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Category:Ca$h Money Curators

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptable9CAUD:H6X3M5FFitzgerald, Ca$h Money Curators2019-04-14 22:07:00 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp068w(ORF068), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 56.144 (KDa).

Uniprot: Uncharacterized protein, RaK2_00068

challenge
acceptable9CAUD:H6X3M2FFitzgerald, Ca$h Money Curators2019-04-14 22:01:17 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp065(ORF065), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 45.317 (KDa).

Uniprot: Uncharacterized protein, RaK2_00065

challenge
unacceptableYEAST:FDC1Navick, Ca$h Money Curators2019-04-11 21:35:48 CDTGO:0006113 fermentation (P)PMID:30625138ECO:0007680 chromatography evidence used in manual assertion

Figure 4C shows that Fdc1 (Ferulic acid decarboxylase 1) positively regulates the production of 4VG in S. eubayanus.

challenge
unacceptableYEAST:FDC1Navick, Ca$h Money Curators2019-04-11 21:35:47 CDTGO:0006113 fermentation (P)PMID:30625138ECO:0007680 chromatography evidence used in manual assertion

Figure 4C shows that Fdc1 (Ferulic acid decarboxylase 1) positively regulates the production of 4VG in S. cerevisiae.

challenge
unacceptableYEAST:FDC1Navick, Ca$h Money Curators2019-04-11 21:35:47 CDTGO:1901468 positive regulation of ferulate catabolic process (P)PMID:30625138ECO:0001258 spectrophotometry evidence used in manual assertion

Figures 2 and 3 shows that Fdc1 (Ferulic acid decarboxylase 1) positively regulates the decarboxylation of ferulic acid in S. eubayanus.

challenge
updatedbyinstructorYEAST:FDC1Navick, Ca$h Money Curators2019-04-11 21:35:46 CDTGO:1901067 ferulate catabolic process (P)PMID:30625138ECO:0001258 spectrophotometry evidence used in manual assertion

Figures 2 and 3 shows that Fdc1 (Ferulic acid decarboxylase 1) positively regulates the decarboxylation of ferulic acid in S. cerevisiae.

challenge
acceptable9CAUD:H6X3L7FFitzgerald, Ca$h Money Curators2019-04-09 16:28:57 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp060w(ORF060w), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 56.225(KDa).

Uniprot: Uncharacterized protein, RaK2_00060

challenge
acceptable9CAUD:H6X3L8FFitzgerald, Ca$h Money Curators2019-04-09 16:27:31 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp061w(ORF61w), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 12.577(KDa).

Uniprot: Uncharacterized protein, RaK2_00061

challenge
acceptable9CAUD:H6X4V0Navick, Ca$h Money Curators2019-04-09 16:16:05 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp493 (493, orf493, uncharacterized protein, RaK2 00493) has a molecular weight of 21.475 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X482Navick, Ca$h Money Curators2019-04-09 16:14:34 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp275 (275, orf275, uncharacterized protein, RaK2 00275) has a molecular weight of 64.095 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X440Navick, Ca$h Money Curators2019-04-09 16:12:26 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp233 (233, orf233, uncharacterized protein, RaK2 00233) has a molecular weight of 30.971 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3Y2Navick, Ca$h Money Curators2019-04-09 16:09:52 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp175 (175, orf175, uncharacterized protein, RaK2 00175) has a molecular weight of 21.079 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3Y2Navick, Ca$h Money Curators2019-04-09 16:09:52 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp175 (175, orf175, uncharacterized protein, RaK2 00175) has a molecular weight of 21.079 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3W7Navick, Ca$h Money Curators2019-04-09 16:05:28 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp160 (160, orf160 uncharacterized protein, RaK2 00160) has a molecular weight of 87.718 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3W3Navick, Ca$h Money Curators2019-04-09 16:03:30 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp156 (156, orf156, uncharacterized protein, RaK2 00156) has a molecular weight of 34.198 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3V8Navick, Ca$h Money Curators2019-04-09 16:01:33 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp151(151, orf151, uncharacterized protein, RaK2 00151) has a molecular weight of 20.561 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3P8Navick, Ca$h Money Curators2019-04-09 16:00:01 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp091 (091, orf091, uncharacterized protein, RaK2 00091) has a molecular weight of 28.209 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3N8Navick, Ca$h Money Curators2019-04-09 15:58:06 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp081w (081, orf081w, uncharacterized protein, RaK2 00081) has a molecular weight of 27.44 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X3N6Navick, Ca$h Money Curators2019-04-09 15:55:43 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp079w (079, orf079w, uncharacterized protein, RaK2 00079) has a molecular weight of 87.857 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
updatedbyinstructor9CAUD:A0A2P1CKN7FFitzgerald, Ca$h Money Curators2019-04-09 15:47:11 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The major tail protein (gp15, ORF15) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 33.329(KDa).

