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BPP4:PRIM
Contents
Species (Taxon ID) | Enterobacteria phage P4 (Bacteriophage P4). (10680) | |
Gene Name(s) | Alpha | |
Protein Name(s) | Putative P4-specific DNA primase | |
External Links | ||
UniProt | P10277 | |
EMBL | X05623 X51522 | |
RefSeq | NP_042036.1 | |
PDB | 1KA8 | |
PDBsum | 1KA8 | |
ProteinModelPortal | P10277 | |
SMR | P10277 | |
GeneID | 1261095 | |
KEGG | vg:1261095 | |
KO | K06919 | |
EvolutionaryTrace | P10277 | |
Proteomes | UP000009093 | |
GO | GO:0005524 GO:0003677 GO:0003896 GO:0004386 GO:0008270 | |
Gene3D | 1.10.10.10 3.40.50.300 3.90.580.10 | |
InterPro | IPR004968 IPR014015 IPR027417 IPR014818 IPR013237 IPR006171 IPR011991 IPR002694 | |
Pfam | PF08706 PF03288 PF08273 PF13362 | |
SMART | SM00885 SM00778 SM00493 | |
SUPFAM | SSF46785 SSF52540 | |
PROSITE | PS51206 PS50880 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0003896 |
DNA primase activity |
ECO:0000314 |
F |
The P4 alpha protein was placed under the control of the LacI operon in two different recombinant plasmids. A direct assay intended to measure the ability of a primase to initiate synthesis of DNA by measuring the amount of thymine incorporated into the strand was conducted using cells induced to produce the alpha protein via the LacI operon, non-induced cells, and purified alpha protein. The induced cells and purified alpha protein showed high amounts of incorporated thymine, indicating primase activity. The non-induced cells showed no thymine incorporation (Fig 8, p. 13068), demonstrating that the alpha protein has primase activity. |
complete | |||||
enables |
GO:0003896 |
DNA primase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0004386 |
helicase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0008270 |
zinc ion binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0004386 |
helicase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003899 |
DNA-directed 5'-3' RNA polymerase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0006260 |
DNA replication |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0006269 |
DNA replication, synthesis of RNA primer |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Strack, B et al. (1992) A common sequence motif, -E-G-Y-A-T-A-, identified within the primase domains of plasmid-encoded I- and P-type DNA primases and the alpha protein of the Escherichia coli satellite phage P4. J. Biol. Chem. 267 13062-72 PubMed GONUTS page