GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:9153233
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Liu, LX, Margottin, F, Le Gall, S, Schwartz, O, Selig, L, Benarous, R and Benichou, S (1997) Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation. J. Biol. Chem. 272:13779-85 |
|---|---|
| Abstract |
Nef is a 27-kDa myristoylated protein conserved in primate lentiviruses. In vivo, simian immunodeficiency virus Nef is required in macaques to produce a high viral load and full pathological effects. Nef has at least three major effects in vitro, induction of CD4 down-regulation, alteration of T cell activation pathways, and enhancement of viral infectivity. We have used the yeast two-hybrid system to identify cellular proteins that interact with HIV-1Lai Nef and could mediate Nef function. A human cDNA was isolated that encodes a new type of thioesterase, an enzyme that cleaves thioester bonds. This novel thioesterase is unlike the animal types I and II thioesterases previously cloned but is homologous to the Escherichia coli thioesterase II. Nef and this thioesterase interact in vitro and are co-immunoprecipitated by anti-Nef antibodies in CEM cells expressing Nef. Nef alleles from human immunodeficiency virus-1 (HIV-1) isolates unable to down-regulate CD4 do not react or react poorly with thioesterase. An HIV-1 NefLai mutant selected for its lack of interaction with thioesterase was also unable to down-regulate CD4 cell-surface expression. These observations suggest that this human thioesterase is a cellular mediator of Nef-induced CD4 down-regulation. |
| Links | |
| Keywords |
Alleles; Amino Acid Sequence; Antigens, CD4/metabolism; Cloning, Molecular; DNA, Complementary/chemistry; Down-Regulation; Escherichia coli/enzymology; Fatty Acid Synthetase Complex/genetics; Fatty Acid Synthetase Complex/metabolism; Gene Products, nef/metabolism; HIV-1; Humans; Jurkat Cells; Molecular Sequence Data; Palmitoyl-CoA Hydrolase; Proteins; Recombinant Proteins/metabolism; T-Lymphocytes/metabolism; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism; nef Gene Products, Human Immunodeficiency Virus |
| public |
| Cancel |
