GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:Team BICH

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
flaggedPSEAI:A6N5E2JayClark, Team BICH2018-11-20 14:20:29 CSTGO:0099048 CRISPR-cas system (P)PMID:30190308ECO:0000314 direct assay evidence used in manual assertion

Fig. 2B demonstrates that acrIF12, an uncharacterized protein from Pseudomonas aeruginosa, inhibits the effect of DNA modification by type I-F CRISPR, but not against Type I-E. NTR #16423, awaiting response.

challenge
acceptableBPT4:MOTBMckaelaAutumn19, Team BICH2018-11-20 14:17:25 CSTGO:0003690 double-stranded DNA binding (F)PMID:29949907ECO:0000314 direct assay evidence used in manual assertion

Figure 3A shows that Transcription regulatory protein motB/MotB from Bacteriophage T4 binds to host dsDNA and copurifies when the host dsDNA is purified.

challenge
flagged9PSED:A0A1I0UR49JayClark, Team BICH2018-11-20 14:09:31 CSTGO:0099048 CRISPR-cas system (P)PMID:30190308ECO:0000314 direct assay evidence used in manual assertion

Fig. 2B demonstrates that acrIE5, an uncharacterized protein from Pseudomonas otitidis, inhibits the effect of DNA modification by type I-E CRISPR, but not against Type I -F. NTR #16423, awaiting response.

challenge
unacceptableASF:Q5IZK2MckaelaAutumn19, Team BICH2018-11-20 14:06:30 CSTGO:0039660 structural constituent of virion (F)PMID:30185597ECO:0000314 direct assay evidence used in manual assertion

Figure 1 uses a Western Blot to show P72/B646L CDS protein is a structural protein found in African Swine Fever Virus particles.

challenge
updatedbyinstructorASFB7:P10MckaelaAutumn19, Team BICH2018-11-20 14:01:02 CSTGO:0039642 virion nucleoid (C)PMID:30185597ECO:0000314 direct assay evidence used in manual assertion

based on synthesizing figure 1, table 1, and immunogold in figure 3

challenge
acceptableARATH:CRYDMckaelaAutumn19, Team BICH2018-11-20 12:24:49 CSTGO:0000719 photoreactive repair (P)PMID:19074258ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 compares the repair rates of Arabidopsis thaliana in relation to cry3/CRYD/Cryptochrome DASH/ chloroplastic/mitochondrial and its mutant when exposed to light in various amounts after UV treatment.

challenge
acceptableHYPAT:O93963MckaelaAutumn19, Team BICH2018-11-20 11:59:50 CSTGO:0000719 photoreactive repair (P)PMID:17545314ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 compares the survival rates of Trichoderma atroviride in relation to the phr1/DNA photolyase protein and its mutant when exposed to UV light and then either exposed to light or kept in the dark.

challenge
acceptableBPT4:MOTBMckaelaAutumn19, Team BICH2018-11-20 08:26:43 CSTGO:0003697 single-stranded DNA binding (F)PMID:29949907ECO:0000314 direct assay evidence used in manual assertion

Figure 3B shows that Transcription regulatory protein motB/MotB from Bacteriophage T4 binds to host ssDNA and copurifies when the host ssDNA is purified.

challenge
unacceptableHYPAT:O93963MckaelaAutumn19, Team BICH2018-11-19 22:42:51 CSTGO:1904361 positive regulation of spore germination (P)PMID:17545314ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 compares the spore germination of Trichoderma atrovirid in response to light stimulus and exposure to UV radiation of PHR1/DNA photolyase and a mutant of the protein.

challenge
acceptableBOTFB:A0A384JRT3MckaelaAutumn19, Team BICH2018-11-18 10:59:18 CSTGO:0075308 conidium formation (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that BcCRY2 negatively regulates conidiation in Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) by comparing the the conidation transcript levels and condination of wildtype, BcCRY2, and BcCRY2 mutant.

challenge
acceptableBOTFB:A0A384JRT3MckaelaAutumn19, Team BICH2018-11-18 10:50:53 CSTGO:0060258 negative regulation of filamentous growth (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 compares the mycelia growth of Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) wildtype, BcCRY2, and BcCRY2 mutant to show that BcCRY2 negatively regulates radial growth of the fungi.

challenge
acceptableBOTFB:A0A384JIP4MckaelaAutumn19, Team BICH2018-11-18 10:41:25 CSTGO:0000719 photoreactive repair (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 compares the photoreactivation of BcCRY1 to BcCRY2, mutant BcCRY1, and mutant BcCRY2 in photorepair in Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) when exposed to UV light for different intervals.

