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2024-03-29T11:40:33Z
User contributions
MediaWiki 1.31.1
https://gowiki.tamu.edu/wiki/index.php?title=BPT7:DPOL&diff=5909835
BPT7:DPOL
2018-04-23T00:47:21Z
<p>AHora: Table edited by $2 via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Enterobacteria phage T7 (Bacteriophage T7).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10760 10760]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || No Information Provided. <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || DNA-directed DNA polymerase<br />
DNA polymerase gp5<br />
Gene product 5<br />
Gp5<br />
T7 DNA polymerase<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P00581 P00581]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=A00716 A00716]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041982.1 NP_041982.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SKR 1SKR]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SKS 1SKS]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SKW 1SKW]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SL0 1SL0]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SL1 1SL1]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1SL2 1SL2]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1T7P 1T7P]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1T8E 1T8E]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TK0 1TK0]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TK5 1TK5]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TK8 1TK8]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TKD 1TKD]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1X9M 1X9M]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1X9S 1X9S]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1X9W 1X9W]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1ZYQ 1ZYQ]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2AJQ 2AJQ]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDBsum<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1SKR<br />1SKS<br />1SKW<br />1SL0<br />1SL1<br />1SL2<br />1T7P<br />1T8E<br />1TK0<br />1TK5<br />1TK8<br />1TKD<br />1X9M<br />1X9S<br />1X9W<br />1ZYQ<br />2AJQ<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | ProteinModelPortal<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P00581<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P00581<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | DIP<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>DIP-41665N<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | IntAct<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/intact/search/do/search?searchString=P00581 P00581]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | MINT<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>MINT-1513442<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261044<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | EvolutionaryTrace<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P00581<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000000840<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0003677 GO:0003677]<br />[http://amigo.geneontology.org/amigo/term/GO:0003887 GO:0003887]<br />[http://amigo.geneontology.org/amigo/term/GO:0004527 GO:0004527]<br />[http://amigo.geneontology.org/amigo/term/GO:0006261 GO:0006261]<br />[http://amigo.geneontology.org/amigo/term/GO:0016032 GO:0016032]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Gene3D<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://gene3d.biochem.ucl.ac.uk/superfamily/?accession=3.30.420.10 3.30.420.10]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR019760 IPR019760]<br />[http://www.ebi.ac.uk/interpro/entry/IPR001098 IPR001098]<br />[http://www.ebi.ac.uk/interpro/entry/IPR002298 IPR002298]<br />[http://www.ebi.ac.uk/interpro/entry/IPR012337 IPR012337]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF00476 PF00476]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PRINTS<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.bioinf.manchester.ac.uk/cgi-bin/dbbrowser/sprint/searchprintss.cgi?display_opts=Prints&category=None&queryform=false&regexpr=off&prints_accn=PR00868 PR00868]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMART<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00482 SM00482]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SUPFAM<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>SSF53098<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PROSITE<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>PS00447<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.770147.F4e6ff15d180ac--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F4e6ff15d180ac" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0003887<br />
|<br />
DNA-directed DNA polymerase activity<br />
|<br />
PMID:23012374<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
F<br />
|<br />
Fig 3<br />
|<br />
complete<br/>[[Special:CACAO/annotation/11449|CACAO 11449]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0003676<br />
|<br />
nucleic acid binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR012337<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0003677<br />
|<br />
DNA binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR001098<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0003677<br />
|<br />
DNA binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0238<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0003887<br />
|<br />
DNA-directed DNA polymerase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR001098<br />InterPro:IPR002298<br />InterPro:IPR019760<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0003887<br />
|<br />
DNA-directed DNA polymerase activity<br />
|<br />
GO_REF:0000003<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
EC:2.7.7.7<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0003887<br />
|<br />
DNA-directed DNA polymerase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0239<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0004518<br />
|<br />
nuclease activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0540<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0004527<br />
|<br />
exonuclease activity<br />
|<br />
2703498:2703498<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
F<br />
|<br />
The experiment shows that mutation at His residue within the gene cause inactive exonuclease.<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0090592<br />
|<br />
DNA synthesis involved in DNA replication<br />
|<br />
PMID:21606333<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
P<br />
|<br />
Figure 2 compared the activities of both gp5/trx and its mutated form Gp5-Fbpneu/trx in the presence of gp4 by looking at how much nucleotide (dTMP) had been incorporated in a sample of dsDNA . According to the paper and from the results, Gp5-Fbpneu/trx did not mediate the synthesis in the presence of gp4 over a large range of concentrations of gp5/trx and gp4. This means that a mutated gp5 would result in disrupted DNA synthesis in the presence of gp4 as compared to a normal version of gp5, showing its critical function for DNA replication.<br />
|<br />
complete<br/>[[Special:CACAO/annotation/13104|CACAO 13104]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0004527<br />
|<br />
exonuclease activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0269<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0004529<br />
|<br />
exodeoxyribonuclease activity<br />
|<br />
PMID:1846298<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
F<br />
|<br />
