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Category:Team KEGGerators

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SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:LIPACavana55, Team KEGGerators2012-04-15 17:44:07 CDTGO:0016992 lipoate synthase activity (F)PMID:8444795ECO:0000314 direct assay evidence used in manual assertion

Figure 6 shows expression of lipA in a lipA+ strain compared to a mutant strain that is missing the lipA gene.

challenge
requireschangesECOLI:LPLACavana55, Team KEGGerators2012-04-15 17:04:44 CDTGO:0016979 lipoate-protein ligase activity (F)PMID:8206909ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows a defect in the accumulation of 36s-lipoyl proteins when lacking the lplA gene demonstrating lipoate-protein ligase activity.

challenge
acceptableSOLTU:G9JKC3Cavana55, Team KEGGerators2012-04-14 14:44:36 CDTGO:0035251 UDP-glucosyltransferase activity (F)PMID:9076990ECO:0000314 direct assay evidence used in manual assertion

Figure 8 shows an increase in solanidine UDP-glucosyltransferase activity in response to wounding of potato tuber tissue.

challenge
acceptableARATH:METK1Cavana55, Team KEGGerators2012-04-10 13:10:24 CDTGO:0004478 methionine adenosyltransferase activity (F)PMID:2535470ECO:0000314 direct assay evidence used in manual assertion

Table 1 shows the results of AdoMet Sythetase Assays where the stem extract contained 10 times more AdoMet Sythetase activity than in leaf extract. Along with figure 2(a) which showed increased expression of sam-1 gene in stem extracts compared to leaf extracts.

challenge
requireschangesECOLI:DMLACavana55, Team KEGGerators2012-04-10 12:11:15 CDTGO:0046553 D-malate dehydrogenase (decarboxylating) activity (F)PMID:17088549ECO:0000314 direct assay evidence used in manual assertion

Figure 2 (C) yeaU (dmlA) showed increase expression levels during growth on D-malate compared to growth on L-lactate providing evidence for its involvement in D-malate catabolism.

challenge
unacceptable9BURK:Q59I44Ropppat1, Team KEGGerators2012-04-10 11:58:52 CDTGO:0016491 oxidoreductase activity (F)PMID:15781461ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows the degradation of 2-Chloroacrylate with purified protein via mass spectrometric identification of products.

challenge
acceptableMALDO:CAS1Ropppat1, Team KEGGerators2012-04-10 11:33:35 CDTGO:0009611 response to wounding (P)PMID:17333023ECO:0000314 direct assay evidence used in manual assertion

Figure 5 (b) shows direct increase of mRNA transcript in response to mechanical wounding of the fruit with positive and negative controls.

challenge
requireschangesALCFA:AIOARopppat1, Team KEGGerators2012-04-10 11:08:20 CDTGO:0050611 arsenate reductase (azurin) activity (F)PMID:1331097ECO:0000314 direct assay evidence used in manual assertion

Figure 4 describes the steady state kinetics of the arsenate reductase activity in the presence of azurin.

challenge


MSU Internal Competition

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:PROACavana55, Team KEGGerators2012-03-29 15:53:19 CDTGO:0047443 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity (F)PMID:11826967ECO:0000314 direct assay evidence used in manual assertion

Figure 5 (B) shows a strain (containing the gene proA) grown in the presence of IPTG showing the same expression level as a purified strain of 4-hydroxy-4-methyl-2-oxoglutarate aldolase from P. ochraceae.

challenge

Pages in category "Team KEGGerators"

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