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Lanahan, A, Zhang, X, Fantin, A, Zhuang, Z, Rivera-Molina, F, Speichinger, K, Prahst, C, Zhang, J, Wang, Y, Davis, G, Toomre, D, Ruhrberg, C and Simons, M (2013) The neuropilin 1 cytoplasmic domain is required for VEGF-A-dependent arteriogenesis. Dev. Cell 25:156-68


Neuropilin 1 (NRP1) plays an important but ill-defined role in VEGF-A signaling and vascular morphogenesis. We show that mice with a knockin mutation that ablates the NRP1 cytoplasmic tail (Nrp1(cyto)) have normal angiogenesis but impaired developmental and adult arteriogenesis. The arteriogenic defect was traced to the absence of a PDZ-dependent interaction between NRP1 and VEGF receptor 2 (VEGFR2) complex and synectin, which delayed trafficking of endocytosed VEGFR2 from Rab5+ to EAA1+ endosomes. This led to increased PTPN1 (PTP1b)-mediated dephosphorylation of VEGFR2 at Y(1175), the site involved in activating ERK signaling. The Nrp1(cyto) mutation also impaired endothelial tubulogenesis in vitro, which could be rescued by expressing full-length NRP1 or constitutively active ERK. These results demonstrate that the NRP1 cytoplasmic domain promotes VEGFR2 trafficking in a PDZ-dependent manner to regulate arteriogenic ERK signaling and establish a role for NRP1 in VEGF-A signaling during vascular morphogenesis.


PubMed PMC3774154 Online version:10.1016/j.devcel.2013.03.019


Animals; Arteries/cytology; Arteries/physiopathology; Cells, Cultured; Cytoplasm/metabolism; Endocytosis/physiology; Endosomes/metabolism; Endothelium, Vascular/cytology; Endothelium, Vascular/metabolism; MAP Kinase Signaling System; Mice; Morphogenesis/physiology; Neovascularization, Pathologic; Neuropilin-1/physiology; Phosphorylation; Signal Transduction; Transferrin/metabolism; Vascular Endothelial Growth Factor A/genetics; Vascular Endothelial Growth Factor A/metabolism; Vascular Endothelial Growth Factor Receptor-2/genetics; Vascular Endothelial Growth Factor Receptor-2/metabolism; Vesicular Transport Proteins/metabolism