GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

BPP4:PRIM

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Enterobacteria phage P4 (Bacteriophage P4). (10680)
Gene Name(s) Alpha
Protein Name(s) Putative P4-specific DNA primase
External Links
UniProt P10277
EMBL X05623
X51522
RefSeq NP_042036.1
PDB 1KA8
PDBsum 1KA8
ProteinModelPortal P10277
SMR P10277
GeneID 1261095
KEGG vg:1261095
KO K06919
EvolutionaryTrace P10277
Proteomes UP000009093
GO GO:0005524
GO:0003677
GO:0003896
GO:0004386
GO:0008270
Gene3D 1.10.10.10
3.40.50.300
3.90.580.10
InterPro IPR004968
IPR014015
IPR027417
IPR014818
IPR013237
IPR006171
IPR011991
IPR002694
Pfam PF08706
PF03288
PF08273
PF13362
SMART SM00885
SM00778
SM00493
SUPFAM SSF46785
SSF52540
PROSITE PS51206
PS50880

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0003896

DNA primase activity

PMID:1618804[1]

ECO:0000314

F

The P4 alpha protein was placed under the control of the LacI operon in two different recombinant plasmids. A direct assay intended to measure the ability of a primase to initiate synthesis of DNA by measuring the amount of thymine incorporated into the strand was conducted using cells induced to produce the alpha protein via the LacI operon, non-induced cells, and purified alpha protein. The induced cells and purified alpha protein showed high amounts of incorporated thymine, indicating primase activity. The non-induced cells showed no thymine incorporation (Fig 8, p. 13068), demonstrating that the alpha protein has primase activity.

complete
CACAO 11889

enables

GO:0003896

DNA primase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013237

F

Seeded From UniProt

complete

enables

GO:0004386

helicase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013237

F

Seeded From UniProt

complete

enables

GO:0008270

zinc ion binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR013237

F

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

enables

GO:0046872

metal ion binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0479

F

Seeded From UniProt

complete

enables

GO:0004386

helicase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0347

F

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0067

F

Seeded From UniProt

complete

enables

GO:0016779

nucleotidyltransferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0548

F

Seeded From UniProt

complete

enables

GO:0003899

DNA-directed 5'-3' RNA polymerase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0240

F

Seeded From UniProt

complete

involved_in

GO:0006260

DNA replication

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0235

P

Seeded From UniProt

complete

involved_in

GO:0006269

DNA replication, synthesis of RNA primer

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0639

P

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Strack, B et al. (1992) A common sequence motif, -E-G-Y-A-T-A-, identified within the primase domains of plasmid-encoded I- and P-type DNA primases and the alpha protein of the Escherichia coli satellite phage P4. J. Biol. Chem. 267 13062-72 PubMed GONUTS page