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PMID:18782221

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Citation

Nishikori, S, Yamanaka, K, Sakurai, T, Esaki, M and Ogura, T (2008) p97 Homologs from Caenorhabditis elegans, CDC-48.1 and CDC-48.2, suppress the aggregate formation of huntingtin exon1 containing expanded polyQ repeat. Genes Cells 13:827-38

Abstract

Polyglutamine (polyQ)-expanded proteins are associated with cytotoxicity in some neurodegenerative disorders such as Huntington's disease. We have reported that the aggregation of the polyQ-expanded protein is partially suppressed by co-expression of either of two homologs of an AAA chaperone p97, CDC-48.1 or CDC-48.2, in Caenorhabditis elegans, but how p97 regulates the aggregation of polyQ-expanded proteins remains unclear. Here we present direct evidence that CDC-48.1 and CDC-48.2 suppress the aggregation of a huntingtin (Htt) exon1 fragment containing an expanded polyQ repeat in vitro. CDC-48.1 and CDC-48.2 bound the Htt exon1 fragment directly, and suppressed the formation of SDS-insoluble aggregates of Htt fragments containing 53 glutamine residues (HttQ53) independently of nucleotides. CDC-48.1 and CDC-48.2 also modulated the oligomeric states of HttQ53 during the aggregate formation. In the absence of CDC-48.1 and CDC-48.2, HttQ53 formed 70-150 kDa oligomers, whereas 300-500 kDa oligomers as well as 70-150 kDa oligomers accumulated in the presence of CDC-48.1 and CDC-48.2. Taken together, these results suggest that p97 plays a protective role in neurodegenerative disorders by directly suppressing the protein aggregation as a molecular chaperone.

Links

PubMed Online version:10.1111/j.1365-2443.2008.01214.x

Keywords

Adenosine Triphosphatases/metabolism; Animals; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/metabolism; Cattle; Cell Cycle Proteins/metabolism; Exons; Molecular Chaperones/metabolism; Nerve Tissue Proteins/metabolism; Neurodegenerative Diseases/metabolism; Nuclear Proteins/metabolism; Thiosulfate Sulfurtransferase/metabolism

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