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BPP22:NEEDL

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0019073

viral DNA genome packaging

PMID:6363718

ECO:0000315

P

Fro. I. Dependence of phage formation in vitro on the concentration of the capsid donor extract. Infected cell[ extracts containing incomplete capsids were freshly thawed and lysed as described in Materials and Methods. Diluted .samples were mixed with concentrated protein donor extract {5-) and incubated for I h at 23°C, at which time the reactions were stopped by further dilution and titered for viable phage. The background of the protein donor extract was 102 phage/mi. The background of the • at mo.~l, Conc-entrated calmid donor extracts were (0) 3.3× 10 (4- extract), (t) 2:5 × l0 T (10- extract) and (at) 2 x 103 (26-extract).

Dependence of in vitro assembly on the concentration of the 4", lO ÷ and 26* activities. (a)

Complementation of 10- extracts (O), 26- extracts (11) and 4- extracts (~lk) with protein donor extract. The capsid extracts were diluted 25-fold, to a final infected cell conventration of about 2 x 10 ° c~lls/ml. Samples were mixed with serial dilutions of the 5- protein donor. The maximum slo|~s determined from these curve,a were: 4+ activity, 5 to 6; 26 + activity. I to 2; 10 + activity, 4 to 5. The background of the undiluted extracts were protein donor 10a; 4- extract < 10s; 10- extracts, 5 x 10~; 26- extract, 2 x l0 a. (b) Variation of the ratio of cap`aid,a to protein; complemcntation of 4- capsids with cap,aid donor cell concentration: (O) 5x lO 9 cell,a/ml; (A) 2 x l0 s cell,a/ml; (m) 4 x 10 v (~lls/ml. The protein donor extract was from the same batch a.s used in (a).

complete
CACAO 12034

protected

involved_in

GO:0099002

viral genome ejection through host cell envelope, short tail mechanism

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1244

P

Seeded From UniProt

complete

protected

part_of

GO:0098015

virus tail

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1227

C

Seeded From UniProt

complete

protected

involved_in

GO:0046718

viral entry into host cell

GO_REF:0000037
GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1162
UniProtKB-KW:KW-1160

P

Seeded From UniProt

complete

protected

involved_in

GO:0044694

pore-mediated entry of viral genome into host cell

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1172

P

Seeded From UniProt

complete

protected

part_of

GO:0019012

virion

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0946
UniProtKB-SubCell:SL-0274

C

Seeded From UniProt

complete




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