 CDTGO:0016032 viral process (P)PMID:24098578ECO:0000314 direct assay evidence used in manual assertion

Figure 3: RD cells transfected with Hsp90-alpha and Hsp90-beta specific siRNA. Only Hsp90-beta siRNA prevented EV71 capsid protein expression showing Hsp90-beta is involved in EV71 infection

challenge
requireschangesCAPHI:C5IZH4Jlensmire, Team Protein Pushers2013-10-07 18:21:48 CDTGO:0006950 response to stress (P)PMID:22110953ECO:0000247 sequence alignment evidence used in manual assertion

Figure 2 shows the predicted amino acid sequence of HSP70 from goat to HSP 70 of cattle

challenge
requireschangesVIBFI:CPDPBtilkens, Team Protein Pushers2013-09-29 11:42:34 CDTGO:0042597 periplasmic space (C)PMID:8393003ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 demonstrates the ability of the mutant strain without the cpdP gene to grow on various media. The inability of the mutant strain to grow on cAMP or cGMP demonstrates the function of the cpdP gene in cAMP metabolism.

challenge
requireschangesNEIG2:B4RKU5Jlensmire, Team Protein Pushers2013-09-29 10:03:23 CDTGO:0006260 DNA replication (P)PMID:21359151ECO:0000247 sequence alignment evidence used in manual assertion

Figure 1 shows the amino acid sequence alignment between SSB of Neisseria gonorrhoeae and SSB in E.coli

challenge
updatedbyinstructorNEIGO:RECAJlensmire, Team Protein Pushers2013-09-29 09:54:19 CDTGO:0016887 ATPase activity (F)PMID:21359151ECO:0000314 direct assay evidence used in manual assertion

Figure 5 measures the amount of ATP hydrolyzed by RecA over time during strand exchange

challenge
unacceptableNEIGO:RECAJlensmire, Team Protein Pushers2013-09-29 09:48:57 CDTGO:0006310 DNA recombination (P)PMID:21359151ECO:0000247 sequence alignment evidence used in manual assertion

Figure 1 shows an amino acid sequence alignment of recA from N. gonorrhoeae aligned to E.coli

challenge
updatedbyinstructorEBOZM:VP40Lleopold, Team Protein Pushers2013-09-25 17:11:46 CDTGO:0044385 integral to membrane of host cell (C)PMID:23297401ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
requireschangesEBOZM:VP40Lleopold, Team Protein Pushers2013-09-25 16:53:47 CDTGO:0019076 viral release from host cell (P)PMID:23297401ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2B, 2C show that when certain key amino acids are mutated (L213A, L295A, V298A) they do not induce the same amount of surface pressure into the plasma membrane as compared to WT

challenge
acceptableSALTI:MGTCJlensmire, Team Protein Pushers2013-09-15 13:21:17 CDTGO:0009405 pathogenesis (P)PMID:19436747ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2. MgtC mutants showed decreased survival in human cells as compared to the WT

challenge
updatedbyinstructorBOMMO:Q5CCJ5Jlensmire, Team Protein Pushers2013-09-15 12:36:31 CDTGO:0008284 positive regulation of cell proliferation (P)PMID:23781494ECO:0000314 direct assay evidence used in manual assertion

Figure 6

challenge
acceptableCAEEL:APN1Jlensmire, Team Protein Pushers2013-09-15 12:17:10 CDTGO:0006284 base-excision repair (P)PMID:22819077ECO:0000314 direct assay evidence used in manual assertion

Figure 10 shows that APN-1 has the ability to repair various DNA lesions

challenge
acceptableCAEEL:APN1Jlensmire, Team Protein Pushers2013-09-15 11:59:26 CDTGO:0017005 3'-tyrosyl-DNA phosphodiesterase activity (F)PMID:22819077ECO:0000314 direct assay evidence used in manual assertion

Figure 10C shows that APN-1 exhibits a 3' repair diesterase activity

challenge
updatedbyinstructorEBOZM:VP40Lleopold, Team Protein Pushers2013-09-14 22:23:23 CDTGO:0046788 egress of virus within host cell (P)PMID:23297401ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8

challenge
unacceptableCAEEL:APN1Jlensmire, Team Protein Pushers2013-09-14 21:18:52 CDTGO:0006284 base-excision repair (P)PMID:22819077ECO:0000247 sequence alignment evidence used in manual assertion

Figure 1 shows C.elegans APN-1 shows homology to Yeast Apn1, which is involved in DNA repair

challenge

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Pages in category "Team Protein Pushers"

The following 5 pages are in this category, out of 5 total.


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