Table 3 (specifically k_exo) compares the rates of exonuclease activity between the wild-type enzyme (105 1/s) and mutant enzyme (6.9e-4 1/s), revealing that the wild-type exonuclease rates are dramatically higher than that of the mutant enzyme's exonuclease rates. This shows that this domain of DNA polymerase has exonuclease activity.<br />
|<br />
complete<br/>[[Special:CACAO/annotation/11980|CACAO 11980]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR001098<br />InterPro:IPR019760<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0235<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0006261<br />
|<br />
DNA-dependent DNA replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR002298<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0016032<br />
|<br />
viral process<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0945<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0016740<br />
|<br />
transferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0808<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0016779<br />
|<br />
nucleotidyltransferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0548<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0039693<br />
|<br />
viral DNA genome replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-1194<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0071897<br />
|<br />
DNA biosynthetic process<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR001098<br />InterPro:IPR002298<br />InterPro:IPR019760<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0071897<br />
|<br />
DNA biosynthetic process<br />
|<br />
GO_REF:0000003<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
EC:2.7.7.7<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0071897<br />
|<br />
DNA biosynthetic process<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0239<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0090305<br />
|<br />
nucleic acid phosphodiester bond hydrolysis<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0269<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
|- <br />
|<br />
<br />
|<br />
GO:0090305<br />
|<br />
nucleic acid phosphodiester bond hydrolysis<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0540<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.770147.F4e6ff15d180ac&page=770147&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
|}<br />
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.770147.F4e6ff15d180ac--><br />
</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Autographivirinae]]<br />
[[Category: T7likevirus]]<br />
[[Category: Enterobacteria phage T7]]<br />
[[Category: Enterobacteria phage T7]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPCP1:VLYS&diff=5909719
BPCP1:VLYS
2018-04-16T03:03:05Z
<p>AHora: Table edited by $2 via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Streptococcus phage Cp-1 (Bacteriophage Cp-1).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10747 10747]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || CPH1 (synonyms: <i> 21</i>)<br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || Holin<br />
Lysis protein<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/Q38008 Q38008]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=Z47794 Z47794]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_044836.1 NP_044836.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | TCDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1.E.16.1.1<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261217<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1261217]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000009089<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0033644 GO:0033644]<br />[http://amigo.geneontology.org/amigo/term/GO:0016021 GO:0016021]<br />[http://amigo.geneontology.org/amigo/term/GO:0019835 GO:0019835]<br />[http://amigo.geneontology.org/amigo/term/GO:0019076 GO:0019076]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR006480 IPR006480]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF05105 PF05105]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | TIGRFAMs<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>TIGR01593<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921892.U571e471730a93--><br />
<!--<br />
******************************************************************************************<br />
* <br />
** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br />
* <br />
****************************************************************************************** --><br />
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U571e471730a93" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0016020<br />
|<br />
membrane<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0472<br />
|<br />
C<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016021<br />
|<br />
integral component of membrane<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0812<br />
|<br />
C<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0019076<br />
|<br />
viral release from host cell<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-1188<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0019835<br />
|<br />
cytolysis<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0204<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0033644<br />
|<br />
host cell membrane<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-1043<br />
|<br />
C<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0033644<br />
|<br />
host cell membrane<br />
|<br />
GO_REF:0000039<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-SubCell:SL-0380<br />
|<br />
C<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0019835<br />
|<br />
cytolysis<br />
|<br />
PMID:9440507<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
P<br />
|<br />
Refer to Figure 3. Various cultures of E. coli were grown with both induced and uninduced cph1 and cp11 genes. The E.coli cultures that showed induced forms of the genes (cph1 produces holin) had lower recorded densities and viabilities compared to cultures that had the uninduced genes. The paper notes that cp11 alone does not affect the viability or the density of the cultures, which further supports the claim that cph1 is needed to produce holins that can weaken the membrane of the host cell and cause it to undergo lysis.<br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.921892.U571e471730a93&page=921892&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
|}<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921892.U571e471730a93--><br />
</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Picovirinae]]<br />
[[Category: unassigned Picovirinae]]<br />
[[Category: Streptococcus phage Cp-1]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPT7:PRIM&diff=5909374
BPT7:PRIM
2018-04-09T02:35:16Z
<p>AHora: Table edited by $2 via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Enterobacteria phage T7 (Bacteriophage T7).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10760 10760]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || No Information Provided. <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || DNA primase/helicase<br />
Gene product 4<br />