challenge
updatedbyinstructor9CAUD:A0A2P1CKH4FFitzgerald, Ca$h Money Curators2019-04-09 15:43:55 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The major capsid protein (gp06, ORF06) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 39.892 (KDa)

challenge
acceptable9CAUD:H6X3Y9JayClark, Ca$h Money Curators2019-04-09 15:39:43 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp182 (ORF182), putative structural protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 182 has a molecular mass of 13.142 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X4V3JayClark, Ca$h Money Curators2019-04-09 15:35:02 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp496 (ORF496), putative structural protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 496 has a molecular mass of 22.787 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3V2JayClark, Ca$h Money Curators2019-04-09 15:27:20 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp145w (ORF145w), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 145w has a molecular mass of 25.061 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X4W3JayClark, Ca$h Money Curators2019-04-09 15:17:50 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 show that gp506 (ORF 506), hypothetical protein, has a molecular mass of 53.675 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3P6Rodrigzma3, Ca$h Money Curators2019-04-09 12:32:21 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp089 (ORF089), a portal vertex protein, produces a residue via SDS PAGE. Table S3 determines that gp089 has a molecular mass of 64.744 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
updatedbyinstructor9CAUD:A0A2P1CKJ9Rodrigzma3, Ca$h Money Curators2019-04-09 12:25:32 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

Page 583 shows that gp11 (ORF11), a head-tail adaptor, was identified by LC-MS/MS based proteomics as present in the virion of Pantoea Bacteriophage vB_PagS_Vid5. Table S3 shows that gp11 possesses a molecular mass of 18.734 kDa.

challenge
acceptable9CAUD:H6X3P9Rodrigzma3, Ca$h Money Curators2019-04-09 12:22:13 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp92 (ORF92), a prohead core scaffolding protein and protease, produces a residue via SDS PAGE. Table S3 determines that gp92 has a molecular mass of 22.870 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3S0Rodrigzma3, Ca$h Money Curators2019-04-09 00:03:02 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp113 (ORF113), a putative single-stranded binding protein, produces a residue via SDS PAGE. Table S3 determines that gp113 has a molecular mass of 40.488 kDa. Cellular components were determined via MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
updatedbyinstructor9CAUD:A0A2P1CKQ2FFitzgerald, Ca$h Money Curators2019-03-26 16:21:33 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The tail fiber protein (gp25, ORF25) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 44.464(KDa).

challenge
acceptable9CAUD:I1TE42Navick, Ca$h Money Curators2019-03-26 16:19:53 CDTGO:0019012 virion (C)PMID:22514640ECO:0005527 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion

Table 3 and Figure 5 say that ORF142 (Pore-forming tail tip protein) has a molecular weight of 121.1 kDa. The paper mentions the difference between this and the Uniprot value of 132.3 likely comes from post-translational processing of the protein.

Organism_uniprot:Escherichia virus AKFV33

Organism_paper:vB_EcoS_AKFV33

challenge
acceptable9CAUD:I1TE46Navick, Ca$h Money Curators2019-03-26 16:17:46 CDTGO:0019012 virion (C)PMID:22514640ECO:0005527 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion

Table 3 and Figure 5 say that ORF146 (Major Tail Protein) has a molecular weight of 51.7 kDa.

Organism_uniprot:Escherichia virus AKFV33

Organism_paper:vB_EcoS_AKFV33

challenge
acceptable9CAUD:I1TE37Navick, Ca$h Money Curators2019-03-26 16:16:23 CDTGO:0019012 virion (C)PMID:22514640ECO:0005527 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion

Table 3 and Figure 5 say that ORF137 (Tail Fiber Protein) has a molecular weight of 95.8 kDa.

Organism_uniprot:Escherichia virus AKFV33

Organism_paper:vB_EcoS_AKFV33

challenge
acceptable9CAUD:I1TE50Navick, Ca$h Money Curators2019-03-26 16:11:35 CDTGO:0019012 virion (C)PMID:22514640ECO:0005527 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion

Table 3 and Figure 5 say that ORF150 (Major Head Protein) has a molecular weight of 32.6 kDa. The paper mentions the difference between this and the Uniprot value of 50.6 likely comes from post-translational processing of the protein.

