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PMID:2137120

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Citation

Zagotta, MT and Wilson, DB (1990) Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli. J. Bacteriol. 172:912-21

Abstract

Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inner membrane at all times during the lytic cycle. Between 100 and 1,000 molecules of S protein per cell were present at the time of phage-induced lysis. Western blots of chemically cross-linked membranes from induced lysogens showed a ladder of bands at 18, 24, 32, and 42 kilodaltons (the S-protein monomer ran at 8 kilodaltons) that reacted with anti-S-protein antibody. Thus, the S protein appears to reside in the inner membrane as a multimer, and the molecular weights of the cross-linked species are consistent with those of S-protein homopolymers. Sodium dodecyl sulfate-resistant dimers were also detected when S protein was purified by immunoprecipitation.

Links

PubMed PMC208519

Keywords

Amino Acid Sequence; Antibodies; Bacterial Proteins/analysis; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage lambda/genetics; Bacteriophage lambda/growth & development; Bacteriophage lambda/metabolism; Bacteriophages/genetics; Base Sequence; Blotting, Western; Cell Membrane/metabolism; Cross-Linking Reagents; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genotype; Kinetics; Lysogeny; Macromolecular Substances; Membrane Proteins/analysis; Membrane Proteins/biosynthesis; Membrane Proteins/genetics; Molecular Sequence Data; Plasmids; Salmonella typhimurium/genetics; Succinimides

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