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User:Dahle001

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableSTAAU:MECR2012-02-23 12:25:23 CSTGO:0016481 negative regulation of transcription (1)PMID:22295022IDA

Figure 1, part A1

challenge
requireschangesHUMAN:ATF42012-03-12 15:37:31 CDTGO:0010467 gene expression (P)PMID:22363646IDA

Figure 1A: Protein and mRNA levels of ATF4 were determined using Western Blot and qPCR. β-actin and GAPDH were used as a control.

challenge
requireschangesHUMAN:ATF42012-03-12 15:58:54 CDTGO:0001558 regulation of cell growth (P)PMID:22363646IMP

Figure 1B, C, D, E, H: When transfected into SGC7901 and AGS cells, colony number increased due to overexpression of ATF4. When ATF4 was knocked out of expression in SGC7901/ADR and SGC7901/VCR cells the colony number decreased. This shows that ATF4 produced multi-drug resistance in cancer cells, allowing for cell growth.

challenge
requireschangesHUMAN:SIRT12012-03-13 14:28:15 CDTGO:0042493 response to drug (P)PMID:22363646IMP

Figure 4: Knockdown of SIRT1 allows the cells to become sensitive to the drug. Data shows that SIRT1 allows the cell to be drug resistant.

challenge
requireschangesHUMAN:SIRT12012-03-13 14:37:29 CDTGO:0043066 negative regulation of apoptotic process (P)PMID:22363646IEP

Figure 5A-C: overexpression of SIRT1 allows for the downregulation of apoptosis, allowing for ATF4-induced multi-drug resistance in gastric cancer.

challenge
acceptableRAT:FAS2012-04-10 12:38:33 CDTGO:0004312 fatty acid synthase activity (F)PMID:21389266IDA

Figure 1

challenge
acceptable:1
unacceptable:1
requires_changes:4
flagged:0

Annotations challenged by Dahle001

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesYuen0005,
Team Rico FTW
HUMAN:SRCGO:0004715 - non-membrane spanning protein tyrosine kinase activity (F)PMID:22303479IDA challengeC: 1
0 annotations fixed by Dahle001