Organism_uniprot: Escherichia virus AKFV33

Organism_paper: vB_EcoS_AKFV33

challenge
acceptable9CAUD:H6X3R2FFitzgerald, Ca$h Money Curators2019-03-25 20:57:12 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp105(ORF105), a putative tail protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 27.854.

challenge
acceptable9CAUD:H6X3R3FFitzgerald, Ca$h Money Curators2019-03-25 20:55:10 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp106(ORF106w), a putative tail protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 24.651 through bioinformatics.

challenge
acceptable9CAUD:H6X3U2FFitzgerald, Ca$h Money Curators2019-03-25 20:53:22 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp135(ORF135), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 23.259.

challenge
acceptable9CAUD:H6X3R4FFitzgerald, Ca$h Money Curators2019-03-25 20:46:56 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp107(ORF107), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 21.133.

challenge
acceptable9CAUD:H6X4Z1FFitzgerald, Ca$h Money Curators2019-03-19 16:15:35 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp534(ORF534) produces a protein residue via SDS-PAGE. Table S3 states that gp534 is a putative virion protein with a molecular weight of 70.781 due to bioinformatics.

Organism: Klebsiella Phage vB_KleM-Rak2

challenge
acceptable9CAUD:H6X3Q1JayClark, Ca$h Money Curators2019-03-19 16:02:37 CDTGO:0098017 viral capsid, major subunit (C)PMID:23593293ECO:0000318 biological aspect of ancestor evidence used in manual assertion

Fig. 7 shows that gp 094, major capsid protein, of Klebsiella Phage vB_KleM-RaK2, shows phylogenetic similarity to Enterobacteria phage T4 major capsid protein gp23.

challenge
unacceptable9CAUD:H6X3Q1JayClark, Ca$h Money Curators2019-03-19 15:27:37 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp 094, major capsid protein, of Klebsiella Phage vB_KleM-RaK2, produces a protein residue. Table S3 determines that gp 094 has a molecular mass of 42.725 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X478FFitzgerald, Ca$h Money Curators2019-03-19 15:20:32 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through Mass spectrometry, Figure 5 identifies the protein gp 531w(ORF531w), a structural protein, via SDS-PAGE. It is defined as a putative tail protein through bioinformatics in table S3 with a molecular weight of 97.727. Gp531w is a structural protein of Klebsiella Phage vB_KleM-RaK.

challenge
acceptable9CAUD:H6X4Y7FFitzgerald, Ca$h Money Curators2019-03-19 15:13:54 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 5 identifies the protein gp530w through Mass spectrometry and it is defined as a putative tail protein through bioinformatics in table S3. Gp530w is a structural protein of Klebsiella Phage vB_KleM-RaK.

challenge
acceptable9CAUD:H6X4Y4FFitzgerald, Ca$h Money Curators2019-03-19 15:08:37 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through MS/MS, shown in figure 5, it is determined that gp527w(ORF527w) is present in mature virus particles via SDS-PAGE. Through bioinformatics, shown in Table S3, the protein was determined to be a putative tail fiber protein with a molecular weight of 79.449 in Klebsiella Phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X4Y3FFitzgerald, Ca$h Money Curators2019-03-19 15:03:41 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through MS/MS, Figure 5 shows that gp526w(ORF526w), a putative tail protein, is a protein residue via SDS-PAGE. Through bioinformatics, shown in Table S3, the protein was determined to be a putative tail protein and has a molecular weight of 63.021.

Organism: Klebsiella PhagevB_KleM-Rak2.

challenge
acceptable9CAUD:A0A2P1CL82Rodrigzma3, Ca$h Money Curators2019-03-19 15:03:40 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Page 583 shows that gp10 (ORF10), a putative EPS depolymerase enzyme, was identified by LC-MS/MS based proteomics as present in the virion of Pantoea Bacteriophage vB_PagS_Vid5. Table S3 shows gp10 to possess a molecular mass of 95.823 kDa.

challenge
acceptable9CAUD:H6X3M9Navick, Ca$h Money Curators2019-03-19 14:37:38 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp072 (072, orf072, uncharacterized protein, RaK2 00072) has a molecular weight of 15.024 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X4Y5Navick, Ca$h Money Curators2019-03-19 14:35:39 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp528w (528, orf528, uncharacterized protein, RaK2 00528) has a molecular weight of 121.755 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
updatedbyinstructor9CAUD:A0A2P1CL06Navick, Ca$h Money Curators2019-03-19 14:32:25 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3: Liquid Chromatography and Mass Spectroscopy indicate that ORF99 (gp99, Lysis regulatory protein, Vid5_gp99) is present in the organism Pantoea phage vB_PagS_Vid5.

