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Dong, Z, Onrust, R, Skangalis, M and O'Donnell, M (1993) DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'. J. Biol. Chem. 268:11758-65


The genes encoding the delta and delta' subunits of the 10-subunit Escherichia coli replicase, DNA polymerase III holoenzyme, have been identified and sequenced. The holA gene encoding delta is located downstream of rlpB at 15.2 min and predicts a 38.7 kda protein. The holB gene encoding delta' is located at 24.3 min and predicts a 36.9-kDa protein. Hence the delta and delta' subunits are unrelated proteins encoded by separate genes. The genes have been used to express and purify delta and delta' in quantity. The predicted amino acid sequence of delta' is homologous to the sequences of the tau and gamma subunits revealing a large amount of structural redundancy within the holoenzyme.




Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Chromatography, Ion Exchange; Chromosome Mapping; Chromosomes, Bacterial; DNA Polymerase III/genetics; DNA Polymerase III/isolation & purification; DNA Polymerase III/metabolism; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Kinetics; Macromolecular Substances; Molecular Sequence Data; Molecular Weight; Oligodeoxyribonucleotides; Open Reading Frames; Plasmids; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Sequence Homology, Amino Acid