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TAIR:PAS1
Contents |
| Species (Taxon ID) | Arabidopsis thaliana (thale cress) (taxon:3702) | |
| Gene Name(s) | PAS1 ( synonyms: AT3G54010, PAS1, DEI1, PASTICCINO 1, F5K20.310, AT3G54010.1, AT3G54010.2 ) | |
| Protein Name(s) | AT3G54010, PASTICCINO 1, | |
| External Links | ||
| TAIR | locus:2084435 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
Figure 3 shows the process by which the study confirmed the interactions of PAS1 and FAN, a transcription factor. 3(A) shows two Western blots. The left Western blot was done after the researchers combined FAN attached to amylose beads with a PAS1 fusion protein. PAS1 was found to bind the beads with FAN, and not the blank beads. The right Western shows a second, similar study. This time, FAN was used in a column that ran the entire protein extract of cells expressing a PAS1-GFP fusion protein. Again, PAS1-GFP was retained in the presence of FAN and was not retained by matrix alone. These in vitro studies show that PAS1 and FAN interact strongly, since the interaction was retained between two different experimental conditions. Further, the in vivo interaction of these proteins was tested, as seen in 3(F). The proteins consistently co-localized when expressed in leaves, and through the use of FRET (fluorescence resonance energy transfer), with PAS1-GFP and DsRED-FAN as the paired fluorescing fusion molecules. In mutants in which the C-terminal domain is eliminated, binding does not occur (Figure 3(B)). Fluorescence increased significantly (by up to 22%) in the wild type PAS1 condition when compared to the C-terminal deletion mutant, indicating that binding occurs in vivo and confirming the importance of the C-terminal region for PAS1 binding to FAN. |
complete | |||
| GO:0000413 |
protein peptidyl-prolyl isomerization |
TAIR:Communication:501741973 |
PANTHER:PTHR10516_AN0 |
P |
From TAIR |
|||
| GO:0003755 |
peptidyl-prolyl cis-trans isomerase activity |
TAIR:Communication:501714663 |
ISS: Inferred from Sequence or Structural Similarity |
INTERPRO:IPR001179 |
F |
From TAIR |
||
| GO:0003755 |
peptidyl-prolyl cis-trans isomerase activity |
TAIR:Communication:501741973 |
PANTHER:PTHR10516_AN0 |
F |
From TAIR |
|||
| GO:0005515 |
protein binding |
TAIR:Publication:501731612 |
IPI: Inferred from Physical Interaction |
F |
From TAIR |
|||
| GO:0005528 |
FK506 binding |
TAIR:Communication:501714663 |
ISS: Inferred from Sequence or Structural Similarity |
INTERPRO:IPR001179 |
F |
From TAIR |
||
| GO:0005528 |
FK506 binding |
TAIR:Communication:501741973 |
PANTHER:PTHR10516_AN0 |
F |
From TAIR |
|||
| GO:0005634 |
nucleus |
TAIR:Publication:2647 |
ISS: Inferred from Sequence or Structural Similarity |
C |
From TAIR |
|||
| GO:0005783 |
endoplasmic reticulum |
TAIR:Publication:501736196 |
IDA: Inferred from Direct Assay |
C |
From TAIR |
|||
| GO:0005829 |
cytosol |
TAIR:Publication:501741191 |
IDA: Inferred from Direct Assay |
C |
From TAIR |
|||
| GO:0005829 |
cytosol |
TAIR:Publication:501741191 |
IDA: Inferred from Direct Assay |
C |
From TAIR |
|||
| GO:0006457 |
protein folding |
TAIR:AnalysisReference:501748310 |
IEA: Inferred from Electronic Annotation |
INTERPRO:IPR001179 |
P |
From TAIR |
||
| GO:0006457 |
protein folding |
TAIR:AnalysisReference:501748310 |
IEA: Inferred from Electronic Annotation |
INTERPRO:IPR001179 |
P |
From TAIR |
||
| GO:0009735 |
response to cytokinin stimulus |
TAIR:Publication:2643 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0009790 |
embryo development |
TAIR:Publication:2643 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0009826 |
unidimensional cell growth |
TAIR:Publication:2643 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0009880 |
embryonic pattern specification |
TAIR:Publication:501736196 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0016020 |
membrane |
TAIR:Communication:501741973 |
PANTHER:PTHR10516_AN0 |
C |
From TAIR |
|||
| GO:0018208 |
peptidyl-proline modification |
TAIR:Communication:501741973 |
PANTHER:PTHR10516_AN0 |
P |
From TAIR |
|||
| GO:0030010 |
establishment of cell polarity |
TAIR:Publication:501736196 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0030154 |
cell differentiation |
TAIR:Publication:2643 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0042761 |
very long-chain fatty acid biosynthetic process |
TAIR:Publication:501736196 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0048364 |
root development |
TAIR:Publication:2643 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
|||
| GO:0048527 |
lateral root development |
TAIR:Publication:501736196 |
IMP: Inferred from Mutant Phenotype |
P |
From TAIR |
| ||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Smyczynski C et al. (2006) The C terminus of the immunophilin PASTICCINO1 is required for plant development and for interaction with a NAC-like transcription factor. J Biol Chem 281: 25475-84 PubMed GONUTS page
- ↑ Vittorioso P et al. (1998) Mutation in the Arabidopsis PASTICCINO1 gene, which encodes a new FK506-binding protein-like protein, has a dramatic effect on plant development. Mol Cell Biol 18: 3034-43 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 3.4 Roudier F et al. (2010) Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. Plant Cell 22: 364-75 PubMed GONUTS page
- ↑ 4.0 4.1 Ito J et al. (2011) Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism. J Proteome Res 10: 1571-82 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 5.3 5.4 Faure JD et al. (1998) The PASTICCINO genes of Arabidopsis thaliana are involved in the control of cell division and differentiation. Development 125: 909-18 PubMed GONUTS page
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