challenge
updatedbyinstructor9CAUD:A0A2P1CKR0Navick, Ca$h Money Curators2019-03-19 14:29:09 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 Liquid Chromatography and Mass Spectroscopy indicate that ORF13 (gp13, Neck Protein, Vid5_gp13) is present in the organism Pantoea phage vB_PagS_Vid5.

challenge
updatedbyinstructor9CAUD:A0A2P1CKJ3Rodrigzma3, Ca$h Money Curators2019-03-19 14:28:24 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3: Liquid chromatography mass spectrometry evidence identified gp18 (ORF18), a protein of Pantoea Bacteriophage vB_PagS_Vid5, as a component of the virion.

challenge
updatedbyinstructor9CAUD:A0A2P1CKP5Navick, Ca$h Money Curators2019-03-19 14:27:06 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3: Liquid Chromatography and Mass Spectroscopy indicate that ORF07 (gp7, Uncharacterized Protein, Vid5_gp07) is present in the organism Pantoea phage vB_PagS_Vid5.

challenge
acceptable9CAUD:A0A2P1CKJ4JayClark, Ca$h Money Curators2019-03-19 14:25:34 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Pg. 583 shows that the tail fiber protein (gp22, ORF22) was found in Pantoea phage vB_PagS_Vid5. LC MS/MS was run on the protein, and a molecular weight of 98.324 kDa, with 7 amino acid residues was recorded. Supplementary Table 3 shows results of mass spectrometry.

challenge
acceptable9CAUD:A0A2P1CKP9JayClark, Ca$h Money Curators2019-03-19 14:18:25 CDTGO:0019012 virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertion

Pg. 583 shows that the portal protein (gp03, ORF03) was found in Pantoea phage vB_PagS_Vid5. LC MS/MS was run on protein, and a molecular weight of 54.818 kDa, with 48 amino acid residues was recorded. Supplementary Table 3 shows results of mass spectrometry.

challenge
updatedbyinstructor9CAUD:H6X3K8Rodrigzma3, Ca$h Money Curators2019-02-26 16:23:35 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 5 and Table S3 show that gp 051 (ORF 051), a conserved phage protein, of Klebsiella Phage vB_KleM-RaK2, produces a protein residue. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

challenge
acceptable9CAUD:H6X3K6JayClark, Ca$h Money Curators2019-02-26 16:21:15 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp049 (ORF 049), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 049 has a molecular mass of 34.878 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3J5Rodrigzma3, Ca$h Money Curators2019-02-26 16:19:04 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 5 shows that gp 038 (ORF 038), a neck protein, of Klebsiella Phage vB_KleM-RaK2, produces a protein residue. Table S3 determines that gp 038 has a molecular mass of 30.294 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

challenge
acceptable9CAUD:H6X3K5JayClark, Ca$h Money Curators2019-02-26 16:15:33 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp048 (ORF 048), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 048 has a molecular mass of 20.374 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3K4JayClark, Ca$h Money Curators2019-02-26 16:09:35 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp047 (ORF 047), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 047 has a molecular mass of 23.356 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3G8Rodrigzma3, Ca$h Money Curators2019-02-26 16:06:52 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 5 shows that gp 011 (ORF 011), a putative virion protein, of Klebsiella Phage vB_KleM-RaK2, produces a protein residue. Table S3 determines that gp 011 has a molecular mass of 12.065 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

challenge
acceptable9CAUD:H6X3K0JayClark, Ca$h Money Curators2019-02-26 16:05:00 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp043 (ORF 043), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 043 has a molecular mass of 28.720 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3J9JayClark, Ca$h Money Curators2019-02-26 16:01:37 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp042 (ORF 042), uncharacterized protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 042 has a molecular mass of 24.950 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3G7JayClark, Ca$h Money Curators2019-02-26 15:54:16 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp010 (ORF 010), Putative structural protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 010 has a molecular mass of 13.932 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:A0A220GHA5FFitzgerald, Ca$h Money Curators2019-02-12 16:12:27 CSTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:28785032ECO:0007040 plaque assay evidence used in manual assertion

Figure 2b shows how the activity of an Anti-CRISPR protein (acrIIA5) with A CR1 immune cell in a Streptococcus phage (D4276) can increase sensitivity to cos-type phage D5842

challenge
acceptable9CAUD:H6X3K7FFitzgerald, Ca$h Money Curators2019-02-12 16:03:40 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through Mass Spectroscopy, Table S3 states that gp050 (Gene Rak2_00050) proposed function in Enterobacteria Phage vB_KleM-Rak2 is identified as a Conserved Phage protein with a Molecular Weight of 33.576.

