Category:Shrodingers Cats

The protein characterized by ORF H240R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein H240R, Ba71V-120

The protein characterized by ORF A137R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Structural protein A137R, GeneBa71V-042

The protein characterized by ORF R298L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Serine/threonine-protein kinase 1, Ba71V-121

The protein characterized by ORF F317L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein F317L, Ba71V-043

The protein characterized by ORF E146L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein E146L, Ba71V-129

The protein characterized by ORF H359L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative DNA-directed RNA polymerase subunit 3 homolog, Ba71V-113

The protein characterized by ORF K421R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein K421R, Ba71V-052

In Table 1 gp303/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp195/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp242/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp048/Hypothetical protein (Uncharacterized protein) was shown to be associated with the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2 - denoted "10".

In Table 1 gp057/HD-domain/PDEase-like protein (Putative HD-domain/PDEase-like protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp198/HNH nuclease domain (Putative HNH endonuclease) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp180/Core domain of the SPFH superfamily (Putative band 7 lipoprotein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp68/Major capsid protein was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.

In Table 1 gp60/DHH phosphoesterase protein (Putative DHH phosphoesterase protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp119/Transcription terminator factor Rho (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp164/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.

In Table 1 gp161/Nicotinate phosphoribosyltransferase (Putative lipoprotein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.

In Table 1 gp111/ Ribonucleoside diphosphate reductase beta subunit (Putative ribonucleoside diphosphate reductase beta subunit) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp137/Putative rIIb-like protein (uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp42/portal protein was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.

The protein characterized by ORF Q706L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative ATP-dependent RNA helicase Q706L, Ba71V-122

The protein characterized by ORF E248R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Protein E248R, Ba71V-132

The protein characterized by ORF K145R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein K145R, Ba71V-051

The protein characterized by ORF I177L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Membrane protein I177L, BA71V-145

The protein characterized by ORF C717R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized Protein C717R, Ba71V-064

The protein characterized by ORF C129R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized Protein C129R, Ba71V-062

The protein characterized by ORF EP1242L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: DNA-directed RNA polymerase subunit 2, Ba71V-053

The protein characterized by ORF CP530R and p15 in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1. This protein is a product of the cleavage of polyprotein 62 and therefore is labelled under it.

Uniprot: Polyprotein pp62, Ba71V-94 p15/p35/p8 proteins from the polypeptide of CP530R (which is pp62)

The protein characterized by ORF CP123L in the African Swine Fever Virus, strain Ba71V was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein CP123L, Ba71V-91

The protein characterized by ORF M1249L in the African Swine Fever Virus, strain Ba71V was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein M1249L, Ba71V-060

The protein characterized by ORF D117L in the African Swine Fever Virus, strain Ba71V, was isolated by SDS-PAGE in figure 1B and noted as p17. It was then identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Major structural protein p17, Ba71V-107

The protein characterized by ORF NP1450L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Probable DNA-directed RNA polymerase subunit 1 homolog, Ba71V-99

The protein characterized by ORF D1133L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative ATP-dependent RNA helicase D1133L, Ba71V-106

Table 1 shows that ORFEP402R (uniprot: CD2 homolog) was found to be a component of African Swine Fever Virus strain Ba71V by liquid chromatography tandem mass spectroscopy.

The protein characterized by ORF A104R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry as shown in table 1.

Uniprot: BA71V-033, Viral histone-like protein

Table 1 shows that ORFE120R Protein pE120R (uniprot: Structural protein p14.5) was found to be a component of African Swine Fever Virus strain Ba71V by liquid chromatography tandem mass spectroscopy.

The protein characterized by ORF B962L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative RNA helicase B962L, Ba71V-072

Table 1 shows that ORFB119L FAD-Linked Sulfhydryl Oxidase was found to be a component of African Swine Fever Virus strain Ba71V by liquid chromatography tandem mass spectroscopy.

Figure 8 shows through electron microscopy that the expression of polyprotein pp62 in the African Swine Fever Virus allows for the assembly of shell-like membrane, when it is joined with the presence of polyprotein pp220. Figure 8 also shows the interaction of the two polyproteins to form this shell through the use of gold-labelling.

Uniprot: Polyprotein pp62, Ba71V-94

Figure 8 shows through electron microscopy that the expression of polyprotein pp220 in the African Swine Fever Virus allows for the assembly of coating on the membrane. This can be seen to lead to the formation of a viral core-shell when joined with another polyprotein, pp62.

Uniprot: Polyprotein pp220, Ba71V-92

In Table 1 gp58/ tail protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is identified as phage tail samples.

In Table 1 gp57/tape measure protein/lysin (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is identified as phage tail samples.

In Table 1 gp53/putative tail tube protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 3, of Figure 1D, which is identified as phage tail samples.

In Table 1 gp52/major tail sheath protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 3, of Figure 1D, which is identified as phage tail samples.