challenge
unacceptableBPT4:RLIG2MckaelaAutumn19, Team BICH2018-11-18 09:44:31 CSTGO:0003909 DNA ligase activity (F)PMID:21722378ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 compares the ligation products of T4 RNA ligase 2/T4 RNL2 and mutants of this protein in Enterobacteria phage T4.

challenge
unacceptableBPT4:RLIGMckaelaAutumn19, Team BICH2018-11-18 09:38:11 CSTGO:0003909 DNA ligase activity (F)PMID:21722378ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the ligation products of 3'-blocked and 5'- adenylated DNA and T4 RNA ligase 1/T4 RNL1 from Enterobacteria phage T4.

challenge
unacceptableBPT4:RLIGMckaelaAutumn19, Team BICH2018-11-18 09:09:45 CSTGO:0003910 DNA ligase (ATP) activity (F)PMID:26500066ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the catalysis results of T4 RNA ligase 1/T4 RNL1 in combination with varying levels of ATP in Enterobacteria phage T4.

challenge
acceptableHUMAN:SYUAMckaelaAutumn19, Team BICH2018-11-15 15:33:02 CSTGO:0035543 positive regulation of SNARE complex assembly (P)PMID:20798282ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the immunoprecipitation of α-Synuclein/α-S/alpha-Synuclein/Snca of Homo sapiens in transgenic Mus musculus with SNARE complexes immunoprecipitated with SNAP-25 to measure the activity.

challenge
acceptableMOUSE:PO5F1EZN, Team BICH2018-11-13 15:13:30 CSTGO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter (P)PMID:26691508ECO:0000314 direct assay evidence used in manual assertion

In Figure 1, more specifically 1C and 1D, it is shown how POU domain, class 5, transcription factor 1/Transcription Factor Oct4 in Mus musculus upregulates Wdr5 and Dpy30 expression at the RNA level. Figure 6B then shows occupation of the promoter sequence through ChIP assays for the POU domain, class 5, transcription factor 1/Transcription Factor Oct4.

challenge
flagged9GAMM:A0A0U2B983JayClark, Team BICH2018-11-13 14:50:05 CSTGO:0099048 CRISPR-cas system (P)PMID:30190308ECO:0000314 direct assay evidence used in manual assertion

Fig. 2C and E demonstrates that acrVA2.1, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type V-A CRISPR, but not against Type I -C. NTR #16423, awaiting response.

challenge
flagged9GAMM:A0A0U2BNH1JayClark, Team BICH2018-11-13 14:49:13 CSTGO:0099048 CRISPR-cas system (Fig. 2C and E demonstrates that acrIC1, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type I-C CRISPR, but not against Type V-A. NTR #16423, awaiting response.)PMID:30190308IDA: Inferred from Direct Assay
challenge
flagged9GAMM:A0A0U2BNP2JayClark, Team BICH2018-11-13 14:47:35 CSTGO:0099048 CRISPR-cas system (Fig. 2C and E demonstrates that acrVA2, an uncharacterized protein from Moraxella bovoculi, inhibits the effect of DNA modification by type V-A CRISPR, but not against Type I -C. NTR #16423, awaiting response.)PMID:30190308IDA: Inferred from Direct Assay
challenge
acceptableSENDZ:VMckaelaAutumn19, Team BICH2018-11-13 14:39:02 CSTGO:1900226 negative regulation of NLRP3 inflammasome complex assembly (P)PMID:30021903ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows the inhibition of NLRP3 expression levels in cells infected with Sev V protein/Protein V from Sendai virus is present.

challenge
acceptableHENDH:GLYCPMckaelaAutumn19, Team BICH2018-11-13 14:25:41 CSTGO:0044228 host cell surface (C)PMID:19553334ECO:0000314 direct assay evidence used in manual assertion

Figure 3 is showing the trafficking rates of Hendra virus G/glycoprotein G/Hendra G as it makes its way to the surface of the cell in Henipavirus Hendra.

challenge
acceptableHENDH:FUSMckaelaAutumn19, Team BICH2018-11-13 14:22:16 CSTGO:0044228 host cell surface (C)PMID:19553334ECO:0000314 direct assay evidence used in manual assertion

Figure 3 is showing the trafficking rates of Hendra virus F/fusion glycoprotein F0/Hendra F0 as it makes its way to the surface of the cell in Henipavirus Hendra.

challenge
acceptableHBOC2:NP1MckaelaAutumn19, Team BICH2018-11-13 13:55:41 CSTGO:0033120 positive regulation of RNA splicing (P)PMID:26912614ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6D compares the VP mRNA levels of normally functioning non-structural protein NP-1/NP1 and that of a knockout mutant from the human bocavirus using a Northern blot and levels are seen to be decreased with the knockout mutant.

