HUMAN:ARHGG

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Species (Taxon ID)	Homo sapiens (Human). (taxon:9606)
Gene Name(s)	ARHGEF16 ( synonyms: EPHEXIN4, NBR )
Protein Name(s)	Rho guanine nucleotide exchange factor 16 Ephexin-4
External Links
UniProt Identifier	ARHGG_HUMAN
UniProt Accessions	Q5VV41, Q86TF0, Q99434,
EMBL	D89016, BT007270, AL512413, AL512413, BC002681, BC051838,
RefSeq	NP_055263.2,
PDB	1X6B,
IntAct	Q5VV41,
Ensembl	ENST00000378378,
Pfam	PF00169, PF00621, PF00018,

Annotations

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005829	cytosol		EXP: Inferred from Experiment		C	Source: Reactome
	GO:0005089	Rho guanyl-nucleotide exchange factor activity		IEA: Inferred from Electronic Annotation		F	Source: InterPro
	GO:0006915	apoptosis		EXP: Inferred from Experiment		P	Source: Reactome
	GO:0008624	induction of apoptosis by extracellular signals		EXP: Inferred from Experiment		P	Source: Reactome
	GO:0035023	regulation of Rho protein signal transduction		IEA: Inferred from Electronic Annotation		P	Source: InterPro
	GO:0017147	Wnt-protein binding	PMID:21139582^[1]	IPI: Inferred from Physical Interaction	UniProtKB:O14907 UniProtKB:Q5VV41	F	The introduction of this paper states that Tip-I has been shown to be a protein involved in the wnt(Reynaud et al,2000; Kanamori et al,2003) Experimental evidence in this paper show the interaction od TipI and GEF 16. Figure 1 Interactions, regulation and stability of GEF16. (A) Yeast mating assay confirming that Tip-1 binds to short carboxyl terminal GEF16 fragments isolated from the primary Tip-1 yeast two-hybrid screen. (B) Glutathione transferase pull-down of Tip-1 with IVTT full-length wildtype and mutant GEF16 (wtGEF16/mutGEF16). The wtGEF16 contains the PDZ-binding domain ETDV, whereas mutGEF16 has the TDV residues deleted. Tip-1-GST fusion protein and GST control protein were bound to glutathione-sepharose beads. These were mixed with equal amounts of the IVTT wtGEF16 or mutGEF16 proteins, and then washed with binding buffer. Bound proteins were separated by SDS–PAGE and visualised by western immunoblotting with anti-GEF16. The GST-Tip-1 protein bound to the wtGEF16, but did not bind to the mutant form, whereas the GST control did not bind either product.	complete
	GO:0043088		PMID:21139582^[1]	IDA: Inferred from Direct Assay			Fluorescent kinetic analysis of Cdc42 activation by GEF16 in the presence of high-risk HPV16 E6 and Tip-1. Different combinations of 2 mM concentrations of the recombinant proteins GEF16, Tip-1 and HPV16 E6 were added after 4 min of initial readings being taken with Cdc42 and assay buffers alone. Each combination was carried out in triplicate. It can be seen that Cdc42 activation by GEF16 is enhanced in the presence of Tip-1 and E6. HPV16 E6, Tip-1, ARHGEF16 and Cdc42 activation.	complete
	GO:0005085	guanyl-nucleotide exchange factor activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0344	F
	GO:0005089	Rho guanyl-nucleotide exchange factor activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000219	F
	GO:0005089	Rho guanyl-nucleotide exchange factor activity	PMID:20679435^[2]	IDA: Inferred from Direct Assay		F
	GO:0005515	protein binding	PMID:20679435^[2]	IPI: Inferred from Physical Interaction	UniProtKB:Q96JJ3	F
	GO:0005622	intracellular	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000219	C
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C
	GO:0005829	cytosol	Reactome:REACT_10098	TAS: Traceable Author Statement		C
	GO:0005829	cytosol	Reactome:REACT_13694	TAS: Traceable Author Statement		C
	GO:0005829	cytosol	Reactome:REACT_19216	TAS: Traceable Author Statement		C
	GO:0006915	apoptosis	Reactome:REACT_13638	TAS: Traceable Author Statement		P
	GO:0007264	small GTPase mediated signal transduction	Reactome:REACT_11044	TAS: Traceable Author Statement		P
	GO:0008624	induction of apoptosis by extracellular signals	Reactome:REACT_13720	TAS: Traceable Author Statement		P
	GO:0017048	Rho GTPase binding	PMID:20679435^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P63000	F
	GO:0017048	Rho GTPase binding	PMID:20679435^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P84095	F
	GO:0030165	PDZ domain binding	PMID:21139582^[1]	IPI: Inferred from Physical Interaction	UniProtKB:O14907	F
	GO:0030971	receptor tyrosine kinase binding	PMID:20679435^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P29317	F
	GO:0032863	activation of Rac GTPase activity	PMID:20679435^[2]	IMP: Inferred from Mutant Phenotype		P
	GO:0032864	activation of Cdc42 GTPase activity	PMID:21139582^[1]	IDA: Inferred from Direct Assay		P
	GO:0035023	regulation of Rho protein signal transduction	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000219	P
	GO:0048011	nerve growth factor receptor signaling pathway	Reactome:REACT_11061	TAS: Traceable Author Statement		P
	GO:0048011	nerve growth factor receptor signaling pathway	Reactome:REACT_13776	TAS: Traceable Author Statement		P
	GO:0051056	regulation of small GTPase mediated signal transduction	Reactome:REACT_11051	TAS: Traceable Author Statement		P
	GO:0060326	cell chemotaxis	PMID:20679435^[2]	IMP: Inferred from Mutant Phenotype		P
	GO:0090004	positive regulation of establishment of protein localization in plasma membrane	PMID:20679435^[2]	IMP: Inferred from Mutant Phenotype		P
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Oliver AW et al. (2011) The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br J Cancer 104: 324-31 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Hiramoto-Yamaki N et al. (2010) Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism. J Cell Biol 190: 461-77 PubMed GONUTS page

[PMID:21139582-0] 1.0 ^1.1 ^1.2 ^1.3 Oliver AW et al. (2011) The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br J Cancer 104: 324-31 PubMed GONUTS page

[PMID:20679435-1] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Hiramoto-Yamaki N et al. (2010) Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism. J Cell Biol 190: 461-77 PubMed GONUTS page

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HUMAN:ARHGG

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