GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Cottier, F, Raymond, M, Kurzai, O, Bolstad, M, Leewattanapasuk, W, Jiménez-López, C, Lorenz, MC, Sanglard, D, Váchová, L, Pavelka, N, Palková, Z and Mühlschlegel, FA (2012) The bZIP transcription factor Rca1p is a central regulator of a novel CO₂ sensing pathway in yeast. PLoS Pathog. 8:e1002485


Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO(2) concentration. This response involves two major proteins: adenylyl cyclase and carbonic anhydrase (CA). Here, we demonstrate that CA expression is tightly controlled by the availability of CO(2) and identify the bZIP transcription factor Rca1p as the first CO(2) regulator of CA expression in yeast. We show that Rca1p upregulates CA expression during contact with mammalian phagocytes and demonstrate that serine 124 is critical for Rca1p signaling, which occurs independently of adenylyl cyclase. ChIP-chip analysis and the identification of Rca1p orthologs in the model yeast Saccharomyces cerevisiae (Cst6p) point to the broad significance of this novel pathway in fungi. By using advanced microscopy we visualize for the first time the impact of CO(2) build-up on gene expression in entire fungal populations with an exceptional level of detail. Our results present the bZIP protein Rca1p as the first fungal regulator of carbonic anhydrase, and reveal the existence of an adenylyl cyclase independent CO(2) sensing pathway in yeast. Rca1p appears to regulate cellular metabolism in response to CO(2) availability in environments as diverse as the phagosome, yeast communities or liquid culture.


PubMed PMC3257301 Online version:10.1371/journal.ppat.1002485


Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Adenosine Triphosphatases/physiology; Basic-Leucine Zipper Transcription Factors/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Basic-Leucine Zipper Transcription Factors/physiology; Biota; Carbon Dioxide/metabolism; Chromatin Immunoprecipitation; Environment; Gene Expression Profiling; Gene Expression Regulation, Fungal; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Metalloendopeptidases/physiology; Microbiological Techniques; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Mitochondrial Proteins/physiology; Models, Biological; Oligonucleotide Array Sequence Analysis; Organisms, Genetically Modified; Phagosomes/genetics; Phagosomes/metabolism; Quorum Sensing/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Signal Transduction/genetics; Signal Transduction/physiology; Yeasts/genetics; Yeasts/metabolism; Yeasts/physiology