GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:8226647

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Nagy, PL, McCorkle, GM and Zalkin, H (1993) purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis. J. Bacteriol. 175:7066-73

Abstract

A gene designated purU has been identified and characterized. purU is adjacent to tyrT at min 27.7 on the Escherichia coli chromosome. The gene codes for a 280-amino-acid protein. The C-terminal segment of PurU from residues 84 to 280 exhibits 27% identity with 5'-phosphoribosylglycinamide (GAR) transformylase, the product of purN. Primer extension mapping and assays of lacZ in a promoter probe vector identified two promoters giving mono- and bi-cistronic purU mRNA. Neither mRNA was regulated by purines. Mutations in either of two pairs of genes are required to block synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) from GAR: purN purT (purT encodes an alternative formate-dependent GAR transformylase) or purN purU. On the basis of the growth of purU, purN, and purU purN mutants, it appears that PurU provides the major source of formate for the purT-dependent synthesis of FGAR.

Links

PubMed PMC206834

Keywords

Acyltransferases/biosynthesis; Acyltransferases/genetics; Amidohydrolases/biosynthesis; Amidohydrolases/genetics; Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; Base Sequence; Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor; DNA Primers; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Formates/metabolism; Genes, Bacterial; Genetic Complementation Test; Glycine/analogs & derivatives; Glycine/biosynthesis; Hydroxymethyl and Formyl Transferases; Ligases/biosynthesis; Ligases/genetics; Ligases/metabolism; Molecular Sequence Data; Phosphoribosylglycinamide Formyltransferase; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Ribonucleotides/biosynthesis; Sequence Homology, Amino Acid

public



Cancel