Gp4<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P03692 P03692]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01127 V01127]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=A04314 A04314]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041975.1 NP_041975.1]<br />[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041977.1 NP_041977.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR0 1CR0]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR1 1CR1]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR2 1CR2]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR4 1CR4]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0J 1E0J]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0K 1E0K]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NUI 1NUI]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1Q57 1Q57]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDBsum<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1CR0<br />1CR1<br />1CR2<br />1CR4<br />1E0J<br />1E0K<br />1NUI<br />1Q57<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | ProteinModelPortal<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | IntAct<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/intact/search/do/search?searchString=P03692 P03692]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | MINT<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>MINT-1513747<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261046<br />1261048<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1261046]<br />[http://www.genome.ad.jp/kegg/ vg:1261048]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>K17680<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | EvolutionaryTrace<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000000840<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0043139 GO:0043139]<br />[http://amigo.geneontology.org/amigo/term/GO:0005524 GO:0005524]<br />[http://amigo.geneontology.org/amigo/term/GO:0003896 GO:0003896]<br />[http://amigo.geneontology.org/amigo/term/GO:0003697 GO:0003697]<br />[http://amigo.geneontology.org/amigo/term/GO:0008270 GO:0008270]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Gene3D<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://gene3d.biochem.ucl.ac.uk/superfamily/?accession=3.40.50.300 3.40.50.300]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR007694 IPR007694]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027417 IPR027417]<br />[http://www.ebi.ac.uk/interpro/entry/IPR013237 IPR013237]<br />[http://www.ebi.ac.uk/interpro/entry/IPR006171 IPR006171]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027032 IPR027032]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PANTHER<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.pantherdb.org/panther/lookupId.jsp?id=PTHR12873 PTHR12873]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF03796 PF03796]<br />[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF08273 PF08273]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMART<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00778 SM00778]<br />[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00493 SM00493]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SUPFAM<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>SSF52540<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PROSITE<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>PS51199<br />PS50880<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3--><br />
<!--<br />
******************************************************************************************<br />
* <br />
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br />
* <br />
****************************************************************************************** --><br />
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I570be2394c9b3" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0000166<br />
|<br />
nucleotide binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0547<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003678<br />
|<br />
DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003697<br />
|<br />
single-stranded DNA binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003899<br />
|<br />
DNA-directed RNA polymerase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0240<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0347<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0067<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0235<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006269<br />
|<br />
DNA replication, synthesis of RNA primer<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0639<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0008270<br />
|<br />
zinc ion binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016740<br />
|<br />
transferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0808<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016779<br />
|<br />
nucleotidyltransferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0548<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0043139<br />
|<br />
5'-3' DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0046872<br />
|<br />
metal ion binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0479<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
PMID:11279245<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
F<br />
|<br />
Figs. 5, 6. "An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase."<br />
|<br />
complete<br/>[[Special:CACAO/annotation/11625|CACAO 11625]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0006269<br />
|<br />
DNA replication, synthesis of RNA primer<br />
|<br />
PMID:21606333<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
P<br />
|<br />
"The primase domain of gp4 catalyzes the synthesis of RNA primer for gp5/trx to initiate DNA synthesis on ssDNA template. gp5-Fbpneu/trx mediates RNA-dependent DNA synthesis on M13 ssDNA with gp4 approximately one-third to one-half as well as does wild-type gp5/trx (Fig. S2)". The figure shows that the primase activity of gp4 is important in DNA synthesis in conjunction with both gp5/trx and its mutated counterpart.<br />
|<br />
complete<br/>[[Special:CACAO/annotation/13104|CACAO 13104]] <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3&page=921272&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
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</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Autographivirinae]]<br />
[[Category: T7likevirus]]<br />
[[Category: Enterobacteria phage T7]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPP2:SCAF&diff=5909119
BPP2:SCAF
2018-03-30T02:16:43Z
<p>AHora: Table edited by AHora via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Escherichia phage P2 (Bacteriophage P2).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10679 10679]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || O <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || Capsid assembly scaffolding protein ([http://microbialphenotypes.org/wiki/index.php/ECO:0000305 ECO:0000305])<br />
Gene product O<br />
GpO<br />
Head morphogenesis protein ([http://microbialphenotypes.org/wiki/index.php/ECO:0000305 ECO:0000305])<br />
Scaffold protein ([http://microbialphenotypes.org/wiki/index.php/ECO:0000305 ECO:0000305])<br />
Maturation protease ([http://microbialphenotypes.org/wiki/index.php/ECO:0000305 ECO:0000305] with [[PMID:19064277]]<ref name='PMID:19064277'/>)<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P25478 P25478]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=AF063097 AF063097]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=S22798 S22798]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_046759.1 NP_046759.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | MEROPS<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://merops.sanger.ac.uk/cgi-bin/pepsum?mid=S73.001 S73.001]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261524<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1261524]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | OrthoDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>VOG090000ND<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000009092<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0008236 GO:0008236]<br />[http://amigo.geneontology.org/amigo/term/GO:0046797 GO:0046797]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR009228 IPR009228]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pfam.xfam.org/family/PF05929 PF05929]<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.929049.E5aba7e15d30d4--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="E5aba7e15d30d4" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0006508<br />