challenge
acceptableHBOC2:NP1MckaelaAutumn19, Team BICH2018-11-13 13:49:42 CSTGO:0031564 transcription antitermination (P)PMID:26912614ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5C shows a Northern Blot that compares VP mRNA in relation to regular NP1/Non-structural protein NP-1 and different mutants and knockout mutants, pCMVNS(NP*)CAP and pCMVNS*(NP*)Cap, in human bocavirus.

challenge
acceptableMOUSE:MYCEZN, Team BICH2018-11-13 13:16:30 CSTGO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter (P)PMID:26691508ECO:0000314 direct assay evidence used in manual assertion

In Figure 1, more specifically 1C and 1D, it is shown how Myc proto-oncogene protein/c-Myc in Mus musculus (Mouse) upregulates Wdr5 and Dpy30 expression at the RNA level. Fig 1H then shows that c-Myc binds to the promoter specifically.

challenge
acceptableBPP21:SPAN1JayClark, Team BICH2018-11-13 13:15:40 CSTGO:0090680 disruption by virus of host outer membrane (P)PMID:22904283ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2c,d,g and h demonstrates that Rz, a spanin inner membrane subunit of the Enterobacteria phage P21 is essential part of the RzRz1 spanin complex, which rapidly lyses the E. coli bacterium. When mutant was observed, the outer membrane was not ruptured, preventing lysis of the bacterium.

challenge
acceptableLAMBD:SPAN2JayClark, Team BICH2018-11-13 13:07:57 CSTGO:0090680 disruption by virus of host outer membrane (P)PMID:22904283ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2a,b,e and f demonstrates that Rz1, a spanin outer lipoprotein subunit of the Escherichia phage lambda is essential part of the RzRz1 spanin complex, which rapidly lyses the E. coli bacterium. When mutant was observed, the outer membrane was not ruptured, preventing lysis of the bacterium.

challenge
acceptableLAMBD:SPAN1JayClark, Team BICH2018-11-13 12:57:44 CSTGO:0090680 disruption by virus of host outer membrane (P)PMID:22904283ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2a,b,e and f demonstrates that Rz, a spanin inner membrane subunit of the Escherichia phage lambda is essential part of the RzRz1 spanin complex, which rapidly lyses the E. coli bacterium. When mutant was observed, the outer membrane was not ruptured, preventing lysis of the bacterium.

challenge
acceptableBPT4:VG31MckaelaAutumn19, Team BICH2018-10-30 14:35:56 CDTGO:0051085 chaperone cofactor-dependent protein refolding (P)PMID:15846365ECO:0000021 physical interaction evidence

Figure 2 shows the interaction of GroEL and Capsid assembly protein Gp31/gp31 within Enterobacteria phage T4/bacteriophage T4 only in the presence of ADP and Mg2+ using mass spectrometry

challenge
unacceptableBPT4:CAPSHMckaelaAutumn19, Team BICH2018-10-30 14:23:24 CDTPMID:15846365ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the folding interaction of GroEL and gp23 within Enterobacteria phage T4/bacteriophage T4 using mass spectrometry.

challenge
acceptable9VIRU:Q27XI9MckaelaAutumn19, Team BICH2018-10-30 13:50:22 CDTGO:0039615 T=1 icosahedral viral capsid (C)PMID:20375175ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows, with cyro-reconstruction that the viral protein 2/VLP 2 has an icosahedral capsid in human bocavirus virus like particles.

challenge
acceptable9CAUD:U5PW93JayClark, Team BICH2018-10-30 13:23:38 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 suggest that gp34 is anTail tubular protein A found in Acinetobacter phage Petty. Protein was identified as part of phage via mass spectroscopy.

challenge
acceptable9CAUD:U5PZN7JayClark, Team BICH2018-10-30 13:22:07 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 suggest that gp36 is an internal virion protein found in Acinetobacter phage Petty. Protein was identified as part of phage via mass spectroscopy.

challenge
acceptable9VIRU:A0A2D0TCG7JayClark, Team BICH2018-10-30 13:19:48 CDTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:30046034ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 demonstrates that acrIIA4 from Listeria monocytogenes prophage is capable of inhibiting the effects of CRISPR-stCas9, but not the effects of spCas9 on sgRNA. Experiment was run on human cells which contained plasmid modifications.

