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HUMAN:GRP1
Contents |
| Species (Taxon ID) | Homo sapiens (Human). ([1]) | |
| Gene Name(s) | RASGRP1 (synonyms: RASGRP) | |
| Protein Name(s) | RAS guanyl-releasing protein 1
Calcium and DAG-regulated guanine nucleotide exchange factor II CalDAG-GEFII Ras guanyl-releasing protein | |
| External Links | ||
| EMBL | AF081195 AF081197 AF106071 AY634315 AY858556 AY954625 AY966005 BC067298 BC109296 BC109297 AB208848 | |
| IPI | IPI00024733 IPI00885089 IPI00885115 IPI00885132 IPI00885149 | |
| RefSeq | NP_001122074.1 NP_005730.2 | |
| UniGene | Hs.591127 | |
| HSSP | P28867 | |
| ProteinModelPortal | O95267 | |
| SMR | O95267 | |
| IntAct | O95267 | |
| STRING | O95267 | |
| PhosphoSite | O95267 | |
| PRIDE | O95267 | |
| Ensembl | ENST00000310803 | |
| GeneID | 10125 | |
| KEGG | hsa:10125 | |
| UCSC | uc001zkd.2 uc001zke.2 uc010bbf.1 uc010bbg.1 | |
| CTD | 10125 | |
| GeneCards | GC15M015624 | |
| H-InvDB | HIX0038076 | |
| HGNC | HGNC:9878 | |
| MIM | 152700 603962 | |
| neXtProt | NX_O95267 | |
| eggNOG | prNOG14400 | |
| GeneTree | ENSGT00510000046358 | |
| HOVERGEN | HBG007513 | |
| InParanoid | O95267 | |
| OMA | EESKDRT | |
| OrthoDB | EOG49ZXNS | |
| Pathway_Interaction_DB | tcrpathway cd8tcrpathway | |
| Reactome | REACT_13552 REACT_14797 REACT_604 REACT_6900 | |
| NextBio | 38299 | |
| ArrayExpress | O95267 | |
| Bgee | O95267 | |
| Genevestigator | O95267 | |
| GO | GO:0005829 GO:0005789 GO:0000139 GO:0005624 GO:0005886 GO:0005509 GO:0005085 GO:0008289 GO:0005515 GO:0030154 GO:0030168 GO:0007265 GO:0051056 | |
| InterPro | IPR020454 IPR011992 IPR018247 IPR018249 IPR002219 IPR000651 IPR008937 IPR023578 IPR001895 | |
| Gene3D | G3DSA:1.10.238.10 G3DSA:1.10.840.10 | |
| PANTHER | PTHR23113 | |
| Pfam | PF00130 PF00617 PF00618 | |
| PRINTS | PR00008 | |
| SMART | SM00109 SM00147 SM00229 | |
| SUPFAM | SSF48366 | |
| PROSITE | PS00018 PS50222 PS00720 PS50009 PS50212 PS00479 PS50081 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000139 |
Golgi membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0134 |
C |
Seeded From UniProt |
|||
| GO:0005085 |
guanyl-nucleotide exchange factor activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005085 |
guanyl-nucleotide exchange factor activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005085 |
guanyl-nucleotide exchange factor activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005085 |
guanyl-nucleotide exchange factor activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005085 |
guanyl-nucleotide exchange factor activity |
TAS: Traceable Author Statement |
|
F |
Seeded From UniProt |
|||
| GO:0005509 |
calcium ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005509 |
calcium ion binding |
TAS: Traceable Author Statement |
|
F |
Seeded From UniProt |
|||
| GO:0005622 |
intracellular |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005622 |
intracellular |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005622 |
intracellular |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005624 |
membrane fraction |
TAS: Traceable Author Statement |
|
C |
Seeded From UniProt |
|||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005783 |
endoplasmic reticulum |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005789 |
endoplasmic reticulum membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0097 |
C |
Seeded From UniProt |
|||
| GO:0005794 |
Golgi apparatus |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005829 |
cytosol |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0091 |
C |
Seeded From UniProt |
|||
| GO:0005829 |
cytosol |
Reactome:REACT_15507 |
TAS: Traceable Author Statement |
|
C |
Seeded From UniProt |
||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0039 |
C |
Seeded From UniProt |
|||
| GO:0007165 |
