GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
Jin, L, Pahuja, KB, Wickliffe, KE, Gorur, A, Baumgärtel, C, Schekman, R and Rape, M (2012) Ubiquitin-dependent regulation of COPII coat size and function. Nature 482:495-500
Packaging of proteins from the endoplasmic reticulum into COPII vesicles is essential for secretion. In cells, most COPII vesicles are approximately 60-80 nm in diameter, yet some must increase their size to accommodate 300-400 nm procollagen fibres or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII coats have remained elusive. Here, we identified the ubiquitin ligase CUL3-KLHL12 as a regulator of COPII coat formation. CUL3-KLHL12 catalyses the monoubiquitylation of the COPII-component SEC31 and drives the assembly of large COPII coats. As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat.
Animals; COP-Coated Vesicles/chemistry; COP-Coated Vesicles/metabolism; Cell Line; Cell Shape; Collagen/metabolism; Cullin Proteins/genetics; Cullin Proteins/metabolism; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; HeLa Cells; Humans; Mice; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Protein Transport; Ubiquitin/metabolism; Ubiquitination; Vesicular Transport Proteins/genetics; Vesicular Transport Proteins/metabolism