challenge
acceptable9CAUD:A0A2U7VKE8JayClark, Team BICH2018-10-30 13:16:26 CDTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:30046034ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 demonstrates that acrIIA6 protein in Streptococcus phage D1811 is capable of inhibiting the effects of CRISPR-st1Cas9 on sgRNA, but not spCas 9 system. Experiment was run on human cells which contained plasmid modifications.

challenge
flagged9GAMM:A0A0U2BNN7JayClark, Team BICH2018-10-30 13:13:19 CDTGO:0099048 CRISPR-cas system (Fig. 3 demonstrates that the acrVA1 protein effectively prevents various CRISPR Cas12a nucleases, including MbCas12a, Mb3Cas12a, AsCas12a, LbCas12a and FnCas12a from modifying DNA. NTR #16423, waiting for response. These proteins are negatively regulating this process.)PMID:30190308IDA: Inferred from Direct Assay
challenge
flagged9GAMM:A0A0U2BNN7JayClark, Team BICH2018-10-30 13:07:47 CDTGO:0099048 CRISPR-cas system (Fig. 3 demonstrates that acrVA1 from Moraxella bovoculi inhibits the effect of DNA modification by MbCas 12a, LbCas 12, and AsCas12 proteins. Note: NTR #16423 was submitted, waiting for reply. These proteins are negatively regulating this process.)PMID:30190307IDA: Inferred from Direct Assay
challenge
acceptable9CAUD:U5PW88EZN, Team BICH2018-10-29 15:13:21 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp29/Hypothetical protein/uncharacterized protein was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages.

challenge
acceptable9CAUD:U5PVS0EZN, Team BICH2018-10-29 15:11:33 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp27/Hypothetical protein/uncharacterized protein was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages.

challenge
acceptable9CAUD:U5PZF1EZN, Team BICH2018-10-29 15:08:58 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp10/Hypothetical protein/uncharacterized protein was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages.

challenge
acceptable9CAUD:U5PZG8EZN, Team BICH2018-10-29 15:07:12 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp30/Head to tail joining protein was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages.

challenge
unacceptableMOUSE:MYCEZN, Team BICH2018-10-29 10:20:52 CDTGO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific (F)PMID:26691508ECO:0000314 direct assay evidence used in manual assertion

In Figure 1, more specifically 1C, it is shown how Myc proto-oncogene protein/c-Myc in Mus musculus upregulates Wdr5 and Dpy30 expression at the RNA level. With Myc proto-oncogene protein/c-Myc significantly having an impact on how the other transcription factors acted, as shown by Figure 1E. Later in the paper, through the use of this data, it then verifies how direct binding to the promoter sequence caused DNA-binding transcription activator activity.

challenge
acceptable9CAUD:B3FK34EZN, Team BICH2018-10-02 14:40:18 CDTGO:0051649 establishment of localization in cell (P)PMID:28082593ECO:0000314 direct assay evidence used in manual assertion

Figure 1 (C) used Time-Lapse Microscopy, and fluorescence, to show that 201φ2-1p059/PhuZ/phage-encoded tubulin-like cytoskeletal protein localized the GP-105, and coincidentally the Bacteriophage 201φ2-1 DNA within the mid cell of the infected microorganism.

challenge
acceptable9CAUD:B3FK34EZN, Team BICH2018-10-02 14:37:46 CDTGO:0051649 establishment of localization in cell (P)PMID:22726436ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 (D) used Microscopic Single Cell Assay, and fluorescence, to show that 201φ2-1p059/PhuZ/tubulin-like protein localized the plasmid DNA of the organism Bacteriophage 201φ2-1 within the infected bacteria. Figure 6 (H) shows how the phage nucleoid is frequently mispositioned due to the mutant, refortifying the fact that this protein contributes to the localization of the phage nucleoid in the cell.

challenge
unacceptable9MONO:F4YH91MckaelaAutumn19, Team BICH2018-10-02 14:32:56 CDTGO:0005198 structural molecule activity (F)PMID:26196500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows the comparison of the Western blots of Hendra virus nucleocapsid protein/HeV N/nucleocapsid wild type and a mutant in Henipavirus Hendra to show its presence within the virion.

challenge
acceptable9CAUD:A0A2U7VJX7JayClark, Team BICH2018-10-02 14:12:49 CDTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:30046034ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 demonstrates that acrIIA6 protein in Streptococcus phage D1024 is capable of inhibiting the effects of CRISPR-st1Cas9 on sgRNA, but not spCas 9 system. Experiment was run on human cells which contained plasmid modifications.

