ECOLI:DNAK

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Species (Taxon ID)	Escherichia coli (strain K12). (taxon:83333)
Gene Name(s)	dnaK ( synonyms: groP, grpF, seg )
Protein Name(s)	Chaperone protein DnaK HSP70 Heat shock 70 kDa protein Heat shock protein 70
External Links
UniProt Identifier	DNAK_ECOLI
UniProt Accessions	P0A6Y8, P04475,
EMBL	K01298, U00096, AP009048, D10765, M12565,
PIR	A03311,
RefSeq	AP_000678.1, NP_414555.1,
PDB	1BPR, 1DG4, 1DKG, 1DKX, 1DKY, 1DKZ, 1Q5L, 2BPR, 2KHO, 3DPO, 3DPP, 3DPQ, 3QNJ,
IntAct	P0A6Y8,
Pfam	PF00012,

Annotations

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P28304	F
	GO:0005886	plasma membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P68919	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0AG80	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:Q46906	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P00811	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P12282	F
	GO:0005886	plasma membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0037	C
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P00634	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0C0V0	F
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C
	GO:0006457	protein folding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	P
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P45578	F
	GO:0016020	membrane	PMID:16858726^[3]	IDA: Inferred from Direct Assay		C
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P62623	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P69924	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0ADX9	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0A6Z3	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P23721	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P69822	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:Q46796	F
	GO:0005515	protein binding	PMID:16139413^[4]	IPI: Inferred from Physical Interaction	UniProtKB:P21513	F
	GO:0009408	response to heat	PMID:8349564^[5]	IEP: Inferred from Expression Pattern		P
	GO:0016020	membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C
	GO:0051082	unfolded protein binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P46853	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AAI5	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P36677	F
	GO:0005515	protein binding	PMID:10521435^[6]	IPI: Inferred from Physical Interaction	UniProtKB:P08622	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0A853	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P76002	F
	GO:0005524	ATP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001023	F
	GO:0005515	protein binding	PMID:10521435^[6]	IPI: Inferred from Physical Interaction	UniProtKB:P08622	F
	GO:0016020	membrane	PMID:16858726^[3]	IDA: Inferred from Direct Assay		C
	GO:0006950	response to stress	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C
	GO:0005524	ATP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0ACX3	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P10346	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P16700	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P30011	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P37024	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P28635	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9Q9	F
	GO:0005886	plasma membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC81	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P37342	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC38	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P17117	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P37685	F
	GO:0005737	cytoplasm	PMID:16858726^[3]	IDA: Inferred from Direct Assay		C
	GO:0006260	DNA replication	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AB71	F
	GO:0008270	zinc ion binding	PMID:11985624^[7]	IDA: Inferred from Direct Assay		F
	GO:0005524	ATP binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F
	GO:0005515	protein binding	PMID:10521435^[6]	IPI: Inferred from Physical Interaction	UniProtKB:P08622	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0A853	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AAI5	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AB71	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC38	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC81	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0ACX3	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P0ADX9	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P12282	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P23721	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P28304	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P37342	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P45578	F
	GO:0005515	protein binding	PMID:15690043^[1]	IPI: Inferred from Physical Interaction	UniProtKB:P69924	F
	GO:0005515	protein binding	PMID:16139413^[4]	IPI: Inferred from Physical Interaction	UniProtKB:P21513	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P00634	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P00811	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0A6Z3	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9Q9	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0AG80	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P0C0V0	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P10346	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P16700	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P17117	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P28635	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P30011	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P36677	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P37024	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P37685	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P46853	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P62623	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P68919	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P69822	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:P76002	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:Q46796	F
	GO:0005515	protein binding	PMID:16606699^[2]	IPI: Inferred from Physical Interaction	UniProtKB:Q46906	F
	GO:0005524	ATP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001023	F
	GO:0005524	ATP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	F
	GO:0005524	ATP binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C
	GO:0005737	cytoplasm	PMID:16858726^[3]	IDA: Inferred from Direct Assay		C
	GO:0005886	plasma membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C
	GO:0005886	plasma membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C
	GO:0005886	plasma membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0037	C
	GO:0006260	DNA replication	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P
	GO:0006457	protein folding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	P
	GO:0006950	response to stress	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P
	GO:0016020	membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C
	GO:0016020	membrane	PMID:16858726^[3]	IDA: Inferred from Direct Assay		C
	GO:0051082	unfolded protein binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012725	F
	GO:0005524	ATP binding	PMID:10521435^[6]	IPI: Inferred from Physical Interaction		F	DnaK and DnaJ bind under certain circumstances- the abstract mentions that they have been shown to be co-chaperones. Figure 1, graph 1 demonstrates binding assays using immobilized DnaK on a sensor chip. The binding of DnaK to DnaJ occurs only in the presence of ATP, and not any other form of nucleotide. While this is not a direct analysis of the ATP-binding domain, from the graph it is possible to infer that DnaK requires the presence of ATP to bind DnaJ, and that the ATP is binding to DnaK (since it was only present in solution during the process of immobilizing DnaK, not when the DnaJ was added).	Missing: with/from
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed GONUTS page
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 ^2.21 ^2.22 ^2.23 ^2.24 ^2.25 ^2.26 ^2.27 ^2.28 ^2.29 ^2.30 ^2.31 ^2.32 ^2.33 ^2.34 ^2.35 ^2.36 ^2.37 ^2.38 ^2.39 ^2.40 ^2.41 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed GONUTS page
↑ ^4.0 ^4.1 Regonesi ME et al. (2006) Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie 88: 151-61 PubMed GONUTS page
↑ Chuang SE & Blattner FR (1993) Characterization of twenty-six new heat shock genes of Escherichia coli. J Bacteriol 175: 5242-52 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 ^6.3 Suh WC et al. (1999) Structural features required for the interaction of the Hsp70 molecular chaperone DnaK with its cochaperone DnaJ. J Biol Chem 274: 30534-9 PubMed GONUTS page
↑ Katayama A et al. (2002) Systematic search for zinc-binding proteins in Escherichia coli. Eur J Biochem 269: 2403-13 PubMed GONUTS page

[PMID:15690043-0] 1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed GONUTS page

[PMID:16606699-1] 2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 ^2.21 ^2.22 ^2.23 ^2.24 ^2.25 ^2.26 ^2.27 ^2.28 ^2.29 ^2.30 ^2.31 ^2.32 ^2.33 ^2.34 ^2.35 ^2.36 ^2.37 ^2.38 ^2.39 ^2.40 ^2.41 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed GONUTS page

[PMID:16858726-2] 3.0 ^3.1 ^3.2 ^3.3 ^3.4 Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed GONUTS page

[PMID:16139413-3] 4.0 ^4.1 Regonesi ME et al. (2006) Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie 88: 151-61 PubMed GONUTS page

[PMID:8349564-4] Chuang SE & Blattner FR (1993) Characterization of twenty-six new heat shock genes of Escherichia coli. J Bacteriol 175: 5242-52 PubMed GONUTS page

[PMID:10521435-5] 6.0 ^6.1 ^6.2 ^6.3 Suh WC et al. (1999) Structural features required for the interaction of the Hsp70 molecular chaperone DnaK with its cochaperone DnaJ. J Biol Chem 274: 30534-9 PubMed GONUTS page

[PMID:11985624-6] Katayama A et al. (2002) Systematic search for zinc-binding proteins in Escherichia coli. Eur J Biochem 269: 2403-13 PubMed GONUTS page

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ECOLI:DNAK

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