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Wovcha, MG, Steuerwald, DL and Brooks, KE (1983) Amplification of D-xylose and D-glucose isomerase activities in Escherichia coli by gene cloning. Appl. Environ. Microbiol. 45:1402-4
A recombinant plasmid, designated pUC1002, was constructed by ligation of a HindIII restriction endonuclease fragment of Escherichia coli chromosomal DNA to vector plasmid pMB9. Strains carrying this plasmid were selected by transformation of an E. coli strain bearing the xyl-7 mutation to a xylose-positive (Xyl+) phenotype. Strains containing pUC1002 produced coordinately elevated levels of D-xylose isomerase and D-xylulose kinase. Under appropriate conditions, the isomerase also efficiently catalyzed the conversion of D-glucose to D-fructose.
Aldose-Ketose Isomerases; Carbohydrate Epimerases/biosynthesis; Carbohydrate Epimerases/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Plasmids