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HUMAN:TX1B3
Contents |
| Species (Taxon ID) | Homo sapiens (Human). (taxon:9606) | |
| Gene Name(s) | TAX1BP3 ( synonyms: TIP1 ) | |
| Protein Name(s) |
| |
| External Links | ||
| UniProt Identifier | TX1B3_HUMAN | |
| UniProt Accessions | O14907, B2RD53, D3DTJ6, Q7LCQ4, | |
| EMBL | AF028823, AF168787, AF234997, AF277318, AK315408, CH471108, CH471108, BC023980, | |
| RefSeq | NP_001191627.1, NP_055419.1, | |
| PDB | 2KG2, 2L4S, 2L4T, 2VZ5, 3GJ9, | |
| IntAct | O14907, | |
| Ensembl | ENST00000225525, | |
| Pfam | PF00595, | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737 |
cytoplasm |
NAS: Non-traceable Author Statement |
C |
Source: UniProtKB |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
Source: UniProtKB-SubCell |
||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Source: UniProtKB |
||||
| GO:0030178 |
negative regulation of Wnt receptor signali... |
ISS: Inferred from Sequence or Structural Similarity |
P |
Source: UniProtKB |
||||
| GO:0007266 |
Rho protein signal transduction |
IDA: Inferred from Direct Assay |
P |
Source: UniProtKB |
||||
| GO:0016055 |
Wnt receptor signaling pathway |
IEA: Inferred from Electronic Annotation |
P |
Source: UniProtKB-KW |
||||
| GO:0030165 |
PDZ domain binding |
21139582 |
IPI: Inferred from Physical Interaction |
UniProtKB:O14907 UniProtKB:Q5VV41
|
F |
Figure 1 Interactions, regulation and stability of GEF16. (A) Yeast mating assay confirming that Tip-1 binds to short carboxyl terminal GEF16 fragments isolated from the primary Tip-1 yeast two-hybrid screen. (B) Glutathione transferase pull-down of Tip-1 with IVTT full-length wildtype and mutant GEF16 (wtGEF16/mutGEF16). The wtGEF16 contains the PDZ-binding domain ETDV, whereas mutGEF16 has the TDV residues deleted. Tip-1-GST fusion protein and GST control protein were bound to glutathione-sepharose beads. These were mixed with equal amounts of the IVTT wtGEF16 or mutGEF16 proteins, and then washed with binding buffer. Bound proteins were separated by SDS–PAGE and visualised by western immunoblotting with anti-GEF16. The GST-Tip-1 protein bound to the wtGEF16, but did not bind to the mutant form, whereas the GST control did not bind either product |
complete | |
| GO:0043088 |
regulation of Cdc42 GTPase activity |
IDA: Inferred from Direct Assay |
P |
Fluorescent kinetic analysis of Cdc42 activation by GEF16 in the presence of high-risk HPV16 E6 and Tip-1. Different combinations of 2 mM concentrations of the recombinant proteins GEF16, Tip-1 and HPV16 E6 were added after 4 min of initial readings being taken with Cdc42 and assay buffers alone. Each combination was carried out in triplicate. It can be seen that Cdc42 activation by GEF16 is enhanced in the presence of Tip-1 and E6. HPV16 E6, Tip-1, ARHGEF16 and Cdc42 activation. |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
|||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
|||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
|||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
|||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000047410 |
C |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0191 |
C |
||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000047410 |
C |
||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
||||
| GO:0005737 |
cytoplasm |
NAS: Non-traceable Author Statement |
C |
|||||
| GO:0007266 |
Rho protein signal transduction |
IDA: Inferred from Direct Assay |
P |
|||||
| GO:0008013 |
beta-catenin binding |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000047410 |
F |
||||
| GO:0008022 |
protein C-terminus binding |
IPI: Inferred from Physical Interaction |
F |
|||||
| GO:0008285 |
negative regulation of cell proliferation |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000047410 |
P |
||||
| GO:0016055 |
Wnt receptor signaling pathway |
IEA: Inferred from Electronic Annotation |
P |
|||||
| GO:0030178 |
negative regulation of Wnt receptor signaling pathway |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000047410 |
P |
||||
| GO:0030178 |
negative regulation of Wnt receptor signaling pathway |
ISS: Inferred from Sequence or Structural Similarity |
P |
|||||
| GO:0032864 |
activation of Cdc42 GTPase activity |
IDA: Inferred from Direct Assay |
P |
|||||
| GO:2000009 |
negative regulation of protein localization at cell surface |
IDA: Inferred from Direct Assay |
P |
| ||||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 Oliver AW et al. (2011) The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br J Cancer 104: 324-31 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Reynaud C et al. (2000) The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element. J Biol Chem 275: 33962-8 PubMed GONUTS page
- ↑ Olalla L et al. (2001) The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins. FEBS Lett 488: 116-22 PubMed GONUTS page
- ↑ 4.0 4.1 Alewine C et al. (2006) TIP-1 has PDZ scaffold antagonist activity. Mol Biol Cell 17: 4200-11 PubMed GONUTS page
