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Category:Team GreyJoy

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesMYCTU:ACSAVthompsonui, Team GreyJoy2013-04-11 11:43:43 CDTGO:0006476 protein deacetylation (P)PMID:21627103ECO:0000314 direct assay evidence used in manual assertion

It is shown in figure 3 that ACS is strongly fluorescently labelled after exposure to MsPat with a fluorescent chloracetyl group bound to it, indicating that ACS deacetylated the protein.

challenge
acceptableMYCTU:NPDVthompsonui, Team GreyJoy2013-04-11 11:18:54 CDTGO:0006476 protein deacetylation (P)PMID:21627103ECO:0000314 direct assay evidence used in manual assertion

As shown in figure 5, Acetyl CoA synthetase with a fluorescently marked chloracetyl group was lowered in fluorescent intensity when Rv1151c was added, indicating that Rv1151c deacetylated the protein.

challenge
updatedbyinstructorMYCTU:PATVthompsonui, Team GreyJoy2013-04-11 11:09:55 CDTGO:0016746 transferase activity, transferring acyl groups (F)PMID:21627103ECO:0000314 direct assay evidence used in manual assertion

It is shown in Figure 1B that the protein acetyltransferase binds an acetyl group, and in Figure 3B that the acetyl group is transfered to Acetyl CoA Synthetase.

challenge
updatedbyinstructorMOUSE:SMAD4Vthompsonui, Team GreyJoy2013-04-09 20:41:21 CDTGO:0042733 embryonic digit morphogenesis (P)PMID:23034633ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figures 1 and 2, inactivation of Smad4 leads to a lack of development in the skeletal portion of embryonic digits.

challenge
updatedbyinstructorMOUSE:UPP1Vthompsonui, Team GreyJoy2013-04-09 19:37:04 CDTGO:0006218 uridine catabolic process (P)PMID:23355744ECO:0000314 direct assay evidence used in manual assertion

As shown in figure 1, a mutant of uridine phosphorylase in the liver with markedly higher enzymatic content led to a significant increase in beta alanine content in the liver compared to the wild type.

challenge
acceptableHUMAN:MAOMVthompsonui, Team GreyJoy2013-04-09 19:11:39 CDTGO:1902031 regulation of NADP metabolic process (P)PMID:23334421ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figure 2, the level of NADPH in the cell is comparatively greater in cells with wild type Malic Enzyme 2 than with Malic Enzyme 2 mutants that had no enzymatic activity.

challenge
acceptableHUMAN:MAOXVthompsonui, Team GreyJoy2013-04-09 19:07:29 CDTGO:1902031 regulation of NADP metabolic process (P)PMID:23334421ECO:0000315 mutant phenotype evidence used in manual assertion

In figure 2, it is shown that relative to Malic Enzyme mutants that show no enzymatic activity, wild type Malic Enzyme leads to greater production of NADPH in a cell.

challenge
updatedbyinstructorHUMAN:MAOMVthompsonui, Team GreyJoy2013-03-26 20:06:37 CDTGO:0004470 malic enzyme activity (F)PMID:23334421ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1C.

challenge
updatedbyinstructorHUMAN:MAOXVthompsonui, Team GreyJoy2013-03-26 19:35:17 CDTGO:0004470 malic enzyme activity (F)PMID:23334421ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1C.

challenge
updatedbyinstructorBACSU:EZRAVthompsonui, Team GreyJoy2013-03-05 18:40:14 CSTGO:0051781 positive regulation of cell division (P)PMID:23249255ECO:0000315 mutant phenotype evidence used in manual assertion

As show in Figure 1, a deletion mutation of the gene encoding EzrA leads to improper partitioning of a cell into its daughter cells

challenge
acceptableBACSU:YPBRVthompsonui, Team GreyJoy2013-03-05 18:27:17 CSTGO:0090529 cell septum assembly (P)PMID:23249255ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figure 1, cells with a deletion mutation in the gene for ypbR developed double septa during cytokinesis.

