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PMID:11557311
| Citation |
Behndig, A, Karlsson, K, Reaume, AG, Sentman, ML and Marklund, SL (2001) In vitro photochemical cataract in mice lacking copper-zinc superoxide dismutase. Free Radic. Biol. Med. 31:738-44 |
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| Abstract |
We here evaluate cataract formation in mice lacking the cytosolic copper-zinc superoxide dismutase (CuZn-SOD) in an in vitro model using irradiation with visible light and riboflavin as a photosensitizing agent. Isolated, cultured lenses from wild-type and CuZn-SOD-null mice were irradiated for 1.5 h by a daylight fluorescent light after preincubation with 10 microM riboflavin for 24 h. Cataract formation was evaluated daily with digital image analysis and ocular staging, and after 5 d 86Rb uptake and water contents of the lenses were determined. Basal superoxide concentrations in freshly isolated lenses from wild-type and CuZn-SOD-null mice were determined with lucigenin-derived chemiluminescense, and enzymatic activities of all three SOD isoenzymes in the murine lens were determined with a direct spectrophotometric method. The cytosolic CuZn-SOD accounts for 90% of the total SOD activity of the murine lens. CuZn-SOD-null lenses showed a doubled basal superoxide concentration, and were more prone to develop photochemical cataract in the present model with more opacification, more hydration, and less 86Rb uptake than lenses from wild-type mice. We conclude that CuZn-SOD is an important superoxide scavenger in the lens, and that it may have a protective role against cataract formation. |
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| Keywords |
Acridines; Animals; Body Water; Cataract/enzymology; Cataract/etiology; Cataract/pathology; Lens, Crystalline/chemistry; Lens, Crystalline/metabolism; Lens, Crystalline/pathology; Light; Luminescent Measurements; Mice; Mice, Knockout; Organ Culture Techniques; Photochemistry; Photosensitizing Agents/administration & dosage; Riboflavin/administration & dosage; Rubidium Radioisotopes/metabolism; Superoxide Dismutase/deficiency; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism; Superoxides/analysis |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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