|<br />
proteolysis<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0645<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0008233<br />
|<br />
peptidase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0645<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0008236<br />
|<br />
serine-type peptidase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0720<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0019069<br />
|<br />
viral capsid assembly<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR009228<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0046797<br />
|<br />
viral procapsid maturation<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-1273<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006508<br />
|<br />
proteolysis<br />
|<br />
PMID:16457867<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
P<br />
|<br />
Figure 2. A fusion of gpO and MBP was created by the researchers to see the function of gpO since its product is unstable on its own. An SDS-PAGE was done on both the normal fusion (MBP:gpO) and a mutation where gpO lacked the first N-terminal 25 amino acids (MBP:OΔ25). The normal fusion accumulated a high concentration of proteins at about 40 minutes before disappearing, while the mutant with the deletion accumulated proteins more than 2 hours into the experiment. The disappearance of the protein fusion showed the proteolytic activity of gpO. The results also indicated that gpO has auto-proteolytic activity and that this is disrupted with the deletion of the N-terminal 25 amino acids.<br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.929049.E5aba7e15d30d4&page=929049&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
|}<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.929049.E5aba7e15d30d4--><br />
</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Myoviridae]]<br />
[[Category: Peduovirinae]]<br />
[[Category: P2virus]]<br />
[[Category: Escherichia phage P2]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:29255010&diff=5908762
PMID:29255010
2018-03-20T21:20:50Z
<p>AHora: Fill PMID: Page!</p>
<hr />
<div>{{RightTOC}}<br />
<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.929011.H5ab17b2eb033f--><br />
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* <br />
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br />
* <br />
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{| id="H5ab17b2eb033f" class=" tableEdit PMID_info_table" <br />
<br />
|-<br />
!align=left |Citation<br />
||<br />
'''Nazarov, S, Schneider, JP, Brackmann, M, Goldie, KN, Stahlberg, H and Basler, M''' (2018) Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end. ''EMBO J.'' '''37'''<br />
|-<br />
!align=left |Abstract<br />
||<br />
The bacterial Type VI secretion system (T6SS) assembles from three major parts: a membrane complex that spans inner and outer membranes, a baseplate, and a sheath-tube polymer. The baseplate assembles around a tip complex with associated effectors and connects to the membrane complex by TssK. The baseplate assembly initiates sheath-tube polymerization, which in some organisms requires TssA. Here, we analyzed both ends of isolated non-contractilesheaths by cryo-electron microscopy. Our analysis suggests that the baseplate, solved to an average 8.0Â Ã… resolution, is composed of six subunits of TssE/F/G and the baseplate periphery is decorated by six TssK trimers. The VgrG/PAAR tip complex in the center of the baseplate is surrounded by a cavity, which may accommodate up to ~450Â kDa of effector proteins. The distal end of the sheath, resolved to an average 7.5Â Ã… resolution, shows sixfold symmetry; however, its protein composition is unclear. Our structures provide an important step toward an atomic model of the complete T6SS assembly.<br />
|-<br />
!align=left |Links<br />
||<br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=29255010 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813253 PMC5813253]<br />
Online version:[http://dx.doi.org/10.15252/embj.201797103 10.15252/embj.201797103]<br />
|-<br />
!align=left |Keywords<br />
||<br />
<br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.929011.H5ab17b2eb033f&page=929011&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||<br />
|}<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.929011.H5ab17b2eb033f--><br />
<br />
== Significance ==<br />
<br />
== Annotations ==<br />
<annotationlinks/><br />
<br />
== Notes ==<br />
<br />
== See also ==<br />
<br />
== References ==<br />
{{RefHelp}}<br />
<references/></div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:29255010&diff=5908761
PMID:29255010
2018-03-20T21:20:46Z
<p>AHora: New PMID: Page!</p>
<hr />
<div>PMID on Demand placeholder</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPPH2:GP17&diff=5908714
BPPH2:GP17
2018-03-19T02:17:26Z
<p>AHora: Table edited by AHora via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Bacillus phage phi29 (Bacteriophage phi-29).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10756 10756]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || 17 <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || DNA replication protein 17<br />
Gene product 17<br />
gp17<br />
Protein p17<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P03686 P03686]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=M14430 M14430]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=EU771092 EU771092]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=JN0030 JN0030]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=YP_002004550.1 YP_002004550.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | TCDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>9.B.80.1.1<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>6446521<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:6446521]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | OrthoDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>VOG0900019F<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000001207<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0006260 GO:0006260]<br />[http://amigo.geneontology.org/amigo/term/GO:0039693 GO:0039693]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR035184 IPR035184]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pfam.xfam.org/family/PF17549 PF17549]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | ProDom<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://prodom.prabi.fr/prodom/current/cgi-bin/request.pl?question=DBEN&query=PD021825 PD021825]<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.929000.R5aaf1a08a2fe4--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R5aaf1a08a2fe4" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0235<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0039693<br />
|<br />
viral DNA genome replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR035184<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0039693<br />
|<br />
