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Fujimura, K, Tanaka, K, Nakano, A and Toh-e, A (1994) The Saccharomyces cerevisiae MSI4 gene encodes the yeast counterpart of component A of Rab geranylgeranyltransferase. J. Biol. Chem. 269:9205-12


We describe the isolation of MSI4 as a multicopy suppressor of ira1 (inhibitory regulator of Ras). We find it encodes a 66-kDa protein with three regions similar to component A of Rab geranylgeranyltransferase and Rab guanine nucleotide dissociation inhibitor. A gene disruption experiment showed that MSI4 is essential for cell growth. To investigate its functions further, we constructed a strain whose MSI4 is driven by the GAL1 promoter. This strain accumulated the endoplasmic reticulum precursor form of a vacuolar enzyme, carboxypeptidase Y, under the restrictive conditions. Moreover, we showed that the activity of geranylgeranyl-transferase for Ypt1p was very low in the mutant shifted to glucose medium. We propose that the MSI4 gene product is a yeast counterpart of component A of Rab geranylgeranyltransferase that is essential for Ypt1p to localize on membranes.




Adaptor Proteins, Signal Transducing; Alkyl and Aryl Transferases; Amino Acid Sequence; Base Sequence; Biological Transport; Carrier Proteins/genetics; Cell Compartmentation; Choroideremia/genetics; DNA, Fungal/genetics; GTP-Binding Proteins/metabolism; Genes, Fungal; Genes, Suppressor; Molecular Sequence Data; Mutagenesis, Insertional; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Deletion; Sequence Homology, Amino Acid; Transferases/genetics; rab GTP-Binding Proteins