In Table 1 gp49/phage tail component (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is phage tail samples.

Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Uncharacterized protein ZF40_0060 (gp60) is a component of Pectobacteriumphage ZF40 with a molecular weight of 34.25 kDa. Figure 1C shows that Uncharacterized protein ZF0_0060 was isolated using SDS-PAGE in lane 2.

Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative baseplate protein ZF40_0061 (gp61) is a component of Pectobacteriumphage ZF40 with a molecular weight of 24.51 kDa. Figure 1C shows that Putative baseplate protein ZF0_0061 was isolated using SDS-PAGE in lane 2.

Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Uncharacterized protein ZF40_0062 (gp62) is a component of Pectobacteriumphage ZF40 with a molecular weight of 15.02 kDa. Figure 1C shows that Uncharacterized protein ZF0_0062 was isolated using SDS-PAGE in lane 2.

Table 1 shows that protein gp48 is a component of the Pectobacterium phage ZF40 with a molecular weight of 18.74 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 displays the isolation of the protein using SDS-PAGE.

Uniprot Name(s): Uncharacterized Protein, ZF40_0048

Table 1 shows that Elecrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative tail-fiber protein ZF40_0067 (gp67) is a component of Pectobacteriumphage ZF40 with a molecular weight of 71.51 kDa. Figure 1C shows that Putative tail fiber protein ZF40_0067 was isolated by SDS-PAGE in lane 2.

Table 1 shows that Elecrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative baseplate protein ZF40_0063 (gp63) is a component of Pectobacteriumphage ZF40 with a molecular weight of 42.89 kDa. Figure 1C shows that Putative baseplate protein ZF0_0063 was isolated using SDS-PAGE in lane 2.

Table 1 shows that protein gp46 is a major capsid protein in the Pectobacterium phage ZF40 with a molecular weight of 34.35 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 shows that this protein was isolated through SDS-PAGE.

Uniprot Name(s): Putative major capsid protein, ZF40_0046

Table 1 shows that protein gp44 is a capsid component of the Pectobacterium phage ZF40 with a molecular weight of 44.12 kDa as determined through ESI-tandem mass spectrometry. In figure 1D lane 3, it is shown that the protein was isolated through SDS-PAGE.

Uniprot: ZF40_0044, Putative head protein

Table 1 shows that protein gp43 is a component of Pectobacterium phage ZF40 with a molecular weight of 36.01 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-Page.

Uniprot: ZF40_0043, Putative head protein

Table 1 shows that protein gp42 is a capsid component in Pectobacterium phage ZF40 with a molecular weight of 51.92 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-PAGE.

Uniprot: ZF40_0042, Putative portal protein

In figure 6, the image shows that gp131 of Pseudomonas phage ϕKZ is a component of the base plate of the virion through immune-gold labeling.

Uniprot: PHIKZ131

In Table 1 gp273/Conserved phage protein (uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp123/T7-like Pol I DNA Polymerase (putative T7-like Pol I DNA Polymerase) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1.

In Table 1 gp318/terminase large subunit (Intein-containing putative terminase large subunit) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

In Table 1 gp101/tail fiber protein (putative tail protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

Figure 1B & 1C show increased expression and production of pyoverdine in Pseudomonas aeruginosa, the final product of the biosynthetic pathway that pvdA/L-ornithine N(5)-monooxygenase is involved in, when iron levels are low, denoting upregulation of pvdA/L-ornithine N(5)-monooxygenase under iron-deficient conditions.

Figure 1B & 1C show increased expression and production of pyoverdine in Pseudomonas aeruginosa, the final product of the biosynthetic pathway that pvdA/L-ornithine N(5)-monooxygenase is involved in, when iron levels are low.

Uncharacterized Protein/47 was found to be a virion component of Salmonella Phage SPN3US using Electrospray Ionization Mass Spectrometry and is listed on Table 4.

Figure 3D shows Electrophoretic Mobility Shift Assays that depict the binding of master quorum sensing transcription factor LuxR to the LuxC promoter region of Vibrio harveyi

Should have GenBank:X07084.1 in the with/from field

Updated GO term: see Discussion page

Figure 1, lane 4 shows that gene ORF8, which codes for Envelope glycoprotein B, is present in Kaposi’s Sarcoma-associated Herpesvirus virion.

In Table 1 gp270/putative-lectin like domain protein (an uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.

Figure 5 displays the phosphotransfer profiling of Caulobacter crescentus and shows that the response regulator pleD is selectively phosphorylated by the histidine kinase pleC.

In figure 5, phosphotransfer profiling demonstrates phosphorylation of PleD from PleC, a non-motile phage-resistant protein, in Caulobacter crescentus.

Figures 5 shows that Non-motile and phage-resistance protein (PleC), in Caulobacter crescentus, demonstrates phosphorylation of PleD, its vivo target.