| HUMAN:DPP10 | 2012-02-12 04:27:pm CST | GO:0090004 - positive regulation of establishment of protein localization in plasma membrane (P) | PMID:15671030 | IDA | Figure 1d showed that DPP10 facilitated cell membrane surface expression of Kv4.2 protein through in situ hybridization.
accession #:AY172661
| challenge |
| RAT:DPP6 | 2012-02-07 07:10:pm CST | GO:0090004 - positive regulation of establishment of protein localization in plasma membrane (P) | PMID:15671030 | IDA | figure 1c shows that DPPX (DPP6) promoted a robust trafficking of Kv4.2 to the plasma membrane.
accession #:M76427
| challenge |
| HORVU:IAA1 | 2012-02-07 07:11:pm CST | GO:0015066 - alpha-amylase inhibitor activity (F) | PMID:2785932 | IDA | | challenge |
| ORYSJ:SLR1 | 2012-02-07 07:33:pm CST | GO:0005634 - nucleus (C) | PMID:10713441 | IDA | Results section 3.5 and Figure 5 detail that nucleus localization occurs because of a bipartite NLS in the protein. Epifluorescence microscopy was used to determine the localization pattern.
| challenge |
| MOUSE:LBH | 2012-02-12 04:25:pm CST | GO:0005634 - nucleus (C) | PMID:11336496 | IDA | Section of the text labeled "Biochemical Characterization of Lbh protein" refers to N and C terminus labeling using an antibody to detect localization of the Lbh protein in the nucleus. (figure 2b)
| challenge |
| HUMAN:CRCM | 2012-02-12 05:24:pm CST | GO:0005737 - cytoplasm (C) | PMID:18591935 | IDA | Figure 1c showed Western Blot Analysis was used to confirm localization of the MCC protein to the cytoplasm.
| challenge |
| HUMAN:CRCM | 2012-02-12 05:24:pm CST | GO:0005634 - nucleus (C) | PMID:18591935 | IDA | Figure 1c showed the use of Western Blot Analysis to confirm MCC protein localization in the nucleus of cells.
| challenge |
| HUMAN:CRCM | 2012-02-12 05:24:pm CST | GO:0051726 - regulation of cell cycle (P) | PMID:18591935 | IDA | Figure 4C and 4D shows that MCC regulates the cell cycle by suppressing the DNA-binding activity of β-catenin/TCF/LEF in the nucleus through Western Blot Analysis. This is also confirmed in the section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels".
| challenge |
| HUMAN:CRCM | 2012-02-12 05:24:pm CST | GO:0045184 - establishment of protein localization (P) | PMID:18591935 | IDA | The section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels" confirms that MCC relocalizes the β-catenin to the cytoplasm. The results are further confirmed through Western Blot Analysis in Figure 4.
| challenge |
| HUMAN:CRCM | 2012-02-12 03:50:pm CST | GO:0030308 - negative regulation of cell growth (P) | PMID:18591935 | IDA | Immunofluorescence was used and confirmed in Figure 6A/B to show that MCC is responsible for the suppression of basal cell growth.
| challenge |
| SHEEP:UTMP | 2012-02-12 04:22:pm CST | GO:0030414 - peptidase inhibitor activity (F) | PMID:8942989 | IDA | Figure 2.a/b show evidence of %Pepsin activity severely decreased between pH of 2-4.5. Supported text for the experiment is listed under "Inhibitory Activities of Uterine Secretions" with standard assay.
| challenge |
| HUMAN:DPP2 | 2012-03-27 08:13:pm CDT | GO:0031982 - vesicle (C) | PMID:11067927 | IDA | figure 5 shows through confocal microscopy, localization of QPP (DPP2) to the vesicles.
| challenge |
| STRPN:ZMPB | 2012-03-27 08:34:pm CDT | GO:0009986 - cell surface (C) | PMID:10792723 | IDA | figure 1b shows through immunoelectron microscopy, that ZmpB localizes to the cell surface.
| challenge |
| GLOHA:VMHA | 2012-03-27 08:45:pm CDT | GO:0072577 - endothelial cell apoptosis (P) | PMID:14688240 | IDA | Figure 7 shows endothelial cell apoptosis caused by halysase through fluorescence.
| challenge |
| MOUSE:CSEN | 2012-03-31 11:08:am CDT | GO:0017163 - basal transcription repressor activity (F) | PMID:11792319 | IDA | Figure 6 shows that DREAM represses basal prodynorphin expression in the spinal chord.
| challenge |
| ARATH:AUX1 | 2012-04-10 07:59:pm CDT | GO:0048767 - root hair elongation (P) | PMID:10036773 | IDA | figures 4 and 5 show that AUX1 causes root hair development over time through microscopy.
| challenge |
| ARATH:AUX1 | 2012-04-10 08:18:pm CDT | GO:0009958 - positive gravitropism (P) | PMID:10205161 | EXP | figure 3 shows that AUX1 restores gravitropic root growth.
| challenge |
| ARATH:IAA7 | 2012-04-10 08:24:pm CDT | GO:0009959 - negative gravitropism (P) | PMID:10205161 | IGI | figure 4 shows that axr2 has an agravitropic affect on root growth through a rescue experiment.
| challenge |
| ARATH:IAA17 | 2012-04-10 08:26:pm CDT | GO:0009959 - negative gravitropism (P) | PMID:10205161 | IGI | figure 4 shows that axr3 has an agravitropic effect on root growth.
| challenge |
| HUMAN:SYNE2 | 2012-04-14 11:40:am CDT | GO:0005634 - nucleus (C) | PMID:12118075 | IDA | Figure 5 shows that NUANCE localizes to the nucleus through immunolabeling.
| challenge |
| HUMAN:SYNE2 | 2012-04-14 11:42:am CDT | GO:0005737 - cytoplasm (C) | PMID:12118075 | IDA | Figure 5 shows that NUANCE can localize to the cytoplasm through immunolabeling.
| challenge |