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Liu, LX, Margottin, F, Le Gall, S, Schwartz, O, Selig, L, Benarous, R and Benichou, S (1997) Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation. J. Biol. Chem. 272:13779-85


Nef is a 27-kDa myristoylated protein conserved in primate lentiviruses. In vivo, simian immunodeficiency virus Nef is required in macaques to produce a high viral load and full pathological effects. Nef has at least three major effects in vitro, induction of CD4 down-regulation, alteration of T cell activation pathways, and enhancement of viral infectivity. We have used the yeast two-hybrid system to identify cellular proteins that interact with HIV-1Lai Nef and could mediate Nef function. A human cDNA was isolated that encodes a new type of thioesterase, an enzyme that cleaves thioester bonds. This novel thioesterase is unlike the animal types I and II thioesterases previously cloned but is homologous to the Escherichia coli thioesterase II. Nef and this thioesterase interact in vitro and are co-immunoprecipitated by anti-Nef antibodies in CEM cells expressing Nef. Nef alleles from human immunodeficiency virus-1 (HIV-1) isolates unable to down-regulate CD4 do not react or react poorly with thioesterase. An HIV-1 NefLai mutant selected for its lack of interaction with thioesterase was also unable to down-regulate CD4 cell-surface expression. These observations suggest that this human thioesterase is a cellular mediator of Nef-induced CD4 down-regulation.




Alleles; Amino Acid Sequence; Antigens, CD4/metabolism; Cloning, Molecular; DNA, Complementary/chemistry; Down-Regulation; Escherichia coli/enzymology; Fatty Acid Synthetase Complex/genetics; Fatty Acid Synthetase Complex/metabolism; Gene Products, nef/metabolism; HIV-1; Humans; Jurkat Cells; Molecular Sequence Data; Palmitoyl-CoA Hydrolase; Proteins; Recombinant Proteins/metabolism; T-Lymphocytes/metabolism; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism; nef Gene Products, Human Immunodeficiency Virus