viral DNA genome replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-1194<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0039693<br />
|<br />
viral DNA genome replication<br />
|<br />
PMID:9858710<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
-<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0039693<br />
|<br />
viral DNA genome replication<br />
|<br />
PMID:9858710<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
P<br />
|<br />
Figure 6. An "amplification assay was carried out in the presence of 5 ng of φ29 DNA, purified φ29 replication proteins (TP, DNA polymerase, DBP and SSB), either in the absence (control) or in the presence of protein p17". The results indicated that DNA amplification significantly increases in the presence of p17 proteins compared to when it was absent, showing that the protein has a role in viral DNA replication.<br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
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|}<br />
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</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Picovirinae]]<br />
[[Category: Phi29virus]]<br />
[[Category: Bacillus phage phi29]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:18281701&diff=5908575
PMID:18281701
2018-03-16T16:45:41Z
<p>AHora: Fill PMID: Page!</p>
<hr />
<div>{{RightTOC}}<br />
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{| id="L5aabf4b0209ed" class=" tableEdit PMID_info_table" <br />
<br />
|-<br />
!align=left |Citation<br />
||<br />
'''Brok-Volchanskaya, VS, Kadyrov, FA, Sivogrivov, DE, Kolosov, PM, Sokolov, AS, Shlyapnikov, MG, Kryukov, VM and Granovsky, IE''' (2008) Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage. ''Nucleic Acids Res.'' '''36''':2094-105<br />
|-<br />
!align=left |Abstract<br />
||<br />
Homing endonucleases initiate nonreciprocal transfer of DNA segments containing their own genes and the flanking sequences by cleaving the recipient DNA. Bacteriophage T4 segB gene, which is located in a cluster of tRNA genes, encodes a protein of unknown function, homologous to homing endonucleases of the GIY-YIG family. We demonstrate that SegB protein is a site-specific endonuclease, which produces mostly 3' 2-nt protruding ends at its DNA cleavage site. Analysis of SegB cleavage sites suggests that SegB recognizes a 27-bp sequence. It contains 11-bp conserved sequence, which corresponds to a conserved motif of tRNA TpsiC stem-loop, whereas the remainder of the recognition site is rather degenerate. T4-related phages T2L, RB1 and RB3 contain tRNA gene regions that are homologous to that of phage T4 but lack segB gene and several tRNA genes. In co-infections of phages T4 and T2L, segB gene is inherited with nearly 100% of efficiency. The preferred inheritance depends absolutely on the segB gene integrity and is accompanied by the loss of the T2L tRNA gene region markers. We suggest that SegB is a homing endonuclease that functions to ensure spreading of its own gene and the surrounding tRNA genes among T4-related phages.<br />
|-<br />
!align=left |Links<br />
||<br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18281701 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330249 PMC2330249]<br />
Online version:[http://dx.doi.org/10.1093/nar/gkn053 10.1093/nar/gkn053]<br />
|-<br />
!align=left |Keywords<br />
||<br />
Bacteriophage T4/enzymology; Bacteriophage T4/genetics; Base Sequence; Conserved Sequence; DNA, Viral/chemistry; DNA, Viral/metabolism; Endodeoxyribonucleases/genetics; Endodeoxyribonucleases/metabolism; Gene Conversion; Inheritance Patterns; Myoviridae/genetics; Open Reading Frames; RNA, Transfer/genetics; Substrate Specificity; T-Phages/genetics; Viral Proteins/genetics; Viral Proteins/metabolism<br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.928964.L5aabf4b0209ed&page=928964&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||<br />
|}<br />
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<br />
== Significance ==<br />
<br />
== Annotations ==<br />
<annotationlinks/><br />
<br />
== Notes ==<br />
<br />
== See also ==<br />
<br />
== References ==<br />
{{RefHelp}}<br />
<references/></div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:18281701&diff=5908574
PMID:18281701
2018-03-16T16:45:36Z
<p>AHora: New PMID: Page!</p>
<hr />
<div>PMID on Demand placeholder</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPT4:SEGB&diff=5908573
BPT4:SEGB
2018-03-16T16:43:55Z
<p>AHora: Table edited by AHora via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Enterobacteria phage T4 (Bacteriophage T4).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10665 10665]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || segB (synonyms: <i> trnA.1</i>)<br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || Putative endonuclease segB<br />
Endodeoxyribonuclease segB<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P13325 P13325]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=X03016 X03016]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=Z69338 Z69338]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=AF158101 AF158101]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_049745.2 NP_049745.2]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1258659<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1258659]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | OrthoDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>VOG090000QM<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000009087<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0004519 GO:0004519]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Gene3D<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://gene3d.biochem.ucl.ac.uk/search?mode=family&sterm=3.40.1440.10 3.40.1440.10]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR000305 IPR000305]<br />[http://www.ebi.ac.uk/interpro/entry/IPR035901 IPR035901]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pfam.xfam.org/family/PF01541 PF01541]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMART<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00465 SM00465]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SUPFAM<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>SSF82771<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PROSITE<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>PS50164<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.928963.K5aabe0d9a96f6--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K5aabe0d9a96f6" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0004518<br />
|<br />
nuclease activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0540<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004519<br />
|<br />
endonuclease activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0255<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0090305<br />
|<br />
nucleic acid phosphodiester bond hydrolysis<br />
|<br />
GO_REF:0000108<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
GO:0004518<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0090305<br />
|<br />
nucleic acid phosphodiester bond hydrolysis<br />
|<br />
GO_REF:0000108<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
GO:0004519<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004519<br />
|<br />
endonuclease activity<br />
|<br />
PMID:18281701<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
F<br />
|<br />