challenge
acceptable9CAUD:H6X3Q5Navick, Ca$h Money Curators2019-02-12 16:01:10 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 and Figure 5 say that gp098 (098, orf098, uncharacterized protein, RaK2 00098) has a molecular weight of 62.869 kDa in Enterobacteria phage vB_KleM-RaK2.

challenge
updatedbyinstructor9CAUD:H6X3M8Rodrigzma3, Ca$h Money Curators2019-02-12 15:53:36 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 shows that gp071 has a molecular mass of 131.63 kDa and cellular components were determined via bioinformatics and MS/MS-based proteomics. This protein is not specifically shown in figure 5.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X3J8Rodrigzma3, Ca$h Money Curators2019-02-12 15:43:39 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table S3 shows that gp041 has a molecular mass of 97.19 kDa and cellular components were determined via bioinformatics and MS/MS-based proteomics. Protein was not shown in fig. 5.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
acceptable9CAUD:H6X4Y9JayClark, Ca$h Money Curators2019-02-12 15:34:17 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp532 (ORF 532), Putative structural protein, produces a protein residue via SDS PAGE. Table S3 determines that gp 532 has a molecular mass of 87.315 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

Organism: Klebsiella Phage vB_KleM-RaK2

challenge
updatedbyinstructor9CAUD:H6X3Q1JayClark, Ca$h Money Curators2019-02-12 15:08:01 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig. 5 shows that gp 094 (ORF 094), major capsid protein, of Klebsiella Phage vB_KleM-RaK2, produces a protein residue. Table S3 determines that gp 094 has a molecular mass of 42.725 kDa. Cellular components were determined via bioinformatics and MS/MS -based proteomics.

challenge
acceptableLAMBD:FIBERJayClark, Ca$h Money Curators2019-02-05 15:26:56 CSTGO:0019062 virion attachment to host cell (P)PMID:1439823ECO:0007040 plaque assay evidence used in manual assertion

Fig. 4 demonstrates that Ur-lambda, which contains the tail fiber protein (stf), adsorbed to E. coli substantially faster than wild type lambda, which did not contain tail fiber proteins. Ur-lambda was capable of adsorbing in the presence of glucose, while wild type lambda was only capable of adsorbing in the presence of maltose. This evidence shows that the tail fiber proteins are involved in adsorption to the host cell.

Organism: Escherichia phage lambda

challenge
acceptableLAMBD:FIBERJayClark, Ca$h Money Curators2019-02-05 14:47:42 CSTGO:0098024 virus tail, fiber (C)PMID:1439823ECO:0007044 transmission electron microscopy evidence used in manual assertion

Fig. 3 shows that gene product stf produces a band on an SDS gel at approximately 78 kDa. Fig. 1 shows an electron microscopy image of Ur - lambda. Tail fibers are visible on the phage tail. The combine evidence shows that the stf gene product results in tail fiber formation at the virion tail.

Organism: Escherichia phage lambda

challenge
acceptableCAUVC:Q9AAR6FFitzgerald, Ca$h Money Curators2019-01-29 16:03:36 CSTGO:0000160 phosphorelay signal transduction system (P)PMID:16176121ECO:0001202 in vitro protein kinase assay evidence used in manual assertion

Fig 5 showed phosphorylation of the kinase CC0530 (CenK) to reveal the regulator CC3743 (CenR)

Organism:Caulobacter Crescentus

challenge
acceptableCAUVC:Q9AAR6JayClark, Ca$h Money Curators2019-01-29 15:57:30 CSTGO:0000155 phosphorelay sensor kinase activity (F)PMID:16176121ECO:0001202 in vitro protein kinase assay evidence used in manual assertion

Fig. 5 shows that the cell envelope kinase (CenK, CC0530) strongly preferred phosphorylating in the presence of CenR (CC03743). This was accomplished using phosphotransfer profiling; comparing the activity of CenK and CenR relative to other substrates.

Organism: Caulobacter crescentus

challenge
acceptableCAUVC:Q9A223Navick, Ca$h Money Curators2019-01-29 15:57:04 CSTGO:0000160 phosphorelay signal transduction system (P)PMID:16176121ECO:0001202 in vitro protein kinase assay evidence used in manual assertion

Figure 5 E shows Caulobacter crescentus protein cenR (CC3743/Cell Envelope Regulator) is involved in the phosphorelay signal transduction system.

challenge

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