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LACCA:PTLCB
Contents |
| Species (Taxon ID) | Lactobacillus casei. (1582) | |
| Gene Name(s) | lacE | |
| Protein Name(s) | PTS system lactose-specific EIICB component
EIICB-Lac EII-Lac Lactose permease IIC component PTS system lactose-specific EIIC component Lactose-specific phosphotransferase enzyme IIB component PTS system lactose-specific EIIB component | |
| External Links | ||
| UniProt | P24400 | |
| EMBL | M60851 | |
| PIR | B23697 | |
| ProteinModelPortal | P24400 | |
| GO | GO:0016021 GO:0005886 GO:0016301 GO:0008982 GO:0005529 GO:0005351 GO:0009401 | |
| InterPro | IPR014350 IPR003501 IPR013012 IPR003352 IPR004501 IPR004801 | |
| Pfam | PF02378 PF02302 | |
| TIGRFAMs | TIGR00394 TIGR00410 TIGR00853 | |
| PROSITE | PS51100 PS51105 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005351 |
sugar:hydrogen symporter activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005529 |
sugar binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0039 |
C |
Seeded From UniProt |
|||
| GO:0006810 |
transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0008643 |
carbohydrate transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016301 |
kinase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0016310 |
phosphorylation |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0016740 |
transferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0016310 |
phosphorylation |
IMP: Inferred from Mutant Phenotype |
P |
Fig. 5 Lanes 1-4 indicate phosphorylation activity. |
complete | |||
| GO:0046835 |
carbohydrate phosphorylation |
IMP: Inferred from Mutant Phenotype |
P |
As seen in figure 7, lanes 1 through 6, when each cysteine residue was replaced in turn by serine, lactose phosphorylation was found in mutants in which cysteine residues 31, 137, 152, 235, 316, or 349 had been replaced. As seen in figure 7, lane 7, no phosphorylation of the substrate occurred when Cys483 was replaced with a serine. As seen in figure 7, lanes 7 through 9, to confirm that this effect was not due to an undetected secondary mutation, a total of five independently obtained Cys483+Ser483 mutants, from two different experiments, were assayed. It was found in all cases that these mutants did not phosphorylate lactose. As seen in figure 7, lanes 10 through 11, when Cys483 was replaced with histidine, an amino acid found to be involved in all other phosphoryltransfer reactions of the PTS, or tyrosine, no lactose phosphorylation was observed. The loss of phosphorylating activity appeared to be specifically due to the exchange of Cys483 and not due to disruption of the tertiary structure of the protein. Cys483 is the active center phosphorylated amino acid residue in EIIlacL.casei. |
complete | |||
| GO:0005886 |
plasma membrane |
PMID:PMC2753894 |
IMP: Inferred from Mutant Phenotype |
C |
Figure 5: Presence of intact peptidoglycan |
complete | ||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Alpert CA & Chassy BM (1990) Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific enzyme II of the phosphotransferase system of Lactobacillus casei. Evidence that a cysteine residue is essential for sugar phosphorylation. J Biol Chem 265: 22561-8 PubMed GONUTS page
