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LACCA:PTLCB

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Species (Taxon ID) Lactobacillus casei. (1582)
Gene Name(s) lacE
Protein Name(s) PTS system lactose-specific EIICB component

EIICB-Lac EII-Lac Lactose permease IIC component PTS system lactose-specific EIIC component Lactose-specific phosphotransferase enzyme IIB component PTS system lactose-specific EIIB component

External Links
UniProt P24400
EMBL M60851
PIR B23697
ProteinModelPortal P24400
GO GO:0016021
GO:0005886
GO:0016301
GO:0022869
GO:0046835
GO:0009401
GO:0016310
InterPro IPR003501
IPR013012
IPR003352
IPR004501
IPR004801
Pfam PF02378
PF02302
SUPFAM SSF52794
TIGRFAMs TIGR00394
TIGR00410
TIGR00853
PROSITE PS51100
PS51105

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0016310

phosphorylation

PMID:2125053[1]

ECO:0000315

P

Fig. 5 Lanes 1-4 indicate phosphorylation activity.

complete
CACAO 3659

GO:0046835

carbohydrate phosphorylation

PMID:2125053[1]

ECO:0000315

P

As seen in figure 7, lanes 1 through 6, when each cysteine residue was replaced in turn by serine, lactose phosphorylation was found in mutants in which cysteine residues 31, 137, 152, 235, 316, or 349 had been replaced. As seen in figure 7, lane 7, no phosphorylation of the substrate occurred when Cys483 was replaced with a serine. As seen in figure 7, lanes 7 through 9, to confirm that this effect was not due to an undetected secondary mutation, a total of five independently obtained Cys483+Ser483 mutants, from two different experiments, were assayed. It was found in all cases that these mutants did not phosphorylate lactose. As seen in figure 7, lanes 10 through 11, when Cys483 was replaced with histidine, an amino acid found to be involved in all other phosphoryltransfer reactions of the PTS, or tyrosine, no lactose phosphorylation was observed. The loss of phosphorylating activity appeared to be specifically due to the exchange of Cys483 and not due to disruption of the tertiary structure of the protein. Cys483 is the active center phosphorylated amino acid residue in EIIlacL.casei.

complete
CACAO 4834

GO:0005886

plasma membrane

PMID:PMC2753894

ECO:0000315

C

Figure 5: Presence of intact peptidoglycan

complete

involved_in

GO:0046835

carbohydrate phosphorylation

PMID:2125053[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0016310

phosphorylation

PMID:2125053[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0015767

lactose transport

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0022869

P

Seeded From UniProt

complete

involved_in

GO:0034219

carbohydrate transmembrane transport

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0008982

P

Seeded From UniProt

complete

enables

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003352
InterPro:IPR003501
InterPro:IPR004501
InterPro:IPR004801
InterPro:IPR013012
InterPro:IPR036095

F

Seeded From UniProt

complete

involved_in

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003352
InterPro:IPR003501
InterPro:IPR004501
InterPro:IPR013012
InterPro:IPR036095

P

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003352

C

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004501
InterPro:IPR004801

C

Seeded From UniProt

complete

enables

GO:0022869

protein-N(PI)-phosphohistidine-lactose phosphotransferase system transporter activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004801

F

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

enables

GO:0016301

kinase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0418

F

Seeded From UniProt

complete

involved_in

GO:0016310

phosphorylation

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0418

P

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

involved_in

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0598

P

Seeded From UniProt

complete

involved_in

GO:0008643

carbohydrate transport

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0762

P

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1003
UniProtKB-SubCell:SL-0039

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 Alpert, CA & Chassy, BM (1990) Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific enzyme II of the phosphotransferase system of Lactobacillus casei. Evidence that a cysteine residue is essential for sugar phosphorylation. J. Biol. Chem. 265 22561-8 PubMed GONUTS page