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Corn, JE, Pease, PJ, Hura, GL and Berger, JM (2005) Crosstalk between primase subunits can act to regulate primer synthesis in trans. Mol. Cell 20:391-401
The coordination of primase function within the replisome is an essential but poorly understood feature of lagging strand synthesis. By using crystallography and small-angle X-ray scattering (SAXS), we show that functional elements of bacterial primase transition between two dominant conformations: an extended form that uncouples a regulatory domain from its associated RNA polymerase core and a compact state that sequesters the regulatory region from the site of primer synthesis. FRET studies and priming assays reveal that the regulatory domain of one primase subunit productively associates with nucleic acid that is bound to the polymerase domain of a second protomer in trans. This intersubunit interaction allows primase to select initiation sites on template DNA and implicates the regulatory domain as a "molecular brake" that restricts primer length. Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis.
Bacteriophage T7/enzymology; Crystallography, X-Ray/methods; DNA/chemistry; DNA/metabolism; DNA Primase/chemistry; DNA Primase/metabolism; DNA Replication/physiology; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Oligoribonucleotides/biosynthesis; Oligoribonucleotides/chemistry; Protein Structure, Tertiary; Viral Proteins/chemistry; Viral Proteins/metabolism