challenge
unacceptableCAEEL:Q9XX85MckaelaAutumn19, Team BICH2018-10-02 14:07:41 CDTGO:0042048 olfactory behavior (P)PMID:30258230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 compares the chemotaxis in Caenorhabditis elegans between str-217/seven TM receptor wild type and mutants with a C->T mutation and a deletion to show that a mutation and shows a mutation leads to an increase in response compared to wild-type, supporting mutants are DEET resistant.

challenge
unacceptablePRIBE:X2JJ72MckaelaAutumn19, Team BICH2018-10-02 13:18:43 CDTGO:0006582 melanin metabolic process (P)PMID:25143047ECO:0000314 direct assay evidence used in manual assertion

Table 1 suggests that ASIP/agouti receptor plays a part in melanism in Prionailurus bengalensis because it shows the color phenotypes of different variants found in domestic cats, wild cats, and hybrids.

challenge
acceptable9MONO:G5CPX1MckaelaAutumn19, Team BICH2018-09-25 14:34:58 CDTGO:0046813 receptor-mediated virion attachment to host cell (P)PMID:26585033ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the binding of sHeV G/attachment glycoprotein/HeV attachment protein from Heniparvirus Hendra with EFNB2/Fc protein using an ELISA plate.

challenge
acceptable9CAUD:U5PVS5EZN, Team BICH2018-09-18 14:10:15 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp32/Capsid Protein was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages. Also referenced as the major capsid protein.

challenge
acceptableBPT4:SOCJayClark, Team BICH2018-09-18 14:07:50 CDTGO:0098021 viral capsid, decoration (C)PMID:28893988ECO:0000315 mutant phenotype evidence used in manual assertion

Evidence for this the small outer capsid (Soc) protein of bacteriophage T4 comes from the sixth paragraph, “The outer surface of the expanded capsid is decorated by the small outer capsid (Soc) protein...”

Fig. 1 demonstrates where Soc is found on the capsid.

challenge
acceptable9MONO:F4YH96MckaelaAutumn19, Team BICH2018-09-18 14:00:29 CDTGO:0019068 virion assembly (P)PMID:29695428ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 suggests that in Henipavirus Hendra, HeV M/Matrix protein affects VLP (virus like particle) production efficiency because mutants of HeV M protein showed a large decrease in relative VLP levels.

challenge
acceptable9CAUD:U5PZH2EZN, Team BICH2018-09-18 13:52:26 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 used Mass Spectroscopy to show that the gp35/Tail Tubular protein B was present in the Acinetobacter phage Petty, after first being noticed in the genome by homology in other T7-like phages.

challenge
acceptable9CAUD:A0A220GHA5JayClark, Team BICH2018-09-15 20:55:04 CDTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:30046034ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 demonstrates that acrIIA5 is capable of inhibiting the effects of CRISPR-stCas9 and spCas9 on sgRNA. Experiment was run on human cells which contained plasmid modifications.

challenge
acceptable9CAUD:U5PVS9JayClark, Team BICH2018-09-13 11:25:11 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Fig. 5 suggest that gp37 is a structural protein found in Acinetobacter phage Petty. Protein was identified as part of phage via mass spectroscopy.

challenge
acceptable9CAUD:U5PVL2MckaelaAutumn19, Team BICH2018-09-11 14:35:16 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 suggests that Internal core protein/gp 38 is a cellular component of Acinetobacter phage Petty because mass spectrometry shows it is present in the phage.

challenge
acceptable9CAUD:U5PW98EZN, Team BICH2018-09-11 14:12:13 CDTGO:0016787 hydrolase activity (F)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 9 used colorimetric copper-bicinchoninate assay to show that gp39/Dpo1/Tail Fiber Protein from Acinetobacter phage Petty possess hydrolase enzyme activity by producing reducing ends from EPS.

challenge
acceptable9CAUD:U5PW98JayClark, Team BICH2018-09-11 14:10:20 CDTGO:1900232 negative regulation of single-species biofilm formation on inanimate substrate (P)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 10 indicates that gp39/Dpo1 enzyme from acinetobacter phage Petty negatively regulates biofilm formation of acinetobacter calcoaeticus-baumannii strains.

Uniprot name: Tail fiber protein

challenge
unacceptable9CAUD:U5PW98MckaelaAutumn19, Team BICH2018-09-11 14:05:52 CDTGO:0051260 protein homooligomerization (P)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 8 uses mass exclusion chromatography to suggest gp39/Dpo1/tail fiber protein in Acinetobacter phage petty forms a protein hexamer of identical monomers.

challenge

Pages in category "Team BICH"

The following 3 pages are in this category, out of 3 total.


Jump to pages starting with: E J M