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Category:Team IT

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHUMAN:SYNE2Imumm, Team IT2012-04-14 11:42:49 CDTGO:0005737 cytoplasm (C)PMID:12118075ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that NUANCE can localize to the cytoplasm through immunolabeling.

challenge
acceptableHUMAN:SYNE2Imumm, Team IT2012-04-14 11:40:19 CDTGO:0005634 nucleus (C)PMID:12118075ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that NUANCE localizes to the nucleus through immunolabeling.

challenge
unacceptableHUMAN:KAT5Temini, Team IT2012-04-13 07:19:03 CDTGO:0006950 response to stress (P)PMID:22448206ECO:0000314 direct assay evidence used in manual assertion

Fig 6: Tip 60 shows increase binding to concanavalin A in response to ER stress

challenge
requireschangesARATH:AUX1Imumm, Team IT2012-04-10 20:18:38 CDTGO:0009958 positive gravitropism (P)PMID:10205161ECO:0000269 experimental evidence used in manual assertion

figure 3 shows that AUX1 restores gravitropic root growth.

challenge
requireschangesARATH:AUX1Imumm, Team IT2012-04-10 19:59:23 CDTGO:0048767 root hair elongation (P)PMID:10036773ECO:0000314 direct assay evidence used in manual assertion

figures 4 and 5 show that AUX1 causes root hair development over time through microscopy.

challenge
requireschangesGLOHA:VMHAImumm, Team IT2012-03-27 20:45:45 CDTGO:0072577 endothelial cell apoptosis (P)PMID:14688240ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows endothelial cell apoptosis caused by halysase through fluorescence.

challenge
acceptableSTRPN:ZMPBImumm, Team IT2012-03-27 20:34:05 CDTGO:0009986 cell surface (C)PMID:10792723ECO:0000314 direct assay evidence used in manual assertion

figure 1b shows through immunoelectron microscopy, that ZmpB localizes to the cell surface.

challenge
acceptableHUMAN:DPP2Imumm, Team IT2012-03-27 20:13:07 CDTGO:0031982 vesicle (C)PMID:11067927ECO:0000314 direct assay evidence used in manual assertion

figure 5 shows through confocal microscopy, localization of QPP (DPP2) to the vesicles.

challenge
acceptableYEAST:PAD1Temini, Team IT2012-02-21 20:20:23 CSTGO:0034599 cellular response to oxidative stress (P)PMID:22204397ECO:0000314 direct assay evidence used in manual assertion

Figure 1. assay showing response of pof1 cells to oxidative stress (H202)

challenge
acceptableHUMAN:TOR1ATemini, Team IT2012-02-21 19:33:19 CSTGO:0005783 endoplasmic reticulum (C)PMID:12671990ECO:0000314 direct assay evidence used in manual assertion

Fig 6a-c. Co-localization of torsin A with ER marker PDI. showing localization of torsin A to the endoplasmic reticulum.

challenge
acceptableHUMAN:CRCMImumm, Team IT2012-02-12 17:24:00 CSTGO:0005737 cytoplasm (C)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 1c showed Western Blot Analysis was used to confirm localization of the MCC protein to the cytoplasm.

challenge
acceptableHUMAN:CRCMImumm, Team IT2012-02-12 17:24:00 CSTGO:0005634 nucleus (C)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 1c showed the use of Western Blot Analysis to confirm MCC protein localization in the nucleus of cells.

challenge
requireschangesHUMAN:CRCMImumm, Team IT2012-02-12 17:24:00 CSTGO:0051726 regulation of cell cycle (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 4C and 4D shows that MCC regulates the cell cycle by suppressing the DNA-binding activity of β-catenin/TCF/LEF in the nucleus through Western Blot Analysis. This is also confirmed in the section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels".

challenge
acceptableHUMAN:CRCMImumm, Team IT2012-02-12 17:24:00 CSTGO:0045184 establishment of protein localization (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

The section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels" confirms that MCC relocalizes the β-catenin to the cytoplasm. The results are further confirmed through Western Blot Analysis in Figure 4.

challenge
acceptableHUMAN:DPP10Imumm, Team IT2012-02-12 16:27:24 CSTGO:0090004 positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030ECO:0000314 direct assay evidence used in manual assertion

Figure 1d showed that DPP10 facilitated cell membrane surface expression of Kv4.2 protein through in situ hybridization.

accession #:AY172661

challenge
acceptableMOUSE:LBHImumm, Team IT2012-02-12 16:25:19 CSTGO:0005634 nucleus (C)PMID:11336496ECO:0000314 direct assay evidence used in manual assertion

Section of the text labeled "Biochemical Characterization of Lbh protein" refers to N and C terminus labeling using an antibody to detect localization of the Lbh protein in the nucleus. (figure 2b)

challenge
acceptableSHEEP:UTMPImumm, Team IT2012-02-12 16:22:57 CSTGO:0030414 peptidase inhibitor activity (F)PMID:8942989ECO:0000314 direct assay evidence used in manual assertion

Figure 2.a/b show evidence of %Pepsin activity severely decreased between pH of 2-4.5. Supported text for the experiment is listed under "Inhibitory Activities of Uterine Secretions" with standard assay.

challenge
requireschangesHUMAN:CRCMImumm, Team IT2012-02-12 15:50:37 CSTGO:0030308 negative regulation of cell growth (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Immunofluorescence was used and confirmed in Figure 6A/B to show that MCC is responsible for the suppression of basal cell growth.

challenge
acceptableDROME:Q7KTX7Temini, Team IT2012-02-12 14:01:42 CSTGO:0004842 ubiquitin-protein ligase activity (F)PMID:17456438ECO:0000314 direct assay evidence used in manual assertion

Figure 2

challenge
acceptableMOUSE:AT132Temini, Team IT2012-02-12 14:01:41 CSTGO:0012506 vesicle membrane (C)PMID:22186024ECO:0000314 direct assay evidence used in manual assertion

mouse ATP13A2 is shown to localize to vesicle membranes within the soma and processes of cortical neurons (Fig. 2A)

challenge
acceptableORYSJ:SLR1Imumm, Team IT2012-02-07 19:33:09 CSTGO:0005634 nucleus (C)PMID:10713441ECO:0000314 direct assay evidence used in manual assertion

Results section 3.5 and Figure 5 detail that nucleus localization occurs because of a bipartite NLS in the protein. Epifluorescence microscopy was used to determine the localization pattern.

challenge
acceptableHORVU:IAA1Imumm, Team IT2012-02-07 19:11:38 CSTGO:0015066 alpha-amylase inhibitor activity (F)PMID:2785932ECO:0000314 direct assay evidence used in manual assertion

Table 1

challenge
acceptableRAT:DPP6Imumm, Team IT2012-02-07 19:10:30 CSTGO:0090004 positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030ECO:0000314 direct assay evidence used in manual assertion

figure 1c shows that DPPX (DPP6) promoted a robust trafficking of Kv4.2 to the plasma membrane.

accession #:M76427

challenge

Pages in category "Team IT"

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