GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:19945479

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Mao, W, Hunt, HD and Cheng, HH (2010) Cloning and functional characterization of chicken stem cell antigen 2. Dev. Comp. Immunol. 34:360-8

Abstract

Stem cell antigen 2 (SCA2) is a Ly6 family member whose function is largely unknown. To characterize biological properties and tissue distribution of chicken SCA2, SCA2 was expressed in E. coli, purified, and a polyclonal antibody developed. Utilizing the polyclonal antibody, SCA2 is a 13 kDa cell surface protein anchored by a glycosyl-phosphatidylinositol (GPI) moiety. SCA2 is expressed in connective tissues of thymus and bursa based on immunohistochemistry, immunoprecipitation, and western blots. In bursal follicles, SCA2 is specifically expressed on the cortical-medullary epithelial cells (CMEC) surrounded by MHC class II presenting cells. Expression profiles of bursal cells induced by contact with SCA2-expressing cells shows down-regulation of numerous genes including CD79B, B cell linker (BLNK), spleen tyrosine kinase (SYK), and gamma 2-phospholipase C (PLCG2) that are involved in the B cell receptor (BCR) and immune response signaling pathways. These results suggest chicken SCA2 plays a role in regulating B lymphocytes.

Links

PubMed Online version:10.1016/j.dci.2009.11.008

Keywords

Amino Acid Sequence; Animals; B-Lymphocytes/immunology; B-Lymphocytes/metabolism; Blotting, Western; Cell Separation; Chickens/genetics; Chickens/immunology; Chickens/metabolism; Cloning, Molecular; Flow Cytometry; Gene Expression Regulation; Immunohistochemistry; Immunoprecipitation; Membrane Proteins/genetics; Membrane Proteins/immunology; Membrane Proteins/metabolism; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction/immunology

public



Cancel