signal transduction |
TAS: Traceable Author Statement |
|
P |
Seeded From UniProt |
|||
| GO:0007264 |
small GTPase mediated signal transduction |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0007264 |
small GTPase mediated signal transduction |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0007265 |
Ras protein signal transduction |
TAS: Traceable Author Statement |
|
P |
Seeded From UniProt |
|||
| GO:0007596 |
blood coagulation |
Reactome:REACT_604 |
TAS: Traceable Author Statement |
|
P |
Seeded From UniProt |
||
| GO:0008289 |
lipid binding |
TAS: Traceable Author Statement |
|
F |
Seeded From UniProt |
|||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0030154 |
cell differentiation |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0030168 |
platelet activation |
Reactome:REACT_798 |
TAS: Traceable Author Statement |
|
P |
Seeded From UniProt |
||
| GO:0035556 |
intracellular signal transduction |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0043406 |
positive regulation of MAP kinase activity |
IMP: Inferred from Mutant Phenotype |
P |
Fig 4B. Erk1/Erk2 phosphorylation (via MAPK) was delayed and reduced in RasGRP1- mutants. |
complete | |||
| GO:0051056 |
regulation of small GTPase mediated signal transduction |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0010628 |
positive regulation of gene expression |
IMP: Inferred from Mutant Phenotype |
P |
Figure 3 |
complete | |||
| GO:0005794 |
Golgi apparatus |
IDA: Inferred from Direct Assay |
C |
Under the condition of serum starvation, RasGRP1 was predominantly diffusely distributed…with a small portion localized to the Golgi (Figure 5C). when PAQR10 or PAQR11 was coexpressed, the localization of RasGRP1 in the Golgi compartment was profoundly increased under unstimulated condition (Figure 5C). |
complete | |||
| GO:0001934 |
positive regulation of protein phosphorylation |
IDA: Inferred from Direct Assay |
P |
We found that the PAQR10/ PAQR11-induced ERK phosphorylation was inhibited by RasGRP1-specific siRNAs (Figure 5D). In Hela cells, RasGRP1-specific siRNA could also abrogate PAQR10/ PAQR11-mediated accumulation of GFP-RBD in the Golgi apparatus (Supplementary information, Figure S12) |
complete | |||
| GO:0032856 |
activation of Ras GTPase activity |
IMP: Inferred from Mutant Phenotype |
P |
Fig 3e; overexpression of RasGRP1 was induced to determine the protein's normal function |
complete | |||
| GO:0032856 |
activation of Ras GTPase activity |
IDA: Inferred from Direct Assay |
P |
Fig 4; overexpression of RasGRP1 -> hypersensitive Jurkat cells. However, we observed that RasGRP can activate Ras in vivo even in the presence of EGTA plus BAPTA/AM, compounds that sequester extracellular and intracellular calcium, respectively (data not shown). |
complete | |||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Kawasaki H et al. (1998) A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia. Proc Natl Acad Sci U S A 95: 13278-83 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 Ebinu JO et al. (1998) RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. Science 280: 1082-6 PubMed GONUTS page
- ↑ Roose JP et al. (2005) A diacylglycerol-protein kinase C-RasGRP1 pathway directs Ras activation upon antigen receptor stimulation of T cells. Mol Cell Biol 25: 4426-41 PubMed GONUTS page
- ↑ Markegard E et al. (2011) Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRα gene expression. PLoS One 6: e25540 PubMed GONUTS page
- ↑ 5.0 5.1 Jin T et al. (2012) PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus. Cell Res 22: 661-76 PubMed GONUTS page
- ↑ Bivona TG et al. (2003) Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1. Nature 424: 694-8 PubMed GONUTS page
- ↑ Ebinu JO et al. (2000) RasGRP links T-cell receptor signaling to Ras. Blood 95: 3199-203 PubMed GONUTS page
