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PMID:9813183
From GONUTS
Chae, SC, Inazu, Y, Amagai, A and Maeda, Y (1998) Underexpression of a novel gene, dia2, impairs the transition of Dictyostelium cells from growth to differentiation.Biochem. Biophys. Res. Commun. 252:278-83
| Abstract | In Dictyostelium discoideum Ax-2 cells, a specific point (PS-point) in the cell cycle from which they initiate differentiation in response to starvation has been specified. Using synchronized Ax-2 cells and the differential display method, a novel gene (differentiation-associated gene 2; dia2) was isolated as one of the genes expressed specifically during the shift of Ax-2 cells from growth to differentiation. The dia2 gene codes a lysine- and leucine-rich protein with a predicted molecular mass of 16.9 kDa. Northern blot analysis has shown that the dia2 mRNA, of 0.7 kb, accumulates in differentiating cells starved just before the PS-point, while there is no detectable expression in vegetatively growing cells. Antisense-mediated gene inactivation of dia2 greatly inhibited the progress of differentiation, presumably through the reduced expression of cAMP receptor 1 (car1). Thus, the DIA2 expression was suggested to have an essential role in the initiation of differentiation, closely relating to the cAMP signaling system. |
| Links | PubMed Online version:10.1006/bbrc.1998.9461 |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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References
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