GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Muller, C, Bang, IS, Velayudhan, J, Karlinsey, J, Papenfort, K, Vogel, J and Fang, FC (2009) Acid stress activation of the sigma(E) stress response in Salmonella enterica serovar Typhimurium. Mol. Microbiol. 71:1228-38


The alternative sigma factor sigma(E) is activated by unfolded outer membrane proteins (OMPs) and plays an essential role in Salmonella pathogenesis. The canonical pathway of sigma(E) activation in response to envelope stress involves sequential proteolysis of the anti-sigma factor RseA by the PDZ proteases DegS and RseP. Here we show that sigma(E) in Salmonella enterica sv. Typhimurium can also be activated by acid stress. A sigma(E)-deficient mutant exhibits increased susceptibility to acid pH and reduced survival in an acidified phagosomal vacuole. Acid activation of sigma(E)-dependent gene expression is independent of the unfolded OMP signal or the DegS protease but requires processing of RseA by RseP. The RseP PDZ domain is indispensable for acid induction, suggesting that acid stress may disrupt an inhibitory interaction between RseA and the RseP PDZ domain to allow RseA proteolysis in the absence of antecedent action of DegS. These observations demonstrate a novel environmental stimulus and activation pathway for the sigma(E) regulon that appear to be critically important during Salmonella-host cell interactions.


PubMed Online version:10.1111/j.1365-2958.2009.06597.x


Acids/metabolism; Animals; Bacterial Proteins/metabolism; Cell Line; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Mice; Mutation; RNA, Bacterial/metabolism; Regulon; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Sigma Factor/metabolism; Stress, Physiological; Vacuoles/microbiology