Figure 4. SegB endonuclease cleaves DNA at sites that have different susceptibility for the enzyme cleavage. Figure 4 shows enzyme products of SepB at different concentrations with different phage samples. These results indicate that SepB has relaxed specificity to DNA fragments.<br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.928963.K5aabe0d9a96f6&page=928963&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
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</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Myoviridae]]<br />
[[Category: Tevenvirinae]]<br />
[[Category: T4virus]]<br />
[[Category: Enterobacteria phage T4]]</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:3863101&diff=5908470
PMID:3863101
2018-03-14T15:38:07Z
<p>AHora: Fill PMID: Page!</p>
<hr />
<div>{{RightTOC}}<br />
<br />
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{| id="R5aa941da33850" class=" tableEdit PMID_info_table" <br />
<br />
|-<br />
!align=left |Citation<br />
||<br />
'''Blanco, L and Salas, M''' (1985) Replication of phage phi 29 DNA with purified terminal protein and DNA polymerase: synthesis of full-length phi 29 DNA. ''Proc. Natl. Acad. Sci. U.S.A.'' '''82''':6404-8<br />
|-<br />
!align=left |Abstract<br />
||<br />
A system that replicates bacteriophage phi 29 DNA with protein p3 covalently attached to the two 5' ends, using as the only proteins the phi 29 DNA polymerase and the terminal protein, is described. Restriction analysis of the 32P-labeled DNA synthesized in vitro showed that all phi 29 DNA fragments were labeled. Analysis by alkaline sucrose gradient centrifugation of the DNA labeled during a 10-min pulse showed that, after a 20-min chase, about half of the DNA molecules had reached apparently full-length phi 29 DNA (approximately equal to 18,000 nucleotides). Ammonium ions strongly stimulated phi 29 DNA-protein p3 replication, the effect being due to stimulation of the initiation reaction. ATP was not required for phi 29 DNA-protein p3 replication, either in the initiation or elongation steps. The results show that the phi 29 DNA polymerase functions, not only in the formation of the p3-dAMP covalent initiation complex but also in the elongation of the latter, as the only DNA polymerase to produce full-length phi 29 DNA.<br />
|-<br />
!align=left |Links<br />
||<br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3863101 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC390724 PMC390724]<br />
<br />
|-<br />
!align=left |Keywords<br />
||<br />
Adenosine Monophosphate/metabolism; Adenosine Triphosphate/metabolism; Bacteriophages/enzymology; Bacteriophages/genetics; DNA Replication; DNA, Viral/biosynthesis; DNA-Directed DNA Polymerase/metabolism; Viral Proteins/metabolism; Viral Proteins/physiology; Virus Replication<br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.928932.R5aa941da33850&page=928932&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||<br />
|}<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.928932.R5aa941da33850--><br />
<br />
== Significance ==<br />
<br />
== Annotations ==<br />
<annotationlinks/><br />
<br />
== Notes ==<br />
<br />
== See also ==<br />
<br />
== References ==<br />
{{RefHelp}}<br />
<references/></div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=PMID:3863101&diff=5908469
PMID:3863101
2018-03-14T15:38:02Z
<p>AHora: New PMID: Page!</p>
<hr />
<div>PMID on Demand placeholder</div>
AHora
https://gowiki.tamu.edu/wiki/index.php?title=BPT7:PRIM&diff=5908112
BPT7:PRIM
2018-03-12T04:54:25Z
<p>AHora: Table edited by AHora via TableEdit</p>
<hr />
<div><br />
<div style="float:right; margin: 1em;"> __TOC__</div><br />
<div id="infobox"><br />
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Enterobacteria phage T7 (Bacteriophage T7).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10760 10760]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || No Information Provided. <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || DNA primase/helicase<br />
Gene product 4<br />
Gp4<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P03692 P03692]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01127 V01127]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=A04314 A04314]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041975.1 NP_041975.1]<br />[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041977.1 NP_041977.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR0 1CR0]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR1 1CR1]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR2 1CR2]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR4 1CR4]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0J 1E0J]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0K 1E0K]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NUI 1NUI]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1Q57 1Q57]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDBsum<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1CR0<br />1CR1<br />1CR2<br />1CR4<br />1E0J<br />1E0K<br />1NUI<br />1Q57<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | ProteinModelPortal<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | IntAct<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/intact/search/do/search?searchString=P03692 P03692]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | MINT<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>MINT-1513747<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261046<br />1261048<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1261046]<br />[http://www.genome.ad.jp/kegg/ vg:1261048]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>K17680<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | EvolutionaryTrace<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000000840<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0043139 GO:0043139]<br />[http://amigo.geneontology.org/amigo/term/GO:0005524 GO:0005524]<br />[http://amigo.geneontology.org/amigo/term/GO:0003896 GO:0003896]<br />[http://amigo.geneontology.org/amigo/term/GO:0003697 GO:0003697]<br />[http://amigo.geneontology.org/amigo/term/GO:0008270 GO:0008270]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Gene3D<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://gene3d.biochem.ucl.ac.uk/superfamily/?accession=3.40.50.300 3.40.50.300]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR007694 IPR007694]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027417 IPR027417]<br />[http://www.ebi.ac.uk/interpro/entry/IPR013237 IPR013237]<br />[http://www.ebi.ac.uk/interpro/entry/IPR006171 IPR006171]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027032 IPR027032]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PANTHER<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.pantherdb.org/panther/lookupId.jsp?id=PTHR12873 PTHR12873]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF03796 PF03796]<br />[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF08273 PF08273]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMART<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00778 SM00778]<br />[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00493 SM00493]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SUPFAM<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>SSF52540<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PROSITE<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>PS51199<br />PS50880<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I570be2394c9b3" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0000166<br />
|<br />
nucleotide binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0547<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003678<br />
|<br />
DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003697<br />
|<br />
