GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Zhang, H, Zhang, L, Gao, B, Fan, H, Jin, J, Botella, MA, Jiang, L and Lin, J (2011) Golgi apparatus-localized synaptotagmin 2 is required for unconventional secretion in Arabidopsis. PLoS ONE 6:e26477


Most secretory proteins contain signal peptides that direct their sorting to the ER and secreted via the conventional ER/Golgi transport pathway, while some signal-peptide-lacking proteins have been shown to export through ER/Golgi independent secretory pathways. Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus that is active against both prokaryotic and eukaryotic cells. The hygromycin phosphotransferase (HYG(R)) can phosphorylate and inactivate the hygromycin B, and has been widely used as a positive selective marker in the construction of transgenic plants. However, the localization and trafficking of HYG(R) in plant cells remain unknown. Synaptotagmins (SYTs) are involved in controlling vesicle endocytosis and exocytosis as calcium sensors in animal cells, while their functions in plant cells are largely unclear.


PubMed PMC3225361 Online version:10.1371/journal.pone.0026477


Amino Acid Sequence; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/ultrastructure; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/secretion; Brefeldin A/pharmacology; Cell Compartmentation/drug effects; Genes, Plant/genetics; Golgi Apparatus/drug effects; Golgi Apparatus/metabolism; Golgi Apparatus/ultrastructure; Green Fluorescent Proteins/metabolism; Hygromycin B/pharmacology; Models, Biological; Molecular Sequence Data; Mutation/genetics; Protein Transport/drug effects; Recombinant Fusion Proteins/metabolism; Secretory Pathway/drug effects; Subcellular Fractions/drug effects; Subcellular Fractions/metabolism; Synaptotagmin II/chemistry; Synaptotagmin II/genetics; Synaptotagmin II/secretion; Up-Regulation/drug effects