GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:18215111

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Thomas, CL, Bayer, EM, Ritzenthaler, C, Fernandez-Calvino, L and Maule, AJ (2008) Specific targeting of a plasmodesmal protein affecting cell-to-cell communication. PLoS Biol. 6:e7

Abstract

Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non-cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plant biology. Features of protein sequences and/or structure that determine protein targeting to plasmodesmata were previously unknown. We identify here a novel family of plasmodesmata-located proteins (called PDLP1) whose members have the features of type I membrane receptor-like proteins. We focus our studies on the first identified type member (namely At5g43980, or PDLP1a) and show that, following its altered expression, it is effective in modulating cell-to-cell trafficking. PDLP1a is targeted to plasmodesmata via the secretory pathway in a Brefeldin A-sensitive and COPII-dependent manner, and resides at plasmodesmata with its C-terminus in the cytoplasmic domain and its N-terminus in the apoplast. Using a deletion analysis, we show that the single transmembrane domain (TMD) of PDLP1a contains all the information necessary for intracellular targeting of this type I membrane protein to plasmodesmata, such that the TMD can be used to target heterologous proteins to this location. These studies identify a new family of plasmodesmal proteins that affect cell-to-cell communication. They exhibit a mode of intracellular trafficking and targeting novel for plant biology and provide technological opportunities for targeting different proteins to plasmodesmata to aid in plasmodesmal characterisation.

Links

PubMed PMC2211546 Online version:10.1371/journal.pbio.0060007

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/classification; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/classification; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Communication; Extracellular Matrix/metabolism; Molecular Sequence Data; Mutation/genetics; Phylogeny; Plasmodesmata/metabolism; Protein Transport; Sequence Alignment; Signal Transduction

public



Cancel