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BPSPP:HOL24

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0020002

host cell plasma membrane

PMID:27825035

ECO:0000314

C

In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp 24.1 shows that the holin-like protein of Bacillus phage SPP1 was detected at much higher levels in the cell membrane after cell fractionation, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp24.1, with no band visible in the cytoplasmic fraction.

complete
CACAO 12664

public

GO:0033644

host cell membrane

PMID:27825035

ECO:0000315

C

Figure 3C illustrates the alignment of nucleotide sequence of the early promoters PE1, PE2,and PE3 in comparison to that of PE5 in the SPP1 genome sequence. As the cell grows the B.subtilis in seen to not have an effect on its growth. The occurrence of holin had little effect on the development of the cell.

complete
CACAO 12891

public

GO:0039662

host cell outer membrane

PMID:27825035

ECO:0000314

C

Bacillus phage SPP1 (Bacteriophage SPP1) holin-like protein 24.1 (gp24.1) As shown in Figure 4, the holin-like protein gp24.1 localizes to the cytosplasmic membrane.

complete

protected

part_of

GO:0020002

host cell plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1032
UniProtKB-KW:KW-1030
UniProtKB-SubCell:SL-0373

C

Seeded From UniProt

complete

protected

part_of

GO:0016021

integral component of membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

protected

involved_in

GO:0019835

cytolysis

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0204

P

Seeded From UniProt

complete

protected

part_of

GO:0033644

host cell membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1043

C

Seeded From UniProt

complete

protected

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete




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