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Negoro, M and Wakabayashi, I (2005) Interactions of alcohol dehydrogenase to p-hydroxyacetophenone-sepharose and p-acetamidophenol-sepharose. Alcohol. Clin. Exp. Res. 29:304S-8S
p-Hydroxyacetophenone (HAP)-sepharose is known to be an effective ligand for isolation of aldehyde dehydrogenase and chloramphenicol acetyltransferase. In this study, we investigated ligand specificities to alcohol dehydrogenase (ADH) using p-HAP-sepharose and p-acetamidophenol (AAP)-sepharose.
Acetaminophen/chemistry; Acetophenones/chemistry; Alcohol Dehydrogenase/chemistry; Animals; Chromatography, Affinity; Chromatography, High Pressure Liquid; Cytosol/enzymology; Electrophoresis, Polyacrylamide Gel; Horses; Liver/enzymology; Male; Rats; Rats, Wistar; Sepharose/chemistry; Sequence Analysis, Protein