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HUMAN:HSH2D
Contents |
| Species (Taxon ID) | Homo sapiens (Human). (taxon:9606) | |
| Gene Name(s) | HSH2D ( synonyms: ALX ) | |
| Protein Name(s) |
| |
| External Links | ||
| UniProt Identifier | HSH2D_HUMAN | |
| UniProt Accessions | Q96JZ2, B5ME72, Q6ZNG7, | |
| EMBL | AY319652, AK027792, AK131222, AC008894, AC020911, CH471106, BC025237, | |
| PIR | JC7781, | |
| RefSeq | NP_116244.1, | |
| PDB | 2CS0, | |
| Ensembl | ENST00000253680, | |
| Pfam | PF00017, | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0042110 |
T cell activation |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000071970 |
P |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0191 |
C |
||||
| GO:0005070 |
SH3/SH2 adaptor activity |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000071970 |
F |
||||
| GO:0006916 |
anti-apoptosis |
IDA: Inferred from Direct Assay |
P |
FIG. 1. HSH2 blocks BCR-induced apoptosis in WEHI-231 cells. A, WEHI-231 cells were transduced with retrovirus encoding the empty p-MSCV-puro vector (empty vector) or with virus containing either HSH2:p-MSCV-puro or Bcl-xL:p-MSCV-puro, after which the cells were cultured in the presence of puromycin to select for homogeneous expression of HSH2 or Bcl-xL. Empty vector control (open bar), HSH2- (gray bar), and Bcl-xL- (black bar) expressing WEHI-231 cells (1 � 105 cells/ml) were stimulated with polyclonal anti-IgM F(ab�)2 Ab for 24–48 h, after which cells were harvested and stained with PI to measure DNA content. Cells were analyzed using flow cytometry to quantitate the percentage of the total population that contained sub-G0/G1 levels of DNA at 24 and 48 h. B, quantitation of the percentage of viable cells (ANV�,PI�) at 0, 24, or 48 h after stimulation of empty vector control (●), HSH2 (f), or Bcl-xL (OE) WEHI-231 cells with anti-IgM F(ab�)2 Ab. C, quantitation of the percentage of control (open bar), HSH2- (gray bar), and Bcl-xL- (black bar) expressing WEHI-231 cells that are ANV�,PIlo: characteristic of early apoptotic cells. |
complete | |||
| GO:0005070 |
SH3/SH2 adaptor activity |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000071970 |
F |
||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0191 |
C |
||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
|||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
||||
| GO:0042110 |
T cell activation |
IEA: Inferred from Electronic Annotation |
Ensembl:ENSMUSP00000071970 |
P |
| |||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Herrin BR et al. (2005) The adaptor protein HSH2 attenuates apoptosis in response to ligation of the B cell antigen receptor complex on the B lymphoma cell line, WEHI-231. J Biol Chem 280: 3507-15 PubMed GONUTS page
