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Category:MichSt14A 23

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorPSEAI:P95429Vandecap, MichSt14A 232014-04-06 15:38:51 CDTGO:0045892 negative regulation of transcription, DNA-templated (P)PMID:23279839ECO:0000314 direct assay evidence used in manual assertion

Figure 1 A & B shows the diminished transcript levels in the presence of ExsD compared to ExsA, additionally it shows the decline of transcript levels in the presence of increased concentrations of ExsD.

challenge
unacceptableXANCP:Q8PB94Vandecap, MichSt14A 232014-04-06 12:29:48 CDTGO:0009306 protein secretion (P)PMID:23777429ECO:0000314 direct assay evidence used in manual assertion

Figure 5 demonstrates the analysis of amino acid residues of the FHIPEP motif to protein–protein interactions. When HrcVR138A-c-Myc and HrcV

D161A-c-Myc were incubated with GST or GST fusion proteins, both coeluted with GST-HpaC, GST-HrpE, and GST fusions of T3S effectors but were not detected in the eluates of GST-HrcV324-645, GST-HrcN, GST-HrcU255-357, GST- HpaB, and GST-HrpB2. The absence of detectable interactions of HrcVR138A-c-Myc and HrcVD161A-c-Myc with HpaB, HrpB2, and T3S system components explains the loss-of-function phenotypes and implies that these interactions are needed for protein function.

challenge
unacceptableHUMAN:FAF1Vandecap, MichSt14A 232014-04-03 13:26:55 CDTGO:0008219 cell death (P)PMID:22876279ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 B & C shows the pro-apoptosis abilities of hFAF1. The plates show that in those cells where hFAF1 was knocked out there was a significant increase in cell growth, in contrast those plates where the hFAF1 was left functioning properly there was a lack of cell growth, which was due to hFAF1 ability to break down HSP 70. This is again shown in the table, which specifically points out the amount of colonies.

challenge
unacceptableYERE1:E7BAY2Vandecap, MichSt14A 232014-04-03 11:25:46 CDTGO:0004725 protein tyrosine phosphatase activity (F)PMID:24658611ECO:0000314 direct assay evidence used in manual assertion

Figure 5 demonstrates LcrQ ability to negatively regulate gene expression because the graph shows limited B-galactosidase activity for LcrQ, while there is an up regulation of B-glactosidase activity for the LcrF gene that is responsible for positive gene expression.

challenge
updatedbyinstructorPSEAE:EXSAVandecap, MichSt14A 232014-04-03 11:21:23 CDTGO:2000144 positive regulation of DNA-templated transcription, initiation (P)PMID:24142246ECO:0000314 direct assay evidence used in manual assertion

Figure 8 shows that the binding site is required for transcription activation, but the paper already established activation via IDA in Fig 7

challenge
requireschangesYERE1:E7BAZ6Vandecap, MichSt14A 232014-04-03 11:19:50 CDTGO:2000144 positive regulation of DNA-templated transcription, initiation (P)PMID:24142246ECO:0000314 direct assay evidence used in manual assertion

Figure 1

challenge
updatedbyinstructorHUMAN:FAF1Vandecap, MichSt14A 232014-04-03 10:29:30 CDTGO:0010942 positive regulation of cell death (P)PMID:15596450ECO:0000314 direct assay evidence used in manual assertion

Figure 9 demonstrates hFAF1 ability to induce apoptosis, specifically through heat shock. The graph in figure 9 shows that HSP+ hFAF1 had the lowest cell viability of all samples proposed and cell viability was confirmed through 3-2, 5-diphenyltetrazolium bromide assay.

challenge

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