challenge
acceptableECOLI:FTSKVthompsonui, Team GreyJoy2013-02-19 20:28:53 CSTGO:0016887 ATPase activity (F)PMID:20081205ECO:0000314 direct assay evidence used in manual assertion

As indicated in figure 5, ATP is hydrolyzed at a significantly greater rate when FtsK is introduced.

challenge
acceptableECOLI:FTSKVthompsonui, Team GreyJoy2013-02-19 19:46:14 CSTGO:0051301 cell division (P)PMID:21091498ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figure 5, cells with a deletion mutation of varying parts of FtsK form elongate rods, which is indicative of the cell's inability to divide.

challenge
unacceptableMOUSE:BMP2Vthompsonui, Team GreyJoy2013-02-12 18:22:22 CSTGO:0042733 embryonic digit morphogenesis (P)PMID:8682290ECO:0000314 direct assay evidence used in manual assertion

As evidenced in figure 4 by the in situ hybridization, BMP-2 RNA transcripts are present in the terminal ends of the digits as they develop from the forelimb.

challenge
unacceptableMOUSE:SMAD4Vthompsonui, Team GreyJoy2013-02-05 20:14:37 CSTGO:0007398 ectoderm development (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 3 through a direct assay that Smad4 binds to a promoter for Dlx-3, which is known to regulate patterning in the embyronic ectoderm.

challenge
unacceptableMOUSE:SMAD4Vthompsonui, Team GreyJoy2013-02-05 20:11:09 CSTGO:0030509 BMP signaling pathway (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 3 through a direct assay that Smad4 binds to a promoter for the homeodomain Dlx-3 that is responsive to signaling by BMP-2.

challenge
unacceptableMOUSE:SMAD1Vthompsonui, Team GreyJoy2013-02-05 20:06:02 CSTGO:0007398 ectoderm development (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 3 through a direct assay that Smad1 binds to a promoter on the homeodomain Dlx-3 which regulates patterning in the embryonic ectoderm

challenge
unacceptableMOUSE:SMAD1Vthompsonui, Team GreyJoy2013-02-05 20:00:35 CSTGO:0030509 BMP signaling pathway (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 3 through a direct assay that Smad1 binds to an area of the Dlx-3 homeodomain that is responsive to BMP-2 signaling.

challenge
unacceptableMOUSE:BMP2Vthompsonui, Team GreyJoy2013-02-05 19:50:17 CSTGO:2000383 regulation of ectoderm development (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 6 through a direct assay that BMP induces the Dlx-3 homeodomain, which regulates patterning in the embryonic ectoderm.

challenge
correctedthroughchallengesMOUSE:BMP2Vthompsonui, Team GreyJoy2013-02-05 19:47:59 CSTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:11788714ECO:0000314 direct assay evidence used in manual assertion

It is shown by figure 1 through a direct assay that BMP-2 is involved in the induction of Dlx-3 transcription.

challenge
unacceptableHUMAN:MERTKVthompsonui, Team GreyJoy2013-02-05 19:23:55 CSTGO:0071866 negative regulation of apoptotic process in bone marrow (P)PMID:23353780ECO:0000315 mutant phenotype evidence used in manual assertion

It is shown in figure 3 that a knockout mutation in MerTK leads to increased apoptosis rates in response to chemotherapeutic agents.

challenge
acceptableHUMAN:MERTKVthompsonui, Team GreyJoy2013-02-05 19:20:42 CSTGO:2000107 negative regulation of leukocyte apoptotic process (P)PMID:23353780ECO:0000315 mutant phenotype evidence used in manual assertion

It is shown in figure 3 that a knockout mutation in MerTK leads to increased apoptosis rates in response to chemotherapeutic agents.

challenge

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Pages in category "Team GreyJoy"

The following 2 pages are in this category, out of 2 total.


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