PMID:9139699

Citation	Nibbs, RJ, Wylie, SM, Pragnell, IB and Graham, GJ (1997) Cloning and characterization of a novel murine beta chemokine receptor, D6. Comparison to three other related macrophage inflammatory protein-1alpha receptors, CCR-1, CCR-3, and CCR-5. J. Biol. Chem. 272:12495-504
Abstract	The beta-chemokine macrophage inflammatory protein-1alpha (MIP-1alpha) is chemotactic for many hemopoietic cell types and can inhibit hemopoietic stem cell (HSC) proliferation, effects mediated through G-protein coupled heptahelical receptors. We have isolated cDNAs for seven chemokine receptors, CCR-1 to -5, MIP-1alphaRL1, and a novel cDNA, D6. Chinese hamster ovary cells expressing CCR-1, -3, -5, and D6 bound 125I-murine MIP-1alpha: the order of affinity was D6 > CCR-5 > CCR-1 > CCR-3. Each bound a distinct subset of other beta-chemokines: the order of competition for 125I-murine MIP-1alpha on D6 was murine MIP-1alpha > human and murine MIP-1beta > human RANTES approximately JE > human MCP-3 > human MCP-1. Human MIP-1alpha and the alpha-chemokines did not compete. Like other chemokine receptors, D6 induced transient increases in [Ca2+] in HEK 293 cells upon ligand binding. D6 mRNA was abundant in lung and detectable in many other tissues. Bone marrow cell fractionation demonstrated T-cell and macrophage/monocyte expression of D6, and CCR-1, -3, and -5. Moreover, we could detect expression of CCR-3, CCR-5, and to a greater extent D6 in a cell population enriched for HSCs. Thus, we have characterized four murine beta chemokine receptors that are likely involved in mediating the pro-inflammatory functions of MIP-1alpha and other chemokines, and we present D6, CCR-3, and CCR-5 as candidate receptors in MIP-1alpha-induced HSC inhibition.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Base Sequence; Bone Marrow/chemistry; CHO Cells; Chemokine CCL3; Chemokine CCL4; Cloning, Molecular; Cricetinae; DNA, Complementary/chemistry; Hematopoietic Stem Cells/chemistry; Humans; Macrophage Inflammatory Proteins/chemistry; Macrophage Inflammatory Proteins/genetics; Mice; Molecular Sequence Data; Receptors, CCR10; Receptors, CCR3; Receptors, CCR5; Receptors, CCR8; Receptors, Chemokine; Receptors, Cytokine/chemistry; Receptors, Cytokine/genetics; Receptors, HIV/chemistry; Receptors, HIV/genetics; Sequence Alignment
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