GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Laroia, G, Cuesta, R, Brewer, G and Schneider, RJ (1999) Control of mRNA decay by heat shock-ubiquitin-proteasome pathway. Science 284:499-502


Cytokine and proto-oncogene messenger RNAs (mRNAs) are rapidly degraded through AU-rich elements in the 3' untranslated region. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat shock proteins hsc70-hsp70, translation initiation factor eIF4G, and poly(A) binding protein. AU-rich mRNA decay is associated with displacement of eIF4G from AUF1, ubiquitination of AUF1, and degradation of AUF1 by proteasomes. Induction of hsp70 by heat shock, down-regulation of the ubiquitin-proteasome network, or inactivation of ubiquitinating enzyme E1 all result in hsp70 sequestration of AUF1 in the perinucleus-nucleus, and all three processes block decay of AU-rich mRNAs and AUF1 protein. These results link the rapid degradation of cytokine mRNAs to the ubiquitin-proteasome pathway.




3' Untranslated Regions; Carrier Proteins/metabolism; Cell Nucleus/metabolism; Cysteine Endopeptidases/metabolism; Cysteine Proteinase Inhibitors/pharmacology; Cytoplasm/metabolism; Eukaryotic Initiation Factor-4G; Granulocyte-Macrophage Colony-Stimulating Factor/genetics; HSC70 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/metabolism; HeLa Cells; Heat-Shock Response; Heterogeneous-Nuclear Ribonucleoprotein D; Humans; Leupeptins/pharmacology; Multienzyme Complexes/metabolism; Peptide Initiation Factors/metabolism; Poly(A)-Binding Proteins; Proteasome Endopeptidase Complex; Protein Binding; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA-Binding Proteins/metabolism; Transfection; Ubiquitins/metabolism