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Category:MichSt14A 48

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableYEAST:MUC1Santoro9, MichSt14A 482014-04-06 09:59:25 CDTGO:0030447 response to nitrate starvation (P)PMID:17921350ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C: Pictures of yeast plated. FLO11 gene controls hyphae (filaments) formation. WT grows hyphae in order to find more nutrients, while the knockout does not.

challenge
unacceptableYEAST:MUC1Santoro9, MichSt14A 482014-04-06 09:59:24 CDTGO:0043708 cell adhesion involved in biofilm formation (P)PMID:17921350ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1D: Adherence assay. Growing strain on plastic tests biofilm formation capabilities. There is growth seen on the WT plate but not with the knockout.

challenge
unacceptableYEAST:MUC1Santoro9, MichSt14A 482014-04-06 09:59:24 CDTGO:0016337 cell-cell adhesion (P)PMID:17921350ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 - Bead adhesion assay. Beads coated with the cells are placed on the plate to test cell-to-cell adhesion. In the WT, the growth is focused around the beads (black dots), while the growth in the knockout is not focused around the beads and is spread out around the plate.

challenge
unacceptableYEAST:MUC1Santoro9, MichSt14A 482014-04-06 09:59:24 CDTGO:0000128 flocculation (P)PMID:17921350ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A: Flocculation assay tests whether or not the cells aggregate together and drop to the bottom of the tube. The yeast does flocculate in the WT as the cells collect at the bottom. The knockout stays cloudy in the entire solution for the whole time.

challenge
unacceptableYEAST:MUC1Santoro9, MichSt14A 482014-04-06 09:59:23 CDTGO:0044409 entry into host (P)PMID:17921350ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B showed the sigma1278b strain plated onto agar before and after washing. The WT could grow into the agar while the flo11 knockout could not

challenge

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