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HUMAN:MK67I
Contents |
| Species (Taxon ID) | Homo sapiens (Human). ([1]) | |
| Gene Name(s) | MKI67IP (synonyms: NIFK, NOPP34) | |
| Protein Name(s) | MKI67 FHA domain-interacting nucleolar phosphoprotein
Nucleolar phosphoprotein Nopp34 Nucleolar protein interacting with the FHA domain of pKI-67 hNIFK | |
| External Links | ||
| EMBL | AB044971 AK291903 AC018737 CH471103 BC012457 BC022990 BC024238 | |
| IPI | IPI00154590 | |
| RefSeq | NP_115766.3 | |
| UniGene | Hs.367842 | |
| PDB | 2AFF | |
| PDBsum | 2AFF | |
| ProteinModelPortal | Q9BYG3 | |
| SMR | Q9BYG3 | |
| DIP | DIP-28134N | |
| IntAct | Q9BYG3 | |
| MINT | MINT-1031770 | |
| STRING | Q9BYG3 | |
| PhosphoSite | Q9BYG3 | |
| SWISS-2DPAGE | Q9BYG3 | |
| PeptideAtlas | Q9BYG3 | |
| PRIDE | Q9BYG3 | |
| Ensembl | ENST00000285814 | |
| GeneID | 84365 | |
| KEGG | hsa:84365 | |
| CTD | 84365 | |
| GeneCards | GC02M114807 | |
| H-InvDB | HIX0002423 | |
| HGNC | HGNC:17838 | |
| HPA | CAB015382 | |
| MIM | 611970 | |
| neXtProt | NX_Q9BYG3 | |
| PharmGKB | PA38470 | |
| eggNOG | prNOG06977 | |
| GeneTree | ENSGT00390000011515 | |
| HOGENOM | HBG745405 | |
| HOVERGEN | HBG057796 | |
| InParanoid | Q9BYG3 | |
| OMA | PPEKVHK | |
| OrthoDB | EOG4MKNGX | |
| PhylomeDB | Q9BYG3 | |
| NextBio | 74141 | |
| ArrayExpress | Q9BYG3 | |
| Bgee | Q9BYG3 | |
| CleanEx | HS_MKI67IP | |
| Genevestigator | Q9BYG3 | |
| GermOnline | ENSG00000155438 | |
| GO | GO:0000794 GO:0005737 GO:0005730 GO:0005654 GO:0000166 GO:0005515 GO:0003723 GO:0006461 GO:0016072 GO:0009303 | |
| InterPro | IPR021043 IPR012677 IPR000504 | |
| Gene3D | G3DSA:3.30.70.330 | |
| Pfam | PF12196 PF00076 | |
| SMART | SM00360 | |
| PROSITE | PS50102 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0000794 |
condensed nuclear chromosome |
IDA: Inferred from Direct Assay |
|
C |
Seeded From UniProt |
|||
| GO:0003676 |
nucleic acid binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0003723 |
RNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0003723 |
RNA binding |
IDA: Inferred from Direct Assay |
|
F |
Seeded From UniProt |
|||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded From UniProt |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005654 |
nucleoplasm |
IDA: Inferred from Direct Assay |
|
C |
Seeded From UniProt |
|||
| GO:0005694 |
chromosome |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005694 |
chromosome |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0468 |
C |
Seeded From UniProt |
|||
| GO:0005730 |
nucleolus |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0188 |
C |
Seeded From UniProt |
|||
| GO:0005730 |
nucleolus |
IDA: Inferred from Direct Assay |
|
C |
Seeded From UniProt |
|||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
|
C |
Seeded From UniProt |
|||
| GO:0006461 |
protein complex assembly |
NAS: Non-traceable Author Statement |
|
P |
Seeded From UniProt |
|||
| GO:0009303 |
rRNA transcription |
NAS: Non-traceable Author Statement |
|
P |
Seeded From UniProt |
|||
| GO:0016072 |
rRNA metabolic process |
NAS: Non-traceable Author Statement |
|
P |
Seeded From UniProt |
|||
| GO:0071530 |
FHA domain-mediated complex assembly |
IDA: Inferred from Direct Assay |
P |
Figure 2. Experiment performed in which cultures were analyzed by immunoblotting antibody-specific for hNIFK. Proteins that interacted with Ki-FHA were pulled down with a GST-Ki-FHA fusion protein and subsequently analyzed with Western blot with the anti-NIFK antibodies. As shown in Fig. 2 A, the mitotic forms of hNIFK (bands I and II) were pulled down a sufficient amount to conclude that hNIFK interacts efficiently with Ki-HFA binding domain. |
complete | |||
| GO:0016310 |
phosphorylation |
IMP: Inferred from Mutant Phenotype |
P |
Figure 5. Immunoblotting experiment conducted to see whether hNIFK binding to pKi-67 is dependent on mitotic phosphorylation of hNIFK. Interacting proteins had bars that were pulled down n immunoblot. NIFK was pulled down efficiently (Fig. 5A) T234A and T238A mutants of hNIFK were pulled down less efficiently - proving mitotic phosphorylation is a necessary component of binding between hNIFK and pi-67. |
complete | |||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Takagi M et al. (2001) A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis. J Biol Chem 276: 25386-91 PubMed GONUTS page
cfn |
n cont.
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p cont.r |
