GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Dave, RK, Hume, DA, Elsegood, C and Kellie, S (2009) CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages. Exp. Cell Res. 315:1734-44


In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity.


PubMed Online version:10.1016/j.yexcr.2009.02.023


Actins/metabolism; Animals; Antibodies, Monoclonal/pharmacology; Cell Proliferation/drug effects; Cell Surface Extensions/drug effects; Cell Surface Extensions/enzymology; Cell Survival/drug effects; Cells, Cultured; Chemotaxis/drug effects; Cross-Linking Reagents/pharmacology; Cytoskeleton/drug effects; Cytoskeleton/enzymology; Endocytosis/drug effects; Humans; Lipopolysaccharides/pharmacology; Macrophage Colony-Stimulating Factor/pharmacology; Macrophages/cytology; Macrophages/drug effects; Macrophages/enzymology; Mice; Mice, Inbred C57BL; Phosphorylation/drug effects; Protein Binding/drug effects; Protein Transport/drug effects; Receptor, Macrophage Colony-Stimulating Factor/metabolism; Receptor-Like Protein Tyrosine Phosphatases, Class 3/metabolism