single-stranded DNA binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003899<br />
|<br />
DNA-directed RNA polymerase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0240<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0347<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0067<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0235<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006269<br />
|<br />
DNA replication, synthesis of RNA primer<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0639<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0008270<br />
|<br />
zinc ion binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016740<br />
|<br />
transferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0808<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016779<br />
|<br />
nucleotidyltransferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0548<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0043139<br />
|<br />
5'-3' DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0046872<br />
|<br />
metal ion binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0479<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
PMID:11279245<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
F<br />
|<br />
Figs. 5, 6. "An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase."<br />
|<br />
complete<br/>[[Special:CACAO/annotation/11625|CACAO 11625]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA helicase activity<br />
|<br />
PMID:21606333<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
F<br />
|<br />
The experiment measures DNA replication and the impediments faced when missing gene 5. Bacteriophage T7 cannot grow in cell that have gp5, because neutral residues, Asparagine, were used instead of the basic residues. The major effects seen on T7 were a decrease in polymerase activity on the ssDNA template and reduced processivity. Perhaps, this is because of the mutations in gene 5, which changed the binding site for one or more of the other T7 replication proteins. When the C-terminal tail is removed, the ability of gp4 to mediate the strand-displacement synthesis is obliterated. Please refer to Figure 2. <br />
|<br />
complete<br/>[[Special:CACAO/annotation/13104|CACAO 13104]] <br />
<br />
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<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Autographivirinae]]<br />
[[Category: T7likevirus]]<br />
[[Category: Enterobacteria phage T7]]</div>
AHora
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<p>AHora: Fill PMID: Page!</p>
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{| id="D5a9cc7b7d4bf9" class=" tableEdit PMID_info_table" <br />
<br />
|-<br />
!align=left |Citation<br />
||<br />
'''Zhang, H, Lee, SJ, Zhu, B, Tran, NQ, Tabor, S and Richardson, CC''' (2011) Helicase-DNA polymerase interaction is critical to initiate leading-strand DNA synthesis. ''Proc. Natl. Acad. Sci. U.S.A.'' '''108''':9372-7<br />
|-<br />
!align=left |Abstract<br />
||<br />
Interactions between gene 4 helicase and gene 5 DNA polymerase (gp5) are crucial for leading-strand DNA synthesis mediated by the replisome of bacteriophage T7. Interactions between the two proteins that assure high processivity are known but the interactions essential to initiate the leading-strand DNA synthesis remain unidentified. Replacement of solution-exposed basic residues (K587, K589, R590, and R591) located on the front surface of gp5 with neutral asparagines abolishes the ability of gp5 and the helicase to mediate strand-displacement synthesis. This front basic patch in gp5 contributes to physical interactions with the acidic C-terminal tail of the helicase. Nonetheless, the altered polymerase is able to replace gp5 and continue ongoing strand-displacement synthesis. The results suggest that the interaction between the C-terminal tail of the helicase and the basic patch of gp5 is critical for initiation of strand-displacement synthesis. Multiple interactions of T7 DNA polymerase and helicase coordinate replisome movement.<br />
|-<br />
!align=left |Links<br />
||<br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21606333 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111293 PMC3111293]<br />
Online version:[http://dx.doi.org/10.1073/pnas.1106678108 10.1073/pnas.1106678108]<br />
|-<br />
!align=left |Keywords<br />
||<br />
Bacteriophage T7/genetics; Bacteriophage T7/metabolism; Binding Sites/genetics; DNA Helicases/chemistry; DNA Helicases/genetics; DNA Helicases/metabolism; DNA Replication; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; DNA, Viral/chemistry; DNA, Viral/genetics; DNA, Viral/metabolism; DNA-Directed DNA Polymerase/chemistry; DNA-Directed DNA Polymerase/genetics; DNA-Directed DNA Polymerase/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Models, Molecular; Mutation; Nucleic Acid Conformation; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Surface Plasmon Resonance; Thioredoxins/chemistry; Thioredoxins/genetics; Thioredoxins/metabolism; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/metabolism<br />
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<br />
== Significance ==<br />
<br />
== Annotations ==<br />
<annotationlinks/><br />
<br />
== Notes ==<br />
<br />
== See also ==<br />
<br />
== References ==<br />
{{RefHelp}}<br />
<references/></div>
AHora
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PMID:21606333
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AHora
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2018-03-05T04:16:08Z
<p>AHora: Table edited by AHora via TableEdit</p>
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{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" width= '70%'<br />
|-<br />
|bgcolor='#CCCCFF'| '''Species (Taxon ID)''' || <i>Enterobacteria phage T7 (Bacteriophage T7).</i> ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10760 10760]) <br />
|-<br />
|bgcolor='#CCCCFF' |'''Gene Name(s)''' || No Information Provided. <br />
|-<br />
|bgcolor='#CCCCFF'| '''Protein Name(s)''' || DNA primase/helicase<br />
Gene product 4<br />
Gp4<br />
|-<br />
| align="center" colspan="3" bgcolor="#dddddd" | '''External Links'''<br />
|-<br />
| bgcolor="#C3FDB8" | UniProt<br />
| bgcolor="#eeeeee" colspan="2" style="border-top:2px solid #dddddd; border-right:2px solid #dddddd"| <span class=plainlinks>[http://www.uniprot.org/uniprot/P03692 P03692]</span><br />
|-<br />
| bgcolor="#C3FDB8" | EMBL<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01127 V01127]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br />[http://www.ebi.ac.uk/cgi-bin/emblfetch?style=html&Submit=Go&id=V01146 V01146]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PIR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://pir.georgetown.edu/cgi-bin/pirwww/nbrfget?uid=A04314 A04314]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | RefSeq<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041975.1 NP_041975.1]<br />[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NP_041977.1 NP_041977.1]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDB<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR0 1CR0]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR1 1CR1]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR2 1CR2]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CR4 1CR4]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0J 1E0J]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1E0K 1E0K]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NUI 1NUI]<br />[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1Q57 