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sdhA is a gene that encodes for succinate dehydrogenase. Succinate dehydrogenase is an enzyme that contains iron, and it's transcriptional levels are based on iron availability. NrrF is a small RNA present that regulates this expression dependent upon the iron available in the environment. This is represented by Figure 4B and 4C. The figure shows the RNA levels of the sdhA gene present inside a wild-type and NrrF mutant in both iron-rich and iron-depleted environments at different times after innoculation.

Figure 5. Induction of the CWSS is dependent upon a two-component system, vraR and vraS. This system is activated in response to cell wall damage or distress. Mutant phenotypes had deletions in the CWSS genes and was not able to be induced. This left the cell susceptible to the antibiotics because vraRS also have intrinsic resistance/tolerance towards them.

The saeS gene is part of a two-component system [saeRS] that is responsible for several functions. Among them, the virulence factor is increased with induction. [Figure 1A]. The expression levels of the specific proteins are shown on the SDS-PAGE in the wild type and mutated Newman strains [Figure 1B]. It shows that a deletion in the sae gene results in little to no expression of the proteins, such as Eap. Expression of these proteins to increase virulence is dependent upon the sae system.

Figure 6 and Table 3. Both show that the dnaK mutants had a maximum growth rate lower than the wild type strain at various stresses (temperature, pH, etc.)

Figure 5. The strain MC58 with a wild-type recB allele showed to be less sensitive to the UV254 radiation than the strains MC58-1 and MC58-2 with a defective recB allele. This is represented by the different survival rates shown on Fig. 5b.