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StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
HUMAN:DPP102012-02-12 04:27:pm CSTGO:0090004 - positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030IDA

Figure 1d showed that DPP10 facilitated cell membrane surface expression of Kv4.2 protein through in situ hybridization.

accession #:AY172661

challenge
RAT:DPP62012-02-07 07:10:pm CSTGO:0090004 - positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030IDA

figure 1c shows that DPPX (DPP6) promoted a robust trafficking of Kv4.2 to the plasma membrane.

accession #:M76427

challenge
HORVU:IAA12012-02-07 07:11:pm CSTGO:0015066 - alpha-amylase inhibitor activity (F)PMID:2785932IDA

Table 1

challenge
ORYSJ:SLR12012-02-07 07:33:pm CSTGO:0005634 - nucleus (C)PMID:10713441IDA

Results section 3.5 and Figure 5 detail that nucleus localization occurs because of a bipartite NLS in the protein. Epifluorescence microscopy was used to determine the localization pattern.

challenge
MOUSE:LBH2012-02-12 04:25:pm CSTGO:0005634 - nucleus (C)PMID:11336496IDA

Section of the text labeled "Biochemical Characterization of Lbh protein" refers to N and C terminus labeling using an antibody to detect localization of the Lbh protein in the nucleus. (figure 2b)

challenge
HUMAN:CRCM2012-02-12 05:24:pm CSTGO:0005737 - cytoplasm (C)PMID:18591935IDA

Figure 1c showed Western Blot Analysis was used to confirm localization of the MCC protein to the cytoplasm.

challenge
HUMAN:CRCM2012-02-12 05:24:pm CSTGO:0005634 - nucleus (C)PMID:18591935IDA

Figure 1c showed the use of Western Blot Analysis to confirm MCC protein localization in the nucleus of cells.

challenge
HUMAN:CRCM2012-02-12 05:24:pm CSTGO:0051726 - regulation of cell cycle (P)PMID:18591935IDA

Figure 4C and 4D shows that MCC regulates the cell cycle by suppressing the DNA-binding activity of β-catenin/TCF/LEF in the nucleus through Western Blot Analysis. This is also confirmed in the section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels".

challenge
HUMAN:CRCM2012-02-12 05:24:pm CSTGO:0045184 - establishment of protein localization (P)PMID:18591935IDA

The section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels" confirms that MCC relocalizes the β-catenin to the cytoplasm. The results are further confirmed through Western Blot Analysis in Figure 4.

challenge
HUMAN:CRCM2012-02-12 03:50:pm CSTGO:0030308 - negative regulation of cell growth (P)PMID:18591935IDA

Immunofluorescence was used and confirmed in Figure 6A/B to show that MCC is responsible for the suppression of basal cell growth.

challenge
SHEEP:UTMP2012-02-12 04:22:pm CSTGO:0030414 - peptidase inhibitor activity (F)PMID:8942989IDA

Figure 2.a/b show evidence of %Pepsin activity severely decreased between pH of 2-4.5. Supported text for the experiment is listed under "Inhibitory Activities of Uterine Secretions" with standard assay.

challenge
HUMAN:DPP22012-03-27 08:13:pm CDTGO:0031982 - vesicle (C)PMID:11067927IDA

figure 5 shows through confocal microscopy, localization of QPP (DPP2) to the vesicles.

challenge
STRPN:ZMPB2012-03-27 08:34:pm CDTGO:0009986 - cell surface (C)PMID:10792723IDA

figure 1b shows through immunoelectron microscopy, that ZmpB localizes to the cell surface.

challenge
GLOHA:VMHA2012-03-27 08:45:pm CDTGO:0072577 - endothelial cell apoptosis (P)PMID:14688240IDA

Figure 7 shows endothelial cell apoptosis caused by halysase through fluorescence.

challenge
MOUSE:CSEN2012-03-31 11:08:am CDTGO:0017163 - basal transcription repressor activity (F)PMID:11792319IDA

Figure 6 shows that DREAM represses basal prodynorphin expression in the spinal chord.

challenge
ARATH:AUX12012-04-10 07:59:pm CDTGO:0048767 - root hair elongation (P)PMID:10036773IDA

figures 4 and 5 show that AUX1 causes root hair development over time through microscopy.

challenge
ARATH:AUX12012-04-10 08:18:pm CDTGO:0009958 - positive gravitropism (P)PMID:10205161EXP

figure 3 shows that AUX1 restores gravitropic root growth.

challenge
ARATH:IAA72012-04-10 08:24:pm CDTGO:0009959 - negative gravitropism (P)PMID:10205161IGI

figure 4 shows that axr2 has an agravitropic affect on root growth through a rescue experiment.

challenge
ARATH:IAA172012-04-10 08:26:pm CDTGO:0009959 - negative gravitropism (P)PMID:10205161IGI

figure 4 shows that axr3 has an agravitropic effect on root growth.

challenge
HUMAN:SYNE22012-04-14 11:40:am CDTGO:0005634 - nucleus (C)PMID:12118075IDA

Figure 5 shows that NUANCE localizes to the nucleus through immunolabeling.

challenge
HUMAN:SYNE22012-04-14 11:42:am CDTGO:0005737 - cytoplasm (C)PMID:12118075IDA

Figure 5 shows that NUANCE can localize to the cytoplasm through immunolabeling.

challenge
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