1Q57]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PDBsum<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1CR0<br />1CR1<br />1CR2<br />1CR4<br />1E0J<br />1E0K<br />1NUI<br />1Q57<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | ProteinModelPortal<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMR<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | IntAct<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/intact/search/do/search?searchString=P03692 P03692]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | MINT<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>MINT-1513747<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GeneID<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>1261046<br />1261048<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KEGG<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.genome.ad.jp/kegg/ vg:1261046]<br />[http://www.genome.ad.jp/kegg/ vg:1261048]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | KO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>K17680<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | EvolutionaryTrace<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>P03692<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Proteomes<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>UP000000840<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | GO<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://amigo.geneontology.org/amigo/term/GO:0043139 GO:0043139]<br />[http://amigo.geneontology.org/amigo/term/GO:0005524 GO:0005524]<br />[http://amigo.geneontology.org/amigo/term/GO:0003896 GO:0003896]<br />[http://amigo.geneontology.org/amigo/term/GO:0003697 GO:0003697]<br />[http://amigo.geneontology.org/amigo/term/GO:0008270 GO:0008270]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Gene3D<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://gene3d.biochem.ucl.ac.uk/superfamily/?accession=3.40.50.300 3.40.50.300]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | InterPro<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.ebi.ac.uk/interpro/entry/IPR007694 IPR007694]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027417 IPR027417]<br />[http://www.ebi.ac.uk/interpro/entry/IPR013237 IPR013237]<br />[http://www.ebi.ac.uk/interpro/entry/IPR006171 IPR006171]<br />[http://www.ebi.ac.uk/interpro/entry/IPR027032 IPR027032]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PANTHER<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.pantherdb.org/panther/lookupId.jsp?id=PTHR12873 PTHR12873]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | Pfam<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF03796 PF03796]<br />[http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF08273 PF08273]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SMART<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00778 SM00778]<br />[http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=SM00493 SM00493]<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | SUPFAM<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>SSF52540<br /></span><br />
|-<br />
| bgcolor="#C3FDB8" | PROSITE<br />
|bgcolor='#eeeeee' colspan='2' style='border-top:2px solid #dddddd; border-right:2px solid #dddddd'| <span class=plainlinks>PS51199<br />PS50880<br /></span><br />
<br />
|}<br />
</div><br />
<br />
==Annotations==<br />
<div id="annotations"><br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3--><br />
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I570be2394c9b3" class=" tableEdit Annotation_Headings dataTable" <br />
|- align='left' bgcolor='#CCCCFF'<br />
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status<br />
|- <br />
|<br />
<br />
|<br />
GO:0000166<br />
|<br />
nucleotide binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0547<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003678<br />
|<br />
DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003697<br />
|<br />
single-stranded DNA binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003899<br />
|<br />
DNA-directed RNA polymerase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0240<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0004386<br />
|<br />
helicase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0347<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0005524<br />
|<br />
ATP binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0067<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006260<br />
|<br />
DNA replication<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0235<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0006269<br />
|<br />
DNA replication, synthesis of RNA primer<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0639<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0008270<br />
|<br />
zinc ion binding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR013237<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016740<br />
|<br />
transferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0808<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016779<br />
|<br />
nucleotidyltransferase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0548<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0016787<br />
|<br />
hydrolase activity<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0378<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR007694<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0032508<br />
|<br />
DNA duplex unwinding<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
P<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0043139<br />
|<br />
5'-3' DNA helicase activity<br />
|<br />
GO_REF:0000002<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
InterPro:IPR027032<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0046872<br />
|<br />
metal ion binding<br />
|<br />
GO_REF:0000037<br />
|<br />
IEA: Inferred from Electronic Annotation<br />
|<br />
UniProtKB-KW:KW-0479<br />
|<br />
F<br />
|<br />
Seeded From UniProt<br />
|<br />
<br />
|- <br />
|<br />
<br />
|<br />
GO:0003896<br />
|<br />
DNA primase activity<br />
|<br />
PMID:11279245<br />
|<br />
IDA: Inferred from Direct Assay<br />
|<br />
<br />
|<br />
F<br />
|<br />
Figs. 5, 6. "An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase."<br />
|<br />
complete<br/>[[Special:CACAO/annotation/11625|CACAO 11625]] <br />
|- <br />
|<br />
<br />
|<br />
GO:0003678<br />
|<br />
DNA helicase activity<br />
|<br />
PMID:21606333<br />
|<br />
IMP: Inferred from Mutant Phenotype<br />
|<br />
<br />
|<br />
F<br />
|<br />
Experiment focused on interaction between gene 5 polymerase and gene 4 helicase on DNA synthesis for bacteriophage T7. Showed that replacements of basic residues found on gene 5 disrupts interactions with gene 4, which halted strand-displacement synthesis. This shows that both gene 5 and gene 4 play crucial roles as polymerase and helicase, respectively. See Fig. 2. <br />
|<br />
complete <br />
<br />
|- class="tableEdit_footer" <br />
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3&page=921272&pagename={{FULLPAGENAMEE}}&type=0&template=Annotation_Headings edit table]</div> || || || || || || || ||<br />
|}<br />
<!--box uid=573c5f77b3a094817c4e1c4a6f9b2c99.921272.I570be2394c9b3--><br />
</div><br />
<br />
==Notes==<br />
<br />
<br />
==References==<br />
{{RefHelp}}<br />
<references/><br />
<br />
<br />
[[Category: Viruses]]<br />
[[Category: dsDNA viruses, no RNA stage]]<br />
[[Category: Caudovirales]]<br />
[[Category: Podoviridae]]<br />
[[Category: Autographivirinae]]<br />
[[Category: T7likevirus]]<br />
[[Category: Enterobacteria phage T7]]</div>
AHora