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Dean, D, Yates, JL and Nomura, M (1981) Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli. Cell 24:413-9


A DNA-directed in vitro protein-synthesizing system was used to demonstrate that r protein S7 has the capacity to inhibit the translation of mRNA for the second and third gene products of the str operon (S7 and EF-G) but not for the first gene product (S12). Translation of mRNA of the last gene product in the operon (EF-Tu) is also probably not inhibited by S7. In addition, we localized the target site for S7 repressor action on the polycistronic str mRNA by examining the repressor activity of S7 in vitro using various template DNAs that contain the gene. The target site was found not to include a promoter-proximal portion of the mRNA for S12. To test for regulatory properties of S7 in vivo, we inserted the S7 gene into a plasmid vector containing the ara regulatory elements such that S7 synthesis was placed under ara control. A specific increase in S7 synthesis caused by stimulation in transcription originating from the arabinose promoter decreased the synthetic rate for EF-G but had no effect on S12 or EF-Tu synthesis.




Bacterial Proteins/genetics; Escherichia coli/genetics; Feedback; Genes; Operon; Protein Biosynthesis; RNA, Messenger/genetics; Repressor Proteins/genetics; Ribosomal Proteins/genetics; Streptomycin; Transcription Factors/genetics