GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

9FLAV:Q9J1B7

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 1A.Purification of NS5-interacting proteins using the TAP method

complete
CACAO 8945

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 1B. Western Blot analysis of the purified proteins. Anti-Flag antibody was used to detect Flag-HA-NS5 protein. In lane 1 of both panels, the black arrowheads mark the expected full length Flag-HA-NS5.

complete
CACAO 8946

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 2A. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Hsp70-Myc.

complete
CACAO 8947

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 2C. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Ran-Myc.

complete
CACAO 8948

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 2B.Interaction of the JEV NS5 protein with identified cellular proteins. 293T cells were transfected with expression plasmids encoding Flag-NS5 and eEF-1α-Myc.

complete
CACAO 8950

public

GO:0008166

viral replication

PMID:24086464

ECO:0000315

P

Figure 3B.Interaction of Hsp70 with deletion mutants of NS5 protein. Hsp70-Myc and a series of deletion mutants of Flag-NS5 were co-transfected in 293T cells, precipitated with mouse anti-Flag MAb and then subjected to Western blotting.

complete
CACAO 8951

public

GO:0008166

viral replication

PMID:24086464

ECO:0000315

P

Figure 3C.The purified proteins of GST-NS5 (406-905) and His-Hsp70 were analyzed by SDS-PAGE.

complete
CACAO 8952

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 3D.The direct interaction between GST-NS5 RdRp domain and His-Hsp70 was analyzed by GST pull-down assay and Western blotting.

complete
CACAO 8953

public

GO:0008166

PMID:24086464

ECO:0000314

Figure 4A. Interaction of Hsp70 with components of the JEV replicase complex. Interaction of Hsp70 with JEV NS3. 293T cells were co-transfected with Myc-tagged Hsp70 plasmid and Flag-tagged NS3 plasmid or vector. Cell lysates were harvested 36 h after transfection for co-IP experiments with anti-Flag antibody. The precipitates were then analyzed by Western blotting with anti-Flag or anti-Myc antibody.

complete
CACAO 8954

public

GO:0008166

viral replication

PMID:24086464

ECO:0000314

P

Figure 4 (B) Association of Hsp70 with NS3 and NS5 proteins and viral RNA in JEV-infected cells. 293T cells were transfected with Hsp70-Myc expressing plasmid or vector before being mock-infected or infected with JEV at an MOI of 1.0. Cell lysates were harvested at 36 h p.i. and subjected to immunoprecipitation with antibody against Myc, followed by Western blotting for the detection of NS3 and NS5. The RNA were extracted from the precipitates and then subjected to RT-PCR for detecting viral RNA. RNA extracted from cells infected and uninfected with JEV was used as positive control (PC) and negative control (NC) respectively.

complete
CACAO 8955

protected

involved_in

GO:0001172

transcription, RNA-templated

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0003968

P

Seeded From UniProt

complete

protected

enables

GO:0003968

RNA-directed 5'-3' RNA polymerase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000208

F

Seeded From UniProt

complete

protected

enables

GO:0005524

ATP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000208

F

Seeded From UniProt

complete




Cancel