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Lucker, BF, Miller, MS, Dziedzic, SA, Blackmarr, PT and Cole, DG (2010) Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46. J. Biol. Chem. 285:21508-18
Intraflagellar transport (IFT) particles of Chlamydomonas reinhardtii contain two distinct protein complexes, A and B, composed of at least 6 and 15 protein subunits, respectively. As isolated from C. reinhardtii flagella, IFT complex B can be further reduced to a approximately 500-kDa core that contains IFT88, 2x IFT81, 2x IFT74/72, IFT52, IFT46, IFT27, IFT25, and IFT22. In this study, yeast-based two-hybrid analysis was combined with bacterial coexpression to show that three of the core B subunits, IFT88, IFT52, and IFT46, interact directly with each other and, together, are capable of forming a ternary complex. Chemical cross-linking results support the IFT52-IFT88 interaction and provide additional evidence of an association between IFT27 and IFT81. With previous studies showing that IFT81 and IFT74/72 interact to form a (IFT81)(2)(IFT74/72)(2) heterotetramer and that IFT27 and IFT25 form a heterodimer, the architecture of complex B is revealing itself. Last, electroporation of recombinant IFT46 was used to rescue flagellar assembly of a newly identified ift46 mutant and to monitor in vivo localization and movement of the IFT particles.
Algal Proteins/metabolism; Biological Transport; Carrier Proteins/metabolism; Chlamydomonas reinhardtii/genetics; Chlamydomonas reinhardtii/metabolism; Cross-Linking Reagents/chemistry; Electroporation; Flagella/metabolism; Gene Expression Regulation; Models, Biological; Mutagenesis; Phenotype; Plasmids/metabolism; Protein Binding; Protozoan Proteins/metabolism; Recombinant Proteins/chemistry